F**********6
发帖数: 90 | 1 the major concern of transgenic RNAi (300-400 bp) in Drosophila is off-
target. In addition, Knock-down of some gene may kill the neuron. |
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a****d
发帖数: 1919 | 2 somethings you can take a look,
fruitless Splicing Specifies Male Courtship Behavior in Drosophila
Ebru Demir and Barry J. DicksonGo To Corresponding Author
The Sex-Determination Genes fruitless and doublesex Specify a Neural
Substrate Required for Courtship Song
Elizabeth J. Rideout1, Jean-Christophe Billeter1, 2 and Stephen F. Goodwin1,
Go To Corresponding Author |
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l***i
发帖数: 168 | 3 只知道
关于 zebrafish, 有 Zfin
关于 mouse， 有 MGI
另外，NCBI 和 Ensembl 是综合性的。
有关于其它常见实验动物模型的genome database吗，比如 drosophila , yeast？ 谢
谢。 |
|
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X***n
发帖数: 366 | 5 The two papers in the last issue of Science.
They seem just cataloging. Anything new? |
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M*****n
发帖数: 16729 | 6 since you are talking about fish, I'd like to throw in some 2 cents worth
opinions.
Indeed, no one can claim all the cells in the organism carry the exact same
DNA in their genome (of coure, here we are not focusing on somatic mutations which
are relatively rare). I just went to a talk today and learned that during
development of Drosophila, polyploid cells do exist and during the formation
of polypoid cells the genome undergoes "underreplication", which means not
the whole genome is replicated.... 阅读全帖 |
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M*****n
发帖数: 16729 | 7 NO,
those cells are in the hindgut of drosophila and they will divide. just learned from a talk I went.
replicated |
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M*****n
发帖数: 16729 | 8 this is better studied in Drosophila, but a fly is a fly.
don't know how much we can apply this to vertebrates. |
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M*****n
发帖数: 16729 | 9 sorry, I respect/envy Drosophila Genetics. |
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M*****n
发帖数: 16729 | 10 你这个见解应该去问那个fly lady,
问问他为啥当年不专心研究Drosophila, 反而要转行搞zebrafish genetics.
又快
一个
fis
/r
非常
optomot |
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s******r
发帖数: 2876 | 11 那他是为什么呢？
你这个见解应该去问那个fly lady,
问问他为啥当年不专心研究Drosophila, 反而要转行搞zebrafish genetics.
又快
一个
fis
/r
非常
optomot |
|
M*****n
发帖数: 16729 | 12 看neuron, drosophila worm都可以，不能算是fish 的advantage.
drug screening 比较靠谱，因为worm和fly的physiology与人比还是太远了，作为drug
screening俺觉得不是太合适，比鱼高等的model有，比如老鼠，但是large-scale drug screen的cost可能太大，所以现在fish 的最大的survival area就是chemical screening.
这个是本人的理解。所以fish 目前的方向就是disease modeling 和drug screening.
所谓发育生物学，那是上个世纪的事儿了，当然最近可是往regenerative/stem cell
biology 方向靠了，还是有点发展前途的。 |
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s*********y
发帖数: 1189 | 13 Cold Spring Harb Protoc. 2010 Apr;2010(4)
Isolation of Drosophila egg chambers for imaging.
Parton RM, Vallés AM, Dobbie IM, Davis I.
请发PDF 到 y******[email protected]
包子答谢。这里也先表示感谢！ |
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b****n
发帖数: 311 | 14 一大把的AP啊。列一下一作paper, 2,3作的paper就略掉了。Cold spring harbor
laboratory
再怎么说也不是三流烂校水平吧。大家乐一下吧，AP似乎没那么难，呵呵。
Mikala Egeblad:
http://www.cshl.edu/Faculty/egeblad-mikala.html
Egeblad, M., Ewald, A.J., Askautrud, H.A., Truitt, M.L., Welm, B.E.,
Bainbridge, E., Peeters, G., Krummel, M.F., and Werb, Z. 2008. Visualizing stromal
cell dynamics in different tumor microenvironments by spinning disk confocal
microscopy. Dis. Model. Mech. 1:155-16
Egeblad, M., Shen, H.-C. J., Behonick, D.J., Wilmes, L., Eichten, A.... 阅读全帖 |
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m****9
发帖数: 128 | 15 We submitted a paper to Cell and got response, the reviewer asked for in
vivo study data(in zebrafish,xenopus or drosophila)
We identified a tumor suppressor via activating TGF-b signaling, the
reviewer wanted to know how it affects tgf-b signaling in vivo.
Can anyone give some information about this or recommend some nice guys that
we can cooperate with? It is better in Boston area.
Thanks for help. |
|
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a****d
发帖数: 1919 | 17 You could work on both. |
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J********3
发帖数: 3151 | 18 epigenetics啊，现在是万能膏药，哪儿都能唬一下 |
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s*******r
发帖数: 562 | 19 同搭车求
Area: salivary gland physiology, channels/transporters, and Drosophila
Thank you! |
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e*******n
发帖数: 2178 | 20 我是做进化的，有个project想把另外一种drosophila的基因导入到melanogaster里面
，看看phenotype。现在知道另外一个物种这个基因应该是隐性的，所以需要把
melanogaster里面的内源性基因去掉。
查了一下好像2000年的一个science文章就建立了果蝇里面的knock out技术，但是使用
的人却寥寥无几。有人知道这个是什么原因吗？是这个技术本身不成熟，成功率低？
谢谢大家！:) |
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r**a
发帖数: 121 | 21 Diet restriction in Drosophila melanogaster. Design and analysis.
哪位好心人帮忙，发送到l***********[email protected]
感激不尽啊：） |
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s*********t
发帖数: 600 | 22 一个可能的诺贝尔化学奖
我们知道：诺贝尔化学奖委员会，不时地肯定化学和生物交叉的工作，比较常见的是生物化学和生物物理学的工作，有时也给分子生物学。
从2003到2009之间7年的诺贝尔化学奖，有5年给生物学研究：2003年钾通道的结构和水通道，2004年蛋白质降解，2006年基因转录的结构生物学研究，2008年绿色荧光蛋白，2009年合成蛋白质的核糖体结构。
结构生物学占了很大比重（2003、2006、2009）。
我们也知道：诺贝尔化学奖委员会经常犯错误，不该给的他们给了、该给的他们没给，两种错误都犯过。
2003年，不应该奖水通道的发现，因为并不足够突出：不是第一个通道（是第几十个通道）、也无特殊性。
2006年，化学家们只重视自己懂的，而忽略了同一科学领域中偏生物、但更重要的工作。基因转录领域，有两项工作的重要性毫无疑问高于解出转录因子的X线晶体结构：发现第一个转录因子（Mark Ptashne）、发现RNA多聚酶（Robert Roeder）。但诺贝尔化学奖委员会过分强调结构而忽略了转录领域中更重要的生物学工作。
基本可以放心：化学奖委员会一如既往地跨界出现错误，既不是第一次，... 阅读全帖 |
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s*********t
发帖数: 600 | 23 n 1990, Yi Rao published a paper on the cloning of Big Brain, a Drosophila
gene encoding a member of the
family which is later called aquaporins. So, Yi Rao is not unfamiliar with
this field.
Many channels are important and their deficits cause diseases.
The comparison of K channel by MacKinnon and water channel made by you is
off the point: the work on K
channel structure is important first because this was the first ion channel
whose structure has ever been
solved, not because of the clinical ... 阅读全帖 |
|
s*********t
发帖数: 600 | 24 一个可能的诺贝尔化学奖
我们知道：诺贝尔化学奖委员会，不时地肯定化学和生物交叉的工作，比较常见的是生物化学和生物物理学的工作，有时也给分子生物学。
从2003到2009之间7年的诺贝尔化学奖，有5年给生物学研究：2003年钾通道的结构和水通道，2004年蛋白质降解，2006年基因转录的结构生物学研究，2008年绿色荧光蛋白，2009年合成蛋白质的核糖体结构。
结构生物学占了很大比重（2003、2006、2009）。
我们也知道：诺贝尔化学奖委员会经常犯错误，不该给的他们给了、该给的他们没给，两种错误都犯过。
2003年，不应该奖水通道的发现，因为并不足够突出：不是第一个通道（是第几十个通道）、也无特殊性。
2006年，化学家们只重视自己懂的，而忽略了同一科学领域中偏生物、但更重要的工作。基因转录领域，有两项工作的重要性毫无疑问高于解出转录因子的X线晶体结构：发现第一个转录因子（Mark Ptashne）、发现RNA多聚酶（Robert Roeder）。但诺贝尔化学奖委员会过分强调结构而忽略了转录领域中更重要的生物学工作。
基本可以放心：化学奖委员会一如既往地跨界出现错误，既不是第一次，... 阅读全帖 |
|
s*********t
发帖数: 600 | 25 n 1990, Yi Rao published a paper on the cloning of Big Brain, a Drosophila
gene encoding a member of the
family which is later called aquaporins. So, Yi Rao is not unfamiliar with
this field.
Many channels are important and their deficits cause diseases.
The comparison of K channel by MacKinnon and water channel made by you is
off the point: the work on K
channel structure is important first because this was the first ion channel
whose structure has ever been
solved, not because of the clinical ... 阅读全帖 |
|
a*********f
发帖数: 101 | 26 Science. 2011 May 13;332(6031):845-8.
Experimental evidence supports a sex-specific selective sieve in
mitochondrial genome evolution.
Abstract
Mitochondria are maternally transmitted; hence, their genome can only make a
direct and adaptive response to selection through females, whereas males
represent an evolutionary dead end. In theory, this creates a sex-specific
selective sieve, enabling deleterious mutations to accumulate in
mitochondrial genomes if they exert male-specific effects. We test... 阅读全帖 |
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s******r
发帖数: 2876 | 27 这么说吧，如果有female只能生出female，那么male恐怕还是倾向于寻找正常的female
，不至于导致种族灭绝。
Science. 2011 May 13;332(6031):845-8.
Experimental evidence supports a sex-specific selective sieve in
mitochondrial genome evolution.
Abstract
Mitochondria are maternally transmitted; hence, their genome can only make a
direct and adaptive response to selection through females, whereas males
represent an evolutionary dead end. In theory, this creates a sex-specific
selective sieve, enabling deleterious mutations to accumulate in
mit... 阅读全帖 |
|
j********r
发帖数: 156 | 28 大侠来分析一下Pan的那篇g&d 和 Xu的NCB paper！俺愣是没看出名堂。
Pan的文章说，as its title says " Tsc2 is not a critical target of Akt during
normal Drosophila development"; xu的文章是 "Akt regulates growth by
directly
phosphorylating Tsc"
尽管都是用果蝇作模型，但focus的角度不一样 （the essential role of Akt during
developement vs the growth-stimulating function of Akt). Xu的文章也supported
by
many other papers. 而Akt底物众多，TSC2不能rescue 也不奇怪。
呵呵,结果被Duojia pan照脸打了一把. |
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y******8
发帖数: 1764 | 29 http://www.bio.pku.edu.cn/exchange/index.jsp
Chengqi Yi, Ph.D 主讲 Probing the Biology of Nucleic A
11:00 - 12:00, May. 19, 2011, Thu. 北大新生物楼517会议室
Suhua Feng, Ph.D 主讲 Studying DNA methylation in Eukaryotic Organisms
09:00 - 10:00, May. 19, 2011, Thu. 北大新生物楼517会议室
YuHang Chen, Ph.D 主讲 Exploring ion channels by crystallography and
electrophysiology
08:00 - 09:00, May. 19, 2011, Thu. 北大新生物楼517会议室
Xiang Yang, Ph.D 主讲 Novel Photoreception in Drosophila Larval Body Wall:
from Molecules to Behavior
10:0... 阅读全帖 |
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x*****7
发帖数: 58 | 30 求助 pAC5.1 insect expression vector for gene expression in drosophila.
I am in UF
My E-mail is l******[email protected]
If you have this vector, could you please share with me.
Thank you! |
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p*******7
发帖数: 134 | 31 is this cell paper?
1.Xia L, Jia S, Huang S, Wang H, Zhu Y, Mu Y, Kan L, Zheng W, Wu D, Li X,
Sun Q, Meng A, Chen D.* 2010,The Fused/Smurf complex controls the fate of
Drosophila germline stem cells by generating a gradient BMP response. Cell,
143(6):978-90. |
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A****A
发帖数: 16 | 32 目前的观点是，HR和NHEJ是在DNA双链断裂修复中互相竞争的pathways。如果能够有效
的抑制NHEJ,HR的频率有可能会有大幅度的提升。目前，一些labs主要通过knock out或
者knock down NHEJ中几个关键player， 比如，Ku70， Ku80 以及Ligase4，来进行验
证。比较convincing的data有Dana Carroll在果蝇的研究，以及Eric Hendrickson在
mammalian cell line中的研究。Paper 如下:
Proc Natl Acad Sci U S A. 2008 Jun 24;105(25):8703-8. Epub 2008 Jun 18.
Ku70, an essential gene, modulates the frequency of rAAV-mediated gene
targeting in human somatic cells.
Fattah FJ, Lichter NF, Fattah KR, Oh S, Hendrickson EA.
Genetics. 2009 Jul;1... 阅读全帖 |
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p*****m
发帖数: 7030 | 33 lol 江安世的文章我是看过的好不好。。。另外 饶毅什么地方说他觉得ROC是可以得了
？他明明说的是ROC不行 一定要China lol
A Map of Olfactory Representation in the Drosophila Mushroom Body
Hui-Hao Lin1, 2, Jason Sih-Yu Lai1, 2, An-Lun Chin1, 2, Yung-Chang Chen3, 4
and Ann-Shyn Chiang1, 2, 3, 5, 6, ,
1 Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013,
Taiwan, ROC
2 Department of Life Science, National Tsing Hua University, Hsinchu 30013,
Taiwan, ROC
3 Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwa
... 阅读全帖 |
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d*****r
发帖数: 2583 | 34 http://www.hhmi.org/news/popups/isrf_pop.html
2011 International Student Research Fellows
Institution Student Advisor Discipline
Boston University Wei Guo
China Barbara Shinn-Cunningham Systems and computational
neuroscience
Brandeis University Marcus Long
United Kingdom Lizbeth Hedstrom Protein degradation using chemical
biology
California Institute of Technology Andreas Hoenselaar
Germany Thanos Siapas Spatial mapping in the hippocampus using
virtual
reality... 阅读全帖 |
|
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h******y
发帖数: 1374 | 36 The Nobel Prize in Physiology or Medicine 2011
shall be divided, with one half jointly to
Bruce A. Beutler and Jules A. Hoffmann
for their discoveries concerning the activation of innate immunity
and the other half to
Ralph M. Steinman
for his discovery of the dendritic cell and its role in adaptive immunity
Summary
This year's Nobel Laureates have revolutionized our understanding of the
immune system by discovering key principles for its activation.
Scientists have long been searching for the g... 阅读全帖 |
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b****r
发帖数: 17995 | 37 在果蝇里做出Toll的发育学功能的，业界公认似乎是是德国女 Volhard， 95年诺贝尔
奖.Toll 不是英语，是德语，我记得是 哇,了不起!的意思
我copy一段wiki
Mutants in the Toll gene were originally identified by 1995 Nobel Laureates
Christiane Nüsslein-Volhard and Eric Wieschaus and colleagues in the fruit
fly Drosophila melanogaster in 1985[2], and cloned by the laboratory of
Kathryn Anderson in 1988.[3] |
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b****r
发帖数: 17995 | 38 在果蝇里做出Toll的发育学功能的，业界公认似乎是是德国女 Volhard， 95年诺贝尔
奖.Toll 不是英语，是德语，我记得是 哇,了不起!的意思
我copy一段wiki
Mutants in the Toll gene were originally identified by 1995 Nobel Laureates
Christiane Nüsslein-Volhard and Eric Wieschaus and colleagues in the fruit
fly Drosophila melanogaster in 1985[2], and cloned by the laboratory of
Kathryn Anderson in 1988.[3] |
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y******8
发帖数: 1764 | 39 I am well aware of the famous evolution story of fly. However, unless human
or ancient apes could be down-sized by 10 fold and their life-cylces
accelerated by 10 fold, the severe chromosome abnormalities could drive the
species extinct when located in a competition-rich environment like south Africa.
In the lab, we can easily find fly strains with huge chromosome deletion/
duplication/inversion but viable and fertile. If the situation happened even
at 10% extent in human genome, the individual ... 阅读全帖 |
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c**c
发帖数: 11 | 40 我看他实验室网页的介绍，只剩下social behavior了。。
We are interested in the molecular and cellular mechanisms of social
behaviors.
Social behaviors are those initiated by one animal directed towards another
animal of the same species, ranging from social interactions in animals to
social relationships, politics and war in humans.
We use multidisciplinary approaches, integrating molecular biology, genetics
, neurobiology, chemistry, modern imaging and electrophysiology, to study
important and basic social behavio... 阅读全帖 |
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n***g
发帖数: 5027 | 41 The people working in ESC field are mostly from animal reproduction field
and they don't have good knowledge in genetic screening before 2006.
So Yamanaka's screening strategy is very outstanding in ESC field but maybe
not so good in drosophila or C-elegants field. |
|
|
D*a
发帖数: 6830 | 43 我喜欢这句话！
Moreover, Jules was very far from the actual‘bench work’. I think it fair
to say that he has always felt better among vertebrate immunologists than
Drosophila geneticists. |
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L*******e
发帖数: 2153 | 44 For a long time starvation/calories restriction has been considered as one
prominent way to slow down aging or increase longevity. : )
Increasing expression of sirtuin proteins has been indicated to extend
lifespan on models such as C. Elegans and Drosophila melanogaster.
However, lately there are some controversies on the effects of sir2 gene on
longevity . Some people question the genetic back-crossing artifacts on
those transgentic models maybe the direct cause of sirtuin's protective
effec... 阅读全帖 |
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h*******e
发帖数: 142 | 45 被选中做Platform Talk， 所以决定还是去了，虽然老板可能不掏钱。
故为了省钱，问问有谁也去，并且愿意share Hotel room 的？ 我愿意自带睡袋睡地板
。 多谢 |
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g******w
发帖数: 852 | 48 No1 ------------
Genomic imprinting in Drosophila has properties of both mammalian and insect imprinting.
Anaka M, Lynn A, McGinn P, Lloyd VK.
Dev Genes Evol. 2009 Feb;219(2):59-66. Epub 2008 Nov 25.
PMID: 19031081
[PubMed - indexed for MEDLINE]
No2---------------
Nat Rev Genet. 2007 Apr;8(4):272-85.
Transposable elements and the epigenetic regulation of the genome.
Slotkin RK, Martienssen R.
Source
Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA.
... 阅读全帖 |
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a***y
发帖数: 19743 | 49 请问，目前，为达到合理的coverage，Drosophila和mouse分别看，一个rep需要多少钱？
RNA-seq
Proteomics sequencing
都要是quantitative的，期望是能取代Microarray
谢谢！ |
|
g******w
发帖数: 852 | 50 paper help
--------------
Mohan, M., Herz, H.M., Smith, E.R., Zhang, Y., Jackson, J., Washburn, M.P.,
Florens, L., Eissenberg, J.C., Shilatifard, A. (2011). The COMPASS Family of
H3K4 Methylases in Drosophila. Mol. Cell. Biol. 31(21): 4310--4318.
Thank you very much in advance!
l************[email protected] |
|
