b*z
发帖数: 48 | 1 there are so many things that can go wrong in a pcr expt. your purpose would
be defined to carry out an expt without appreciable contamination rather
to figure out what has gone wrong.
typically if you want to find what's wrong, you would change one variables
(any buff, pcr station, even a pipette can be considered a single varibable)
at one time, repeat pcr expt and figure out what has gone wrong.
I would not do that though. you only have to figure out whether your own pcr
station has contamina | n*******e
发帖数: 27 | 2 biz is a master guy on pcr. i have a little to say. i have been using
tail DNA for pcr characterization of mice KO. my experience is that
u should separate all the reagents/pippets/pippetmen from commonly
used stuff. so, first of all, go to clean the pcr station, and make it
as clean as possible. remember, always NOT to bring template to the
station. also, get pcr buffer/ddH2O changed frequently if u question it.
primers should be stocked as 100uM, and u may only aliquot them for use.
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