n********k
发帖数: 2818 | 1 Hi, Folks:
Any of you has any good sources about Ki67 Antibody? We had a great one (
Sp6 from neomarker) but the
new lot doesn't work with mouse anymore for some reasons unknown. They have
a replacement (Ab4) but
doesn't work nearly as nice...
Thanks in advance,
Neverthink |
n********k
发帖数: 2818 | 2 ding:)
have
【在 n********k 的大作中提到】
: Hi, Folks:
: Any of you has any good sources about Ki67 Antibody? We had a great one (
: Sp6 from neomarker) but the
: new lot doesn't work with mouse anymore for some reasons unknown. They have
: a replacement (Ab4) but
: doesn't work nearly as nice...
: Thanks in advance,
: Neverthink
|
c****l
发帖数: 1086 | 3 http://www.ebiotrade.com/buyf/Novocastra/alphk.htm
We use a Rabbit anti-Ki67 from Novocastra. It works great. |
n********k
发帖数: 2818 | 4 thanks a bunch:))) so do you work with mouse? and tissue or cells? May I
have a protocol...
【在 c****l 的大作中提到】
: http://www.ebiotrade.com/buyf/Novocastra/alphk.htm
: We use a Rabbit anti-Ki67 from Novocastra. It works great.
|
c****l
发帖数: 1086 | 5 http://www.leica-microsystems.com/products/novocastra-reagents/primary-antibodies
Look up here. I cannot remember which one exactly
but we use Rabbit polyclonal anti-Ki67, I think they probably have only one
Rabbit.I can have a look tomorrow and let you know.
Regular IHC protocol,
Mouse brain, 4%PFA fixation O/N
5um paraffin section (or 12um Cryosection)
deparaffination
ph 6.0 Citric acid antigen retrieval 100c 10 min
1 ab 4c O/N
2 ab Rm 1-2hrs
any question, let me know |
a****d
发帖数: 1919 | 6 we use the Ki67 antibody from vector lab. It is also a sp6 clone.
http://www.vectorlabs.com/catalog.aspx?prodID=1648
we use this antibody for mouse tissue section and cultured neural progenitor
cell staining (1:200). |
n********k
发帖数: 2818 | 7 thanks again, that's very helpful
one
【在 c****l 的大作中提到】
: http://www.leica-microsystems.com/products/novocastra-reagents/primary-antibodies
: Look up here. I cannot remember which one exactly
: but we use Rabbit polyclonal anti-Ki67, I think they probably have only one
: Rabbit.I can have a look tomorrow and let you know.
: Regular IHC protocol,
: Mouse brain, 4%PFA fixation O/N
: 5um paraffin section (or 12um Cryosection)
: deparaffination
: ph 6.0 Citric acid antigen retrieval 100c 10 min
: 1 ab 4c O/N
|
n********k
发帖数: 2818 | 8 great and thanks a lot...it seems we are working on so similar stuff:)). One
other colleague has also suggested this one today...
progenitor
【在 a****d 的大作中提到】
: we use the Ki67 antibody from vector lab. It is also a sp6 clone.
: http://www.vectorlabs.com/catalog.aspx?prodID=1648
: we use this antibody for mouse tissue section and cultured neural progenitor
: cell staining (1:200).
|
|
n********k
发帖数: 2818 | 9 Hi, Have u ordered/used this Ab recently? I just talked to vector, SP6
clone from theirs also stopped working
with mouse recently...it seems the problem likely happened to every company
with SP6 clone...
progenitor
【在 a****d 的大作中提到】
: we use the Ki67 antibody from vector lab. It is also a sp6 clone.
: http://www.vectorlabs.com/catalog.aspx?prodID=1648
: we use this antibody for mouse tissue section and cultured neural progenitor
: cell staining (1:200).
|
s******e
发帖数: 370 | 10 novacastra, now belongs to leica |
n********k
发帖数: 2818 | 11 thanks, I am gonna try it...
【在 s******e 的大作中提到】
: novacastra, now belongs to leica
|
a****d
发帖数: 1919 | 12 You are right. The Vector Ki67 antibody gave us problem too. It looks like
we have to switch to another one.
company
【在 n********k 的大作中提到】
: Hi, Have u ordered/used this Ab recently? I just talked to vector, SP6
: clone from theirs also stopped working
: with mouse recently...it seems the problem likely happened to every company
: with SP6 clone...
:
: progenitor
|
T****u
发帖数: 424 | 13 thanks
【在 c****l 的大作中提到】
: http://www.leica-microsystems.com/products/novocastra-reagents/primary-antibodies
: Look up here. I cannot remember which one exactly
: but we use Rabbit polyclonal anti-Ki67, I think they probably have only one
: Rabbit.I can have a look tomorrow and let you know.
: Regular IHC protocol,
: Mouse brain, 4%PFA fixation O/N
: 5um paraffin section (or 12um Cryosection)
: deparaffination
: ph 6.0 Citric acid antigen retrieval 100c 10 min
: 1 ab 4c O/N
|
