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Biology版 - 请教一个beta-galactosidase组织染色的问题(paraffin embedded sections)
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谁给推荐个好的beta-Galactosidase antibody再请教个问题,切brain paraffin sections
再发一次 gene表达与否和histone modification关系多大?dapi和hoechst哪个更好用?
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[合集] What is the subcellular location?YFP为啥是green的
相关话题的讨论汇总
话题: paraffin话题: sections话题: embedded话题: 染色
进入Biology版参与讨论
1 (共1页)
l********1
发帖数: 54
1
老板非让做,没办法。但是在网上搜了下,beta-galactosidase staining一般染活细
胞或者冰冻组织。
我们手上只有paraffin embbeded的切片,有没有朋友做过这种片子的染色?有没有
protocol或者文献可以交流一下?
谢谢了。
H****N
发帖数: 997
2
there is no way to do b-gal staining with paraffin-embedded sections. The
only choice is to label b-gal with an antibody
l********1
发帖数: 54
3
Thanks! I'll try to stain p16, another marker for senescence.
p******6
发帖数: 410
4
跟贴同问一下,那些是比较公认的标准senescence marker,似乎SA-B-Gal是个金标准,
不用它的话p16, p21, HP1 gamma以及DAPI染出的heterochromatin foci能取代吗?
1 (共1页)
进入Biology版参与讨论
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YFP为啥是green的谁给推荐个好的beta-Galactosidase antibody
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Is there anyway to use Image J to score DAPI,please?DNA体积不到细胞核的1%
some nuclear dye not overlaP with FITC?[合集] What is the subcellular location?
ask a question关于microRNA的问题
求教做过His-tag protein purification的人问个技术问题。。frozen section 做in situ hybridization
paper help LEU2 directed expression of β-galactosidase activity and phleomycin resistance in Yarrow请教用7-AAD做viable marker
请教E.coli beta galactosidase 无活性突变体wnt 通路还有得做没?
相关话题的讨论汇总
话题: paraffin话题: sections话题: embedded话题: 染色