R****n
发帖数: 708 | 1 I'd like to analyze a DNA fragment from genomic DNA. No PCR, The length is
around 100-300bp. How can I selectively extract the DNA fragment from
genomic DNA.Thanks |
n***w
发帖数: 2405 | 2 sonication?
I don't know... |
R****n
发帖数: 708 | 3 I am thinking about randomly break the genomic DNA by multi DNase. Then use
a oligonucleotide probe to capture the target strand. But i am not sure if
there are other smarter way. |
s******s
发帖数: 13035 | 4 没更好的方法了。打碎了用biotin-RNA probe pull下来吧
use
【在 R****n 的大作中提到】
: I am thinking about randomly break the genomic DNA by multi DNase. Then use
: a oligonucleotide probe to capture the target strand. But i am not sure if
: there are other smarter way.
|
G***y
发帖数: 1082 | 5 2nd this.
【在 s******s 的大作中提到】
: 没更好的方法了。打碎了用biotin-RNA probe pull下来吧
:
: use
|
R****n
发帖数: 708 | 6 can you show me a protocol for this?
【在 s******s 的大作中提到】
: 没更好的方法了。打碎了用biotin-RNA probe pull下来吧
:
: use
|
