w****u 发帖数: 1078 | 1 最近试了几次,使用spin的方法,效果都不好,效率特别低(5%左右)。请问谁有
working protocol.多谢。 |
d*p 发帖数: 534 | 2 同学,咱们做的课题怎么相似啊,等我把文章发了再告诉你,嗬嗬! |
w****u 发帖数: 1078 | 3 protocol最好提前share一下,因为内容不相关,不会涉及到你的成果的。 |
y*********r 发帖数: 78 | 4 I had the same problem before with murine primary T cells. Probably
retrovirus is better. |
w****u 发帖数: 1078 | 5 I tried retrovirus for OVER expression on murine primary T cells, there are
>60% infection. So, if lentiviral shRNA are not good for infection. Is that
better to clone this into retrovirus vector? thanks |
z******e 发帖数: 3 | 6 对retrovirus转human oncogene一直有疑虑,怕不安全。 |
d*p 发帖数: 534 | |
w****u 发帖数: 1078 | 8 CD4+ T cells.Any new idea? |
d*p 发帖数: 534 | 9 我这方法在CD8上还行。 在CD4上面没效果,你试试retronectin吧。 |