b****y
发帖数: 81 | 1 不太熟悉这个方面。
有没有这方面的例子。如果有结构就更好了
多谢！ | A******y
发帖数: 2041 | 2 Most antibody is generated using peptide, in your case, it is probably
generated from phos-peptide. Are you detecting protein or just peptide? If
just peptide, using mass-spec is probably easier. | b****y
发帖数: 81 | 3 no. Just wondering whether there is any sucessful example of a antibody that
can recognize a phos-peptide.
Most antibody is generated using peptide, in your case, it is probably
generated from phos-peptide. Are you detecting protein or just peptide? If
just peptide, using mass-spec is probably easier.
【在 A******y 的大作中提到】
: Most antibody is generated using peptide, in your case, it is probably
: generated from phos-peptide. Are you detecting protein or just peptide? If
: just peptide, using mass-spec is probably easier.
| A******y
发帖数: 2041 | 4 A lot of antibodies for phos-"whatever protein" is generated from phos-
peptide as antigen, and the resulting antibody only recognize the phos-
peptide on the protein. It will also recognize the phos-peptide; therefore,
you can block the antibody binding using the peptide as competitive control
. You probably can do an ELISA if you want to see if the phos-peptide is
there or not. Btw, there are antibodies can even recognize a single
phosphorylated amino acid. Just go to any commercial antibody company sites
and type look for phosphorylated etc. |
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