w****s
发帖数: 235 | 1 大家都用什么cross linker to cross link peptide for antibody purification?
NHS agorase beads 好用么？ |
X***n
发帖数: 366 | 2 真有钱。理论上高碘酸酸氧化（sodium periodate)一下agarose，生成的醛基足够挂带
氨基的生物分子了。 |
c**n
发帖数: 73 | 3 pierce kit works very well for me
【在 w****s 的大作中提到】
: 大家都用什么cross linker to cross link peptide for antibody purification?
: NHS agorase beads 好用么？
|
w****s
发帖数: 235 | 4 I'm having a problem to yield sufficient antibody (rab polyclonal)from
the
1st serum bleed (~6ml serum).. 总共得到了100 ug.. but based on the ELISA,
the antibody
tilter is pretty high ~100k; my intuition is that I should get much
more. so I guess either I did not X- link well
the
peptide/antigen( should check with MS though) or simply low yield is
expected for 1st bleed?
有谁做过/有经验么？ 谢谢。 |
