y****6
发帖数: 196 | 1 I need to digest an antibody into small fragments. Which protease is the
best? Trypsin?
Thanks a lot |
k******0
发帖数: 1073 | 2 what is your purpose to analyse the small fragments? |
s******r
发帖数: 2876 | 3 木瓜蛋白酶。
【在 y****6 的大作中提到】
: I need to digest an antibody into small fragments. Which protease is the
: best? Trypsin?
: Thanks a lot
|
y****6
发帖数: 196 | 4 Thanks for sinister and kaka1000's reply. I labeled the antibody with
fluorescent dyes and quenchers, and I want to digest the antibody to small
fragments to break the FRET between dyes and quenchers. It would be best if
I can digest the antibody into the smallest fragments. Papain may not be the
best choice as it only cut antibody into three pieces. Maybe I can use some
kind of protease mixture? Any suggestion is welcome. |
k******0
发帖数: 1073 | 5 It is pretty hard to predict the proteolysis of a native protein and
therefore you have to try it out yourself by using different available
proteases or even a mixture if the proteases do not affect each other. |
b******y
发帖数: 627 | |
