F*Q
发帖数: 3259 | 1 从你的角度来理解， 你说得没错。也许是我的没说清楚，FtsZ的主要作用是pinch off
。对于budding 来说，pinch off 之前还有一系列的过程，比如说在什么时候bud开始
形成，长到什么时候bud就该pinch off，还有也许更重要的是为什么是bud而不是更普
遍的binary fission那种生长方式，我想知道是这些过程的调控机制的信息。 换句话说吧，哪个基因被敲除后bud就不长了，但细胞的其它进程不受影响。 |
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F*Q
发帖数: 3259 | 2 多谢你的信息！可惜，这些文献还是没有直接回答我想知道的问题。
我想知道那种确证了对细菌budding（而不是对bud从母体的分离）起调控作用的分子，
象我前面说的，那种分子的功能至少要包括决定某些细菌的增生是通过budding而不是
二分法或别的方式来实现。所以相关文献需要包含对该分子的机制研究，比如说敲除该
分子（基因）会导致细菌不长出bud（但是不影响细菌的其它生物属性）。
hallmark of bacteria ... cytokinesis and
. Filament structure of bacterial |
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f*******w
发帖数: 13821 | 3 不建议交易打印胖子；胖子是免费的，收费的是服务:
NBPR bud salty snack $7 mir form
出售/交换物品的名称:
NBPR bud salty snack $7 mir form
物品类别（coupon：mfc等；血糖仪等）:
al ar ct hi in ky ma me mn mo nc nj ny ri sd ut va wv
7/1-7/31, offer 30082
7baozi 3张以上6baozi
ensure tmf 5baozi
$99 bud grill，patio furniture，get $10 visa prepaid card
物品来源（报纸夹页，厂家邮寄等）:
可接受的价格（必须明码标价，必填）:
7baozi，3张以上6baozi
邮寄损失方式哪方承担（若需邮寄，必填）:
付款方式说明:
本贴有效期（必填）:
联系方式（例： 站内）: |
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h*******n
发帖数: 931 | 4 出售/交换物品的名称:
Bud $7/14 salty, etc.
Save $7 wyb salty snacks, ice, charcoal, propane, dips, condiments, buns, coolers, windshield wiper fluid, mulch, meat snacks, deli sandwiches, bottled water, and prepared foods (inc sandwiches, chicken wings/drums, or pizza). Purch price must exceed $14
Send form and dcrt(s) between 11/1/11 - 12/31/11
Offer # 30614
Goes to El Paso
NBPN Good in AL, AR, CT, HI, KY, MA, ME, MN, MO, NC, NJ, NY, RI, SD, UT, VA, and WV
all traded
Bud $6/10 turkey/ham/deli platter
Sav... 阅读全帖 |
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F*Q
发帖数: 3259 | 7 Thanks a lot for the list! Some of them may be related to bacterial budding.
However, I am not convinced that any of them is directly associated with
the molecular pathway (or regulators) of bacterial budding.
By the way, I am not working on bacterial budding or fission, not even close
. That's why I came here to ask. |
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c****x
发帖数: 2674 | 10 不建议交易打印胖子；胖子是免费的，收费的是服务:
出售/交换物品的名称:
$10 Bud Light Lime ~ Chicken Rebate - 06/30/10
物品类别（coupon：mfc等；血糖仪等）:
Rebate form
物品来源（报纸夹页，厂家邮寄等）:
Supermarket
Receive a $10.00 Prepaid Card via Mail-in Offer on the purchase of Fresh or
Frozen Chicken* with the purchase of TWO(2) 6-packs or ONE(1) 12-pack or
larger (cans or bottles) of Bud Light Lime
*Purchase price of Fresh or Frozen Chicken must exceed $10.00
Valid for residents of AK, AZ, CA, CO, DC, DE, FL, GA, IA, ID, IL, KS, MD,
MI, MS, MT, ND, NE, N |
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e**p
发帖数: 4259 | 11 Bud家有啥rebate啊，前几天刚买了一箱bud |
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h*******n
发帖数: 931 | 13 已经是第3波了，还有没有人需要的.
出售/交换物品的名称:
ud Light Lime $6 MIR for purchase to exceed $10 of Beach Towels/Mats, Beach
Hats, Sunglasses, Sandals, Sun Tan Lotions/Oils, Coolers or ANY other Summer
Pool or Beach Party Items. NBPR and valid for AL, AR, CT, HI, IN, KY, LA,
MA, ME, MN, MO, NC, NJ, NY, RI, SD, UT, VA and WV. recdeipt(s). Offer #30107
0.60/each
---------------------------------------------------
Bud Light $6 MIR for purchase to exceed $10 of Beach Towels/Mats, Beach Hats
, Sunglasses, Sandals, Sun ... 阅读全帖 |
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c**********n
发帖数: 13712 | 14 不建议交易打印胖子；胖子是免费的，收费的是服务:
出售/交换物品的名称:
Bud light Rebate $7 when purchase exceed $14
NBPR in AL,AR,CT,HI,KY,MA,ME,MN,MO,NC,NJ,NY,RI,SD,UT,WA,WV
expire 10/31/2011
物品类别（coupon：mfc等；血糖仪等）:
On purchase of salty snake, ice, charcal,propane,dips, condiments,buns,
cooler,windshield wiper fluid,mulch,meat snacks,deli sanwiches,bottled water
and prepared food(sandwiches,chickend wings/drums or pizza)
http://www.ebay.com/itm/7-00-Bud-Rebate-Form-10-31-11-salty-snk
物品来源（报纸夹页，厂家邮寄等）:
可接受的价格（必须明码标价，必填）:
5个包子+0.... 阅读全帖 |
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f*******w
发帖数: 13821 | 15 不建议交易打印胖子；胖子是免费的，收费的是服务:
Bud 家 $6 turkey, ham deli platter rebate form
出售/交换物品的名称:
Bud 家 $6 turkey, ham deli platter rebate form
物品类别（coupon：mfc等；血糖仪等）:
offer 30616 11/01-12/31
物品来源（报纸夹页，厂家邮寄等）:
可接受的价格（必须明码标价，必填）:
5baozi+shipping
邮寄损失方式哪方承担（若需邮寄，必填）:
付款方式说明:
本贴有效期（必填）:
联系方式（例： 站内）: |
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x**********3
发帖数: 1572 | 18 你的钱拿回来了吗？
我正打算从 Buds order 一把手枪。 好像如果在 Bud 用 credit card， 价格会自动
上升 3%， 你们为什什么不用银行转帐呢？ |
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s*******n
发帖数: 12995 | 19 不太可能是挑剩的。没有一个实体枪店能存它网上列出来的这么多的种类的枪支。实际
上它的网站枪的图样和解绍也不是他们提供的，而是有几个大的批发商提供的，buds自
己都没法改。buds只不过是拿订单再向批发商拿货。 |
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j****N
发帖数: 1289 | 20 质量问题找厂家。
只要没有transfer应该就可以退。但至少10% restocking fee。
我不是黑buds，但是下蛋前一定做好research，一旦下蛋，就算立刻cancel也要有10%
restocking fee。
另外推荐grabagun，价钱一般比buds略便宜，而且是CC price。shipping也快一些（基
于本人经验）班上大家不用估计是因为grabagun是在texas。
还有就是一定要check FFL受多少fee。便宜的firearm例如mossberg 500或 22lr 一定
是LGS划算。 |
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j****N
发帖数: 1289 | 21 我猜是吧。
我和buds和grabagun都deal过。都没问题。只是buds要慢一些。 |
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d*****n
发帖数: 908 | 22 我没有在auction的地方买过枪，不知gunbroker一类地方是否可靠？购买过程与Bud比
是否很麻烦？一直在等Bud的Benelli M2，可一直没有。有点等不及了。gunbroker倒是
有，但没买过，不知那里的店家信誉如何？
那里现在倒是没有exactly我要的。我想要的是Benelli M2 Tactical Pistol Grip
Ghost Ring，现在有货的，都只有Pistol Grip或Ghost Ring，没有二者都有的。
有朋友和我说过，找有“buy now”的。如果没有这个，是不是这个店家级别不够？ |
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j*****v
发帖数: 7717 | 23 bud真的好黑，grabagun没货了它就开始涨
［在 webhero (网侠) 的大作中提到：］
:bud涨价真是闪电战速度...
[发自未名空间iPhone版] |
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f********r
发帖数: 3155 | 24 你这是因祸得福了，因为buds经常以次充好，这里就有人买来的品相很差
Big 5打折后的Rossi只要$430，比buds便宜吧？ |
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q***x
发帖数: 94 | 25 Many new Chinese gun buyers are waking up after recent Houston tragedy and
are looking for the first reliable HD weapon, I just found out this great
deal yesterday and I highly recommend it after I confirmed some critical
details today.
brand new, life time warranty, sig 229 with night sights, 9MM, 15rds
capacity, $650 shipped
http://www.budsgunshop.com/catalog/product_info.php/cPath/21_28
(deal found from slickguns.com or gunwatcher.com, bookmark them)
cheapthandirt also has the same deal. But... 阅读全帖 |
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m****g
发帖数: 530 | 26 哥哥姐姐们，
小弟想知道在budding yeast kinome (122 kinases)又多少是essential的， 但是差了
半天， 只有fission yeast的，没有budding yeast 的， 感觉上不太可能， 不知那位
知道这方面的信息， 给片文献？
多谢 |
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F*Q
发帖数: 3259 | 27 大多数细菌是通过binary fission来产生新个体的，其中对fission起主要作用的一个蛋白质叫FtsZ。同时有些细菌(alpha-proteobactria)是通过budding来产生新个体的，我想知道是哪个或哪些蛋白或基因对budding起主导作用。有人知道相关信息吗？
切题的回复有包子答谢 |
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F*Q
发帖数: 3259 | 28 辛苦你了，发了这么多连接！虽然这些和baterial budding的调制机制没有什么联系（
根据我的调研，好像目前还没有人做过bacterial budding机制研究的，所以regulator
还没人知道），你助人为乐的精神还是值得鼓励的。等我有时间给你转一个包子吧（包
子不多，还得留着点以备需要）。 |
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F*Q
发帖数: 3259 | 29 在我更新的帖子里特意提到了FtsZ，那是binary fission的主要调控基因，但是没有证
据表明FtsZ也是budding的调控因子。我没查到专门研究bacterial budding机制的工作
。也许是太偏而无人问津吧，如果是我漏了，希望版上有人知道。 |
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I***a
发帖数: 13467 | 30 Caulobacter是你说的budding细菌之一，
差不多就是用Ecoli那一套东西。
挺有意思的，
2分裂菌用的东西，难道budding的就不能用？
只不过细节有点差别而已。
有多少细菌能逃脱fts,min,par 这几个系统， |
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l**********1
发帖数: 5204 | 31 //www.ncbi.nlm.nih.gov/pubmed/21050804
浮黴菌門
Isosphaera屬
//hamap.expasy.org/proteomes/ISOPI.html
Species: Isosphaera pallida (strain ATCC 43644 / DSM 9630 / IS1B)
Species code: ISOPI
Taxonomy: Bacteria; Planctomycetes; Planctomycetia; Planctomycetales; Planctomycetaceae; Isosphaera
(TaxID: 575540) [NEWT/ NCBI]
Description: Isosphaera pallida strain IS1B was isolated from a hot spring in Kah-nee-tah, Oregon, USA.
It is the only budding bacterium known to glide and the only heterotrophic bacteriu... 阅读全帖 |
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w*******y
发帖数: 60932 | 33 Thought this deserves a thread of its own. Sally's has these really cute
Plugged In Dazzle Ear Buds for $1.99 with free shipping. There is a $5 off
$5 or more code out there( LMART) that would make 3 of these .97 + Tax Total
shipped!! Even if you do not use the code $1.99 is still good.
$5 CODE: 555658
Link:
http://www.sallybeauty.com/earphone-buds/SBS-680902,default,pd.html?cm_vc=SEARCH
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w*******y
发帖数: 60932 | 36 Eversave has the Maximo iM-590 iMetal Isolation earphones in black or white:
http://www.eversave.com/shopping/maximo
for $24 shipped. Today (Monday) they have a $3 promo with the code CYBER
bringing them down to $21 shipped. Good set of canal buds, reviewed well as
a solid sub $100 set of canal phones, and that is at a $60 pricepoint. The
cheapest they seem to be now is close to $50. Good reviews, some good mods
to increase isolation. The fabric based cord helps keep the microphonics at
bay. I... 阅读全帖 |
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c***3
发帖数: 251 | 39 不建议交易打印胖子；胖子是免费的，收费的是服务:
出售/交换物品的名称:
啤酒rebate form(Bud Light offer 28381)
物品类别（coupon：mfc等；血糖仪等）:
Purchases must occur between:10/01/10-10/31/10
SAVE:6.00 VIA MAIL-IN REBATE on the purchase of salty snacks,ice,
coolers,condiments,dips,bottled water,pizza,buns and prepared foods from the
deli(platters,sandwiches,chicken wings and drums,meat snack or cheeses).
Compliments of the Budweiser Family.
Purchase price of combined qualifying items must exceed $10.00.
No beer purchase required.
Valid for r... 阅读全帖 |
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h*******n
发帖数: 931 | 40 不建议交易打印胖子；胖子是免费的，收费的是服务:
Prefer to trade for the forms I am searching for
出售/交换物品的名称:
Bud Light $6 MIR for purchase to exceed $10 of Beach Towels/Mats, Beach Hats
, Sunglasses, Sandals, Sun Tan Lotions/Oils, Coolers or ANY other Summer
Pool or Beach Party Items. NBPR and valid for AL, AR, CT, HI, IN, KY, LA, MA
, ME, MN, MO, NC, NJ, NY, RI, SD, UT, VA and WV. recdeipt(s). Offer #30097,
5/1-7/31/11
物品类别（coupon：mfc等；血糖仪等）:
beer rebate form
可接受的价格（必须明码标价，必填）:
6包子/each or paypal
邮寄损失方式哪方承担（若需邮寄，必填）:... 阅读全帖 |
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c******t
发帖数: 1050 | 41 不建议交易打印胖子；胖子是免费的，收费的是服务:
所求物品名称:
Bud Beer $7 Rebate Expires 7/31/11(Offer 30082)
物品类别（coupon: mfc 等；血糖仪等）:
MIR(东部最好，因为怕时间来不及，谢谢）
Form states: Save $7.00 via mail in rebate on the purchase of salty snacks,
ice, charcoal, propane, dips, condiments, buns, coolers, windshield wiper
fluid, mulch, meat snacks, deli sandwiches, bottled water and prepared foods
(including sandwiches, chicken wings/drums or pizza). Compliments of the
Budweiser Family
Offer # 30082
NO beer purchase is required and this r... 阅读全帖 |
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m****i
发帖数: 2485 | 42 昨晚整理pantry，发现几瓶Bud Light啤酒，不知是什么时候的。标签上写着是“Born
on date:29JAN08 D/03"是08年生产的到11年到期？还是08年到期？ |
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m*********n
发帖数: 11525 | 43 update：form暂时已经分完了，我再去店里看看有没有，有的话再多揪几张来分。
不建议交易打印胖子；胖子是免费的，收费的是服务:
出售/交换物品的名称:
啤酒rebate form(Bud Light offer 28381)
物品类别（coupon：mfc等；血糖仪等）:
Purchases must occur between:10/01/10-10/31/10
SAVE:6.00 VIA MAIL-IN REBATE on the purchase of salty snacks,ice,
coolers,condiments,dips,bottled water,pizza,buns and prepared foods from the
deli(platters,sandwiches,chicken wings and drums,meat snack or cheeses).
Compliments of the Budweiser Family.
Purchase price of combined qualifying items must exceed $10.... 阅读全帖 |
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j******u
发帖数: 41683 | 44 ☆─────────────────────────────────────☆
marsmission (静心) 于 (Sat Oct 9 22:47:58 2010, 美东) 提到:
update：form暂时已经分完了，我再去店里看看有没有，有的话再多揪几张来分。
不建议交易打印胖子；胖子是免费的，收费的是服务:
出售/交换物品的名称:
啤酒rebate form(Bud Light offer 28381)
物品类别（coupon：mfc等；血糖仪等）:
Purchases must occur between:10/01/10-10/31/10
SAVE:6.00 VIA MAIL-IN REBATE on the purchase of salty snacks,ice,
coolers,condiments,dips,bottled water,pizza,buns and prepared foods from the
deli(platters,sandwiches,chicken wings and drums,meat snack or cheeses)... 阅读全帖 |
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c***3
发帖数: 251 | 45 不建议交易打印胖子；胖子是免费的，收费的是服务:
出售/交换物品的名称:
啤酒rebate form(Bud Light offer 28381)
物品类别（coupon：mfc等；血糖仪等）:
Purchases must occur between:10/01/10-10/31/10
SAVE:6.00 VIA MAIL-IN REBATE on the purchase of salty snacks,ice,
coolers,condiments,dips,bottled water,pizza,buns and prepared foods from the
deli(platters,sandwiches,chicken wings and drums,meat snack or cheeses).
Compliments of the Budweiser Family.
Purchase price of combined qualifying items must exceed $10.00.
No beer purchase required.
Valid for r... 阅读全帖 |
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c******g
发帖数: 694 | 46 FORM 简单的说就是~~
不用买啤酒只要买超过10块钱的salty snacks,ice,coolers,condiments,dips之类的，就
返给你6块
注意是receipt要在10/01/2010--10/31/2010的之间买的，
不建议交易打印胖子；胖子是免费的，收费的是服务:
出售/交换物品的名称:
啤酒rebate form(Bud Light offer 28381)
物品类别（coupon：mfc等；血糖仪等）:
Purchases must occur between:10/01/10-10/31/10
SAVE:6.00 VIA MAIL-IN REBATE on the purchase of salty snacks,ice,
coolers,condiments,dips,bottled water,pizza,buns and prepared foods from
the
deli(platters,sandwiches,chicken wings and drums,meat snack or cheeses).
Compliments of th... 阅读全帖 |
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h*******n
发帖数: 931 | 47 不建议交易打印胖子；胖子是免费的，收费的是服务:
Prefer to trade for the forms I am searching for
出售/交换物品的名称:
Bud Light $6 MIR for purchase to exceed $10 of Beach Towels/Mats, Beach Hats
, Sunglasses, Sandals, Sun Tan Lotions/Oils, Coolers or ANY other Summer
Pool or Beach Party Items. NBPR and valid for AL, AR, CT, HI, IN, KY, LA, MA
, ME, MN, MO, NC, NJ, NY, RI, SD, UT, VA and WV. recdeipt(s). Offer #30097,
5/1-7/31/11
物品类别（coupon：mfc等；血糖仪等）:
beer rebate form
可接受的价格（必须明码标价，必填）:
6包子/each or paypal
邮寄损失方式哪方承担（若需邮寄，必填）:... 阅读全帖 |
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h*******n
发帖数: 931 | 48 不建议交易打印胖子；胖子是免费的，收费的是服务:
物品名称:
bud light lime save $6 on the purchase of any fresh or frozen seafood,
purcahse occur 7/1-7/31
AR， MA,ME,MN,MO，NC,UT and VA only
想要交换 RA 3off15 code or pdf
物品类别（coupon: mfc 等；血糖仪等）:
form
物品来源（报纸夹页，厂家邮寄等）:
grocery store
可接受的价格（必须明码标价，必填）:
RA 3off15 code or pdf
邮寄损失方式哪方承担（若需邮寄，必填）:
付款方式说明:
本贴有效期（必填）:
till got
联系方式（例： 站内）:
站内 |
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h*******n
发帖数: 931 | 50 BUD ~ $7/$14 MIR on Salty snacks, Ice, Charcoal, Propane, Dips, Condiments,
Buns,
Coolers, Windshield wiper fluid, Mulch, Meat Snacks, Deli sandwiches,
bottled Water & prepared Foods
(including sandwich, chicken wings/drums or pizza) ~ NBPR ~
VALID: 7/1 - 7/31/11
STATES: AL, AR, CT, HI, IN, KY, MA, ME, MN, MO, NC, NJ, NY, RI, SD, UT, VA &
WV
OFFER: 30082
只换不转！ |
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