b*****e
发帖数: 288 | 1 08～09年，Cell Research发表了206篇文章，
同时间内，它的文章的被引用次数是1940。这其中有1925次引用来自于其他期刊。
如果用1940除以206，那么就是9.4
如果用1925除以206，那么就是9.3
既，把所有引用中来自于cell research的引用全部除去，完全是用其他刊物的引用次数计算影响因子，那么Cell Research的“他引影响因子”也有9.3
同理，PNAS的“他引影响因子”是9.440，因为PNAS的被引用次数中有3%是自引的。
明白？ |
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y***k
发帖数: 60 | 2 是的，以前cell research只有4分多的时候，上海生科院发cell research毕业算分数*
2，就是说两人co一篇cell research都能毕业...... |
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S****r
发帖数: 982 | 3 We performed a reporter assay on RAW cells days ago. The transfection
efficiency is fine, around 10-15% with EGFP. However, luciferase value is
extremely low, including both Firefly and Renilla that acts as the internal
control. I wonder if an activation is required for these cells, just like
handling in T cells that requires PMA and Ionomycin?
If it requires, what's concentration should I use?
Thanks in advance!
|
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t****t
发帖数: 45 | 4 Job Title: Postdoctoral Position in Biomaterials, Stem Cells, and Tissue
Regeneration
Application Deadline: Open Until Filled
Job Description
Description:
The Department of Biomedical Engineering at the University of Minnesota
currently has an opening for a post-doctoral fellow to conduct studies in
the area of biomaterials and tissue regeneration. The project involves
materials engineering on the molecular level, scaffold fabrication, and stem
cells.
Qualifications:
Candidates must have a Ph.D.... 阅读全帖 |
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t**u
发帖数: 7 | 5 帮朋友贴一个---
Postdoctoral position for Microcell Mediated Chromosome Transfer in Sloan
Kettering Institution, New York, NY.Department of Cancer Biology and
Genetics Center for Cell Engineering Sloan Kettering Institute, New York
Affiliated with Weill Medical College of Cornell University
Looking for individual who has a prior research experience on microcell
mediated chromosome transfer (MMCT).
The positions will focus on mechanistic approaches to understand 1)
reprogramming of somatic cells to plu... 阅读全帖 |
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a*******e
发帖数: 245 | 6 也许cell research的if比较搞，但是who cares
能发embo，肯定不会投cell research阿，俺觉得jbc 和jmb 的认可度都比这个cell
research 强多了 |
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q**********0
发帖数: 335 | 7 To evaluate a viral protein's cacinogenesis ability, we are using cell
transformation assay. which cell line is the best choice to do this type of
assay? Thanks. |
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f***4
发帖数: 886 | 8 高手们近来讨论一下体内cancer stem cell
究竟体内存在cancer stem cell吗？
如果存在的话，是不是如果有突变环境情况下，他们就能产生cancer
怎么证明某类细胞可能是cancer stem cell
纯外行，低水平问题。见谅。 |
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d*p
发帖数: 534 | 10 Tumour-infiltrating regulatory T cells stimulate mammary cancer metastasis
through RANKL–RANK signalling
Figure 2e.
This is the only evidence to show that the cells they are working on is Treg
. Unfortunately, FoxP3 in CD25+ only showed 2 folds change compare to CD25-.
I think they should say Activated T cells in stead of Treg. |
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p****z
发帖数: 31 | 11 问题1: 有人做过H1299的soft agar的growth 吗?用的是0.5% base agar+0.4% top
agar. 看paper上,都能做出来.我做了两次,都没有成功.实验方法与操作应该都没有问
题,同时做的H460长得很好.
问题2:soft agar上长出来的cell colony是cancer stem cell/tumor initiating cell
吗?
谢谢! |
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H*****e
发帖数: 120 | 12 1. H1299 needs 2-3 weeks for seeing colonies, whereas H460 colonies can be
seen in 5-7 day. If it still does not work, change the mediumn every 4 days
or order new cells from ATCC. We routinely do it and no problem found.
2.It has nothing to do with stem cells. Most stem cell medium are serum
free. But Soft agar uses 10-20% serum (I even saw people use 30% serum for
weak oncogene transformed 3T3.).
Good luck! |
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o******d
发帖数: 204 | 13 说到最后一点，其实对学生也不是坏事，刚发现这篇Cell的一个第一作者这是去年那篇
Nature Immunology的第一作者，在国内岂不是发大了？
他确实挺出色的。
06年回国，09年还是10年已经有了国内得pnas和自然免疫。
当然cell总是表示上了一个层次。
但是也不算特别惊讶，因为
（1）人家博后老板是beutler啊，大牛。文章送审不会被整太惨。
（2）免疫学科：从大牛实验室出来独立发文来看免疫得档次是最高的，从发育，神经
顶级大牛实验室出来很多发过cns得博后出来自己独立头5年一般就是10分以下水准，
developement,JNI，能发cell子刊级别得已经非常牛。独立5年内就能发cns或者nature
子刊得别说国内，国外top得ap
也很难。
然而免疫出来的几乎每个独立后都很快能发很好的文章。
参见flavel最近出来的中国博后，li ming (mskcc), chi hongbo(st jude), wan
yisong(nuc)，几乎都是3年内就有immunity或者自然免疫出炉。同等比较做发育（
elaine fuchs，schier为例），细胞（xia... 阅读全帖 |
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n*******n
发帖数: 515 | 14 版上高手众多，如果知道答案，不吝赐教。
我是做有机合成的，平时也会养一些细胞, 主要是cancer cell lines。
这次被老板指派了一个project需要primary neuron cells。这可从来没做过，试了几
次，可每次harvest neuron cells用trypsin或是scraping的话要不然下不来，要不然
百分之六七十的细胞已经挂了。。。
请有经验的同学指点一下吧，有包子。谢谢 |
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m********m
发帖数: 263 | 15 Cell press的 cell reports 据说focus on shorter, single-point story，不知道
具体要什么档次的文章才敢投？类似与Molecular Cell？ |
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m*********n
发帖数: 158 | 16 endogenous protein pulldown,以前都是harvest cell lysis，然后antibody incubation overnight, next day add
beads for 1-2hours, and pulldown
听说把beads先和antibody cross link一下，可以提高pulldown efficiency;
所以准备把antibody-bead cross link,加到cell lysis,这样得话，大家一般incubate
在cell lysis里面多久呀？
谢谢 |
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d*p
发帖数: 534 | 17 Idea is not new, but make it happen in human is very exciting. Clean 90%
cells in 4 hours. 24 hours, all active cells will be cleaned. The safety of
cell therapy will no longer be a big issue now. |
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n********k
发帖数: 2818 | 18 Are u talking about stem cell therapy in general or more about in immuno-
lineage? Seems to me, you are referring to the T cell or DC therapies etc..
.BTW, I have no experiences with cell therapy except from research point of
view...and I am not working in blood-related lineages either...
the
been
TCR,
won' |
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z*******2
发帖数: 658 | 19 各位老大：
能否给小弟启启蒙，老板要我做stem cell 请问 用stem cell 和IPS 的区别是什么那
个更有优势，如何培养，一定要加明胶和feeder cell 吗？那两种细胞不是混在一起了
吗 |
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C***Y
发帖数: 61 | 20 I usually add puromycin to cells 36 hrs after transduction,
and harvest cells for RNA 72 hrs after adding puromycin. You should be able
to get enough cells for microarray this way. Actually, I am testing inducible systems now,
and the problem I having now is leakage.
？ |
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b*******0
发帖数: 48 | 21 you can directly tell if the cell is in M phase by looking at the chromosome
arrangement. Cells in M phase tend to have no nuclear membrane and
condensed chromsomes are ready to be aligned at the plate.
A good marker for cells from last S to G2 phase is CEP-F antibody. |
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h*****G
发帖数: 113 | 23 human embryonic stem cell differentiated的细胞cell surface marker staining
需要block Fc receptor吗？
如果需要，一般怎么做？
谢谢。 |
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s******y
发帖数: 28562 | 24 This cell line was supposed to be a cardiomyocyte cell line.
However, I almost never saw anyone in paper using it. Does anyone know
anything about this cell line?
Thanks. |
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g*********5
发帖数: 2533 | 25 thanks for your advice, andy.
I don't know how to culture the primary cells.
and I want establish some rho-zero cells (delete the mtDNA)
and I need long time incubation the cell with EB.
so how many passages usual the primary RGC survive?
thanks. |
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M*****e
发帖数: 279 | 26 Paul Worley is a non-practicing MD. His lab found a small GTPase, Rheb, which was later found to be an important player in other signaling pathways, such as mTOR (insulin/Akt/mTOR/S6K) and TSC1/TSC2 (cell growth, cell cycle, cell size).
Reference: K. Yamagata, L.K. Sanders,W.E. Kaufmann,W. Yee, C.A. Barnes, D.
Nathans, P.F. Worley, J. Biol. Chem. 269 (1994) 16333– 16339.
http://cmm.jhu.edu/index.php?title=Paul_F._Worley |
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j***s
发帖数: 42 | 27 The following is a e-mail from the first author of Paul Worley Cell paper.
He says something like "publish anything below Neuron is unacceptable"...
This is crazy
---------- Forwarded message ----------
From: MUHAMMAD BANGASH
Date: Oct 28, 2006 10:44 AM
Subject: RE: ALumni with PhDs
Dear Saad,
Thank you for contacting me. I am sorry, due to repeated vilification/
impersonations, I do not subscribe to Topica.
It really depends on what you want to do.
If you want a PhD to augment ... 阅读全帖 |
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M*****e
发帖数: 279 | 28 Paul Worley is a non-practicing MD. His lab found a small GTPase, Rheb, which was later found to be an important player in other signaling pathways, such as mTOR (insulin/Akt/mTOR/S6K) and TSC1/TSC2 (cell growth, cell cycle, cell size).
Reference: K. Yamagata, L.K. Sanders,W.E. Kaufmann,W. Yee, C.A. Barnes, D.
Nathans, P.F. Worley, J. Biol. Chem. 269 (1994) 16333– 16339.
http://cmm.jhu.edu/index.php?title=Paul_F._Worley |
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j***s
发帖数: 42 | 29 The following is a e-mail from the first author of Paul Worley Cell paper.
He says something like "publish anything below Neuron is unacceptable"...
This is crazy
---------- Forwarded message ----------
From: MUHAMMAD BANGASH
Date: Oct 28, 2006 10:44 AM
Subject: RE: ALumni with PhDs
Dear Saad,
Thank you for contacting me. I am sorry, due to repeated vilification/
impersonations, I do not subscribe to Topica.
It really depends on what you want to do.
If you want a PhD to augment ... 阅读全帖 |
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h***e
发帖数: 232 | 30 i'm a new to this.
Just wondering if DAPI can get into live mammalian cells and stain the
nucleus? If yes, why many protocols require cell fixation and
permeabilization?
Will DAPI do anything to membrane protein if it stains live cells?
Many thanks! |
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n********k
发帖数: 2818 | 31 C2C12 is both a very easy and very hard line to work with and I am not aware
any other better established line other than this one or one could go with
primary lines...
Anyhow, If one follows closely certain routines, this line is very easy--
-passage before 80-90% confluence with no local overgrowth...Overgrowth is a
huge problem with this line, after 3-5 times, the entire culture will be
changed...Also, the confluence etc matters a lot for differentiation...
In addition, there are at lea... 阅读全帖 |
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l****j
发帖数: 70 | 33 我是分别从wt和ko mouse embryo 中分离的MEF cell,不是用来做feeder，
敲除的基因能在 ER stress，apoptosis，autophagy 中起作用，
我有overexpression的细胞系，但还想用MEF cell 做类似的实验，其他人发表的文章
用过MEF cell，我也想试试。 |
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m********m
发帖数: 263 | 34 这个问题和我的ID相关，所以粗谈鄙人的一点陋见。与normal cell 相比，为了满足其
快速生长，cancer cell是，大量利用所有可利用的资源，但是是极其浪费的。原因无
非是各种原因引起的代谢途径的紊乱，所以代谢紊乱也经常被发现于cancer cell 内。
未来可能的走向无外于通过对于某个失调或突变的代谢途径或酶的研究以希望能给癌症
治疗带来契机，但是代谢过程往往是牵一发而动全身，而其影响代谢的 原因也可能千
变万化，所以道路是曲折的，但是混口饭吃还是可以的。 |
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m********m
发帖数: 263 | 35 这个问题和我的ID相关，所以粗谈鄙人的一点陋见。与normal cell 相比，为了满足其
快速生长，cancer cell是，大量利用所有可利用的资源，但是是极其浪费的。原因无
非是各种原因引起的代谢途径的紊乱，所以代谢紊乱也经常被发现于cancer cell 内。
未来可能的走向无外于通过对于某个失调或突变的代谢途径或酶的研究以希望能给癌症
治疗带来契机，但是代谢过程往往是牵一发而动全身，而其影响代谢的 原因也可能千
变万化，所以道路是曲折的，但是混口饭吃还是可以的。 |
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z*t
发帖数: 863 | 36 在做相关项目，上来胡说几句，当是抛砖引玉，大家多多指正
otto warburg老早就发现cancer更喜欢glycolysis，还因此得了诺奖，之前的肿瘤代谢
主要在体外造影上有一定的应用，为啥肿瘤代谢要到现在又火了呢。在这个field里真
正让代谢又火起来的个人看来是三个topic: PKM2, IDH1/2, Lkb1/mTor/AMPK。
尤其是Lewis Canteley 2008 Pkm2的nautre，让人们知道了cancer cell是如何建立
Warbug
effect的，这之后各种可能和warburg effect相关的pathway又被人们陆续挖掘了出来
。而通过对PKM2/LDHB等glycolysis相关酶的knockdown, tumor cell的生长也确实受到
了影响。
这个领域重新开始火的另外一个原因是在2000-2010这十年间组学概念的热潮，催生了
代谢组这个概念。现在用GC/LC-MS进行代谢组的测定已经成为和microarray测转录一样
的技术了，肿瘤代谢的第二个重磅炸弹是Bert Vogelstein发现在glioma里有相当高比
例的ID... 阅读全帖 |
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z*t
发帖数: 863 | 37 在做相关项目，上来胡说几句，当是抛砖引玉，大家多多指正
otto warburg老早就发现cancer更喜欢glycolysis，还因此得了诺奖，之前的肿瘤代谢
主要在体外造影上有一定的应用，为啥肿瘤代谢要到现在又火了呢。在这个field里真
正让代谢又火起来的个人看来是三个topic: PKM2, IDH1/2, Lkb1/mTor/AMPK。
尤其是Lewis Canteley 2008 Pkm2的nautre，让人们知道了cancer cell是如何建立
Warbug
effect的，这之后各种可能和warburg effect相关的pathway又被人们陆续挖掘了出来
。而通过对PKM2/LDHB等glycolysis相关酶的knockdown, tumor cell的生长也确实受到
了影响。
这个领域重新开始火的另外一个原因是在2000-2010这十年间组学概念的热潮，催生了
代谢组这个概念。现在用GC/LC-MS进行代谢组的测定已经成为和microarray测转录一样
的技术了，肿瘤代谢的第二个重磅炸弹是Bert Vogelstein发现在glioma里有相当高比
例的ID... 阅读全帖 |
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l****j
发帖数: 70 | 38 看到Bio-rad有Criterion Cell（Vertical midi-format electrophoresis cell），
cat:165-6001,可以loading 12+2well,18well,26well，但是他们只有Criterion Empty
Cassettes卖，没有配套的glass plate
有其他公司的Midi-format electrophoresis cell带配套的glass plate吗？ |
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w********r
发帖数: 1431 | 39 有的。有几片研究小鼠的muscle stem cell/satellite cell Asymmetric cell
division的好文章 |
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c********r
发帖数: 1125 | 40
说对了，Frank Uhlmann是做cohesin领域里的中坚实力分子，算个小学霸级别了。
另一篇作者不是这个领域的，虽然工作量大，能发到molecular cell已经不错了。
小圈子很严重的，我原来国内实验室的一个工作，数据很有趣，但是完全不是我们固有
的领域，要抗体，合作都很碰壁，后来投了development被review各种狂轰滥炸，最后
发在cell res上。
结果几个月后同样分子的工作发在dev cell上，工作量比我们大一点点，但基本结论80
％都一样。
没办法，人家是这个领域的院士老怪实验室出来的，我们就一外来户。。。。 |
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a*****n
发帖数: 2835 | 42 I am using BECKMAN VI-CELL SERIES COUNTER, which can only take 50 pictures
and analyze the cells in these pictures. I wonder how to analyze more cells
using this machine? Thank you very much for your suggestion. |
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f*********6
发帖数: 94 | 43 You are the most welcome.
I only used the ERK inhibitor, and that was several years ago. The inhibitor
did improve the derivation efficiency. Having the p38 inhibitor in the
medium might help. You may also want to be sure that LIF is working well,
and i think this is very important.
The most tricky part to me is the patience. If you don't see any colony at
the first a few days, don't give up and just wait for a few more days.
Meanwhile set up more crossing in case that batch does not work, and t... 阅读全帖 |
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f*********6
发帖数: 94 | 44 Epiblast is a loose term to define undifferentiated pluripotent stem cells
in ICM.
Here is an excerpt from the review I suggested you to read:
...It is expressed in the 8- to 16-cell morula but becomes
restricted to the epiblast cells of the ICM in the expanded
blastocyst, and has been shown to be under the direct regulation of
the pluripotency factors Oct4 (Pou5f1) and Sox2 (Yuan et al.,
1995).
I will strongly suggest you to read the review if you have not done so. It
is a very well-written rev... 阅读全帖 |
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g*r
发帖数: 2116 | 45 反正2012年的时候 cell系列的rejection letter都默认带着cell reports的广告 |
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m********m
发帖数: 263 | 46 最近做实验看到cell morphology 有明显的改变，请教这里做 cell morphology 的牛
人，控制cell morphology的genes有那些？或推荐些review or research papers？ 谢
谢大牛！ |
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l**********1
发帖数: 5204 | 47 if cancer cell
'sox9'
pls refer
PMID 23201164
by Kopp JL et al.
Identification of Sox9-dependent acinar-to-ductal reprogramming as the
principal mechanism for initiation of pancreatic ductal adenocarcinoma.
(2012).
Cancer Cell. 22: 737-50.
PDF link:
HttP: //cmm.ucsd.edu/sander/sander/Home_files/Kopp_CancerCell_2012.pdf
>>
>>>
发信人: wdgca (全是骗银地), 信区: Biology
标 题: Re: genes in controlling cell morphology
发信站: BBS 未名空间站 (Wed Apr 10 16:44:27 2013, 美东)
做了啥treat，用的啥细胞都不说，那就木法猜。
> |
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m****b
发帖数: 32 | 48 我只说几句，说多了就有人知道我谁了。
胰岛素拮抗情况下， 本身就有 beta cell hyperplasia，解决不了问题，但是beta
cell 增生后寿命会缩短，成为beta cell failure，过分促进增生，细胞功能很加快丧
失，而加快糖尿病进程。 |
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m****b
发帖数: 32 | 49 我只说几句，说多了就有人知道我谁了。
胰岛素拮抗情况下， 本身就有 beta cell hyperplasia，解决不了问题，但是beta
cell 增生后寿命会缩短，成为beta cell failure，过分促进增生，细胞功能很加快丧
失，而加快糖尿病进程。 |
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d*p
发帖数: 534 | 50 Beads are much smaller than T cells. 1:1 ratio is good enough to activate T
cells. I suggest you learn what T cell synapse is, if you don't have
immunology background. |
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