w*********g
发帖数: 30882 | 1 倒霉蛋为什么总是无好运？ 读完此文可改变人生
译者：linuxor
发布：2013-03-28 01:23:29挑错 | 查看译者版本 | 收藏本文
不管是工作还是私生活，有些人顺顺当当幸福美满，而有些人不管干什么总是麻烦不断
厄运缠身。同样是人，差别咋就这么大呢。请新锐研究者藤井聪教授从心理学的角度来
给大家解说吧。
越是关照他人利益的人越走运
看看您的周围是否有这种现象？有些人不管是工作还是私生活中，不知为何总能左右逢
源一帆风顺。相反地，有些人像是命运女神的弃儿，无论做什么事情都不顺利，越活越
出溜。那么，为什么有人幸运、有福、运势强劲，有人倒霉、悲催、厄运连连？这两种
人究竟有何不同呢？
我们提出“认知焦点化理论”来解释这个问题。这是一个以人们心底里究竟最看重什么
为切入点进行的心理学研究。
简单而言，就是从人们在待人处事时能够从多大程度上关照他人情况这个观点出发，我
们尝试对人进行分类。
请看图1。横轴表示社会和心理的距离，以自我为原点，依次顺序为家人・恋人
→朋友→熟人→陌生人……越往右关系越远。而纵轴是时间轴，表示在待人处事时思虑
所及的时间跨度。是“只顾眼... 阅读全帖 |
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a***e
发帖数: 1010 | 2 you can download the cosmid sequence to your computer, and then according
to the position of tag-24 on that cosmid, you can select out the sequence
you need.
however, I never did that way. I prefer wormbase.
give
based |
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a*e
发帖数: 431 | 3 No offense; but the previous article that you forwarded makes no sense
whatsoever to me. If you are serious about this subject, research work
by Buss, Toody, and Cosmides can be much more informative.
David Buss:
http://homepage.psy.utexas.edu/homepage/Group/BussLAB/
Toody and Cosmides:
http://www.psych.ucsb.edu/research/cep/
The approach they took may not be the best way to think about social
evolutionary phenomena-they have been challenged all the time. As most of
us received some basic trai |
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S*******y
发帖数: 575 | 4 俺们没有啊！没准可以试试你们学校的，推荐下？fosmid基本跟cosmid一样，copy数低
，第一次用的是mySeq平台2x250 bp。 |
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S*******y
发帖数: 575 | 5 很奇怪，cosmid的NGS测序很难找到公司做（genome就很平常）我之前好像也发过帖子
问的，已经找了很长时间了，才找到这家公司的。确实，按理说应该换公司了，但是一
来他们熟悉情况了，没准trouble-shooting可以简约点时间，二来我确实苦于找到别的
好的地方。联系过几家core facility，反馈也不是很positive。
仁兄，有推荐的吗？我还会有很多这样的测序。 |
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s*****l
发帖数: 7106 | 6 寻找合作伙伴
我老婆在美国San Diego的一家生物公司工作，托我在DC附近帮她寻找代理商或者经
销商帮忙开拓市场。她们公司有较完整的核酸纯化体系，全球五家之一的病毒纯化技术
，还有重组蛋白、抗体等试剂，性价比较高。她们公司现在
的市场还主要在美国西部，客户分布在UC Berkley, Davis, Riverside, San Diego,
Caltech, USC, UC Irvine, City of Hope Cancer Research Institute ,The Scripps
research Institute, Burnham Research Institute,US Department of Agriculture
等。
主要产品有：
1) plasmid purification kits (including Plasmind mini 、midi 、maxi、mega
、96-well、endofree-plasmid、BAC, PAC, cosmid and P1 isolation、magnetic
beads plasmid isolat |
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s*****l
发帖数: 7106 | 7 寻找合作伙伴
我老婆在美国San Diego的一家生物公司工作，托我在DC附近帮她寻找代理商或者经
销商帮忙开拓市场。她们公司有较完整的核酸纯化体系，全球五家之一的病毒纯化技术
，还有重组蛋白、抗体等试剂，性价比较高。她们公司现在
的市场还主要在美国西部，客户分布在UC Berkley, Davis, Riverside, San Diego,
Caltech, USC, UC Irvine, City of Hope Cancer Research Institute ,The Scripps
research Institute, Burnham Research Institute,US Department of Agriculture
等。
主要产品有：
1) plasmid purification kits (including Plasmind mini 、midi 、maxi、mega
、96-well、endofree-plasmid、BAC, PAC, cosmid and P1 isolation、magnetic
beads plasmid isolat |
|
s*****l
发帖数: 7106 | 8 我老婆在美国San Diego的一家生物公司工作，托我在DC附近帮她寻找代理商或者经销
商帮忙开拓市场。她们公司有较完整的核酸纯化体系，全球五家之一的病毒纯化技术，
还有重组蛋白、抗体等试剂，性价比较高。她们公司现在
的市场还主要在美国西部，客户分布在UC Berkley, Davis, Riverside, San Diego,
Caltech, USC, UC Irvine, City of Hope Cancer Research Institute ,The Scripps
research Institute, Burnham Research Institute,US Department of Agriculture
等。
主要产品有：
1) plasmid purification kits (including Plasmind mini 、midi 、maxi、mega
、96-well、endofree-plasmid、BAC, PAC, cosmid and P1 isolation、magnetic
beads plasmid isolation 、ye |
|
s*********a
发帖数: 801 | 9 所有的灵长类都有24对染色体，只有人有23对，为什么？
http://en.wikipedia.org/wiki/Chromosome_2_(human)
All members of Hominidae except humans have 24 pairs of chromosomes. Humans
have only 23 pairs of chromosomes. Human chromosome 2 is widely accepted to
be a result of an end-to-end fusion of two ancestral chromosomes.[3][4]
Fusion of ancestral chromosomes left distinctive remnants of telomeres, and
a vestigial centromereThe evidence for this includes:
The correspondence of chromosome 2 to two ape chromosomes. The close... 阅读全帖 |
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J*****3
发帖数: 4298 | 10 http://en.wikipedia.org/wiki/Chromosome_2_%28human%29
All members of Hominidae except humans have 24 pairs of chromosomes. Humans
have only 23 pairs of chromosomes. Human chromosome 2 is widely accepted to
be a result of an end-to-end fusion of two ancestral chromosomes.[3][4]
Fusion of ancestral chromosomes left distinctive remnants of telomeres, and
a vestigial centromere
The evidence for this includes:
The correspondence of chromosome 2 to two ape chromosomes. The closest
human relative, ... 阅读全帖 |
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c*****t
发帖数: 1879 | 11 I don't have the protocol w/ me, but I believe that you can look
it up in the journal Biotechniques. It is a quite reliable method
which is basically:
1. get a set of degenerate primers, usually designed specifically
for your organism.
2. design a specific primer which binds to one end of your DNA.
3. do 3 rounds of PCR, 3 minute elongation time, 10 minute extention time.
i) very low anealing temperature (35C?) for 25 cycles. take 1 ul,
do a 1:20 (or 1:100?) dilution and |
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s***m
发帖数: 6197 | 12 是不是太大了DNA容易被shear？
一般这么大的片段不是用cosmid或者fosmid吗？ |
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k*****o
发帖数: 1486 | 13 Size doesn't matter, cos a regular cosmid/fosmid would be over 30 kb. Sorry
that I don't know anything about "Cre-ER construct", but I think it's the
protein that you want to express really matters. But not the size. |
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C*******e
发帖数: 4348 | 14 Invitrogen 的DH10B是适合做大Plasmid还有cosmid的
不好的是本身有Sp抗性
for |
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n********k
发帖数: 2818 | 15 thank you!!
Question1: So large plasmid always behave this way or not? the biggest one I
worked was 20kb, it was ok with typical condition.
Question2: so cosmids or Yac/Bac don't follow the same way as they have
multiple rep origisns, right?
background
the
also
days
plasmids. |
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r****r
发帖数: 36 | 16 1. It depends on what genes were in your plasmids. for example, it is ok to
manipulate some DNA viruses with large genome using conventional cloning
method but when it comes to some RNA viruses, you can't even clone its
fragments more than 3K.
2. I have no experience on Cosmid/YAC/BAC.
I |
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