s********e 发帖数: 2 | 1 study a drug,found cell cycle arrest in either G1 or G2/M (different cell
lines). also,E2F1 was downregulated ( western blot for both cell lines)。
can I say cell cycle arrest comes from E2F1 downregulated?
有点迷惑,求助!多谢,多谢! |
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B******f 发帖数: 2489 | 2 也许不够确切吧。
如果你再re-introduce E2F1, 在为drug未处理对照组有相似E2F1 level情况下,可以
rescue或部分rescue drug引起的cell cycle arrest, 应该更有说服力。 |
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s********e 发帖数: 2 | 3 多谢Bierhoff和happylqb2258的回复. 接着问问题.
如果同时看到Rb也被downregulated,和E2F1downregulation有关吗? Rb好像不是E2F1的
下游基因.多谢指点! |
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O******e 发帖数: 4845 | 4 ☆─────────────────────────────────────☆
ihavenothing (smallpotato) 于 (Fri Dec 15 16:47:35 2006) 提到:
同学们,请看这篇文章
Nature 435, 839-843 (9 June 2005)
c-Myc-regulated microRNAs modulate E2F1 expression
Kathryn A. O'Donnell1,2, Erik A. Wentzel2, Karen I. Zeller3, Chi V. Dang1,2,
3,5 and Joshua T. Mendell1,2,4
http://www.lib.ncsu.edu:2118/nature/journal/v435/n7043/full/nature03677.html
在Figure4a, 如果我没有理解错的话,这是要to determine whether E2F1 is a
target of miR-17-5p and miR-20a. 如果我没有理解错的话,正确的做法是,将E2F1
的 |
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h**********8 发帖数: 650 | 5 at least corelate with that
cell
。 |
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d*******s 发帖数: 61 | 6 看过该网站回复人的帖子后,发现原来造假问题是异常严重的:WB里面,GAPDH的一张
图片被反复用在多个图中;而且在他们后来发表的其它 8 篇论文中,还存在相同的问
题!这是很明显的蓄意作假了。这些论文在下面用黑体标明:
7 Responses:
Well that was easy….In the v282#17p12363 paper, look at the GAPDH bands in
Figure 3A. Now go back to Figure 2C and look at the GAPDH bands. Lanes 4-5-6
are the same. They duplicated the blot.In the v283#29p20045 paper, Fig 3A
right panel GAPDH blot contains the same 2 bands as Fig. 5D.So, the “
mistake” appears to involve using the same GAPDH blot for different
experiments. ... 阅读全帖 |
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