g*********3
发帖数: 177 | 1 大家好。
最近一段时间在挣扎gateway LR reaction:
用的是pLXSH gateway 作为destination ector
用的另一个entry ector含有attL1 ATTL2
所用的质粒测序无误含有recombination site。
用的是invitrogen的LR cronase enzyme mix II.
pLXSH is resistant to Amp and After LR reaction, I do transformation. I didn
't get any colony. [Zero.]
Entry vector is amp resistant too. So I cut the amp out and linearize this
vector.
I also do LR reaction with plxsh + Gus. Gus is positive control entry vector
provided by the kit.
There is no colony.
I have done positive p... 阅读全帖 |
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c*****t
发帖数: 520 | 2 Perhaps it is a foolish question.
I think that some types of Schrodinger equations and reaction-diffusion
equations are similar.
Semilinear Schrodinger equation: iu_t=u_xx+f(u).
Semilinear reaction-diffusion equation: u_t=u_xx+f(u).
But the methods for them are quite different. For example, for reaction-
diffusion equations, people ofen study the long time behavior of the
solutions by constructing subsolutions and/or supersolutions and using
comparison principle. For Schrodinger equations, perh... 阅读全帖 |
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w*******y
发帖数: 60932 | 3 Kenneth Cole Reaction Sunglasses With Purple Metal Frame and 100% UV
Protection!
List Price: $99.99
Savings: (92%) -$92.00
Our Price: $7.99
This pair of Kenneth Cole Reaction sunglasses is an elegant design that is
perfect for eye protection against harmful sun rays. Every Kenneth Cole
Reaction Fashion Sunglasses is brand new, never worn, with original factory
parts and 100% authentic guaranteed.
Features Include:
oPurple metal frame
o100% UV protection
oScratch and impact resistant lenses
Packa... 阅读全帖 |
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A******r
发帖数: 974 | 4 http://www.weather.com/travel/nightmares-fear-factory-terrified
Terrified tourists react during a 15-minute, pitch-black tour through the ‘
Nightmares Fear Factory’ haunted house in Niagara Falls, Canada. (
NightmaresFearFactory.com)
Show Thumbnails
October. A chill creeps into the air as the days edge closer to winter. The
leaves complete the change from green to red to brown. Children - and more
than a few adults - gear up for Halloween. And for those given to seeking
out frights this season, ... 阅读全帖 |
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u*******g
发帖数: 3300 | 5 Russian troop deployment in Ukraine's Crimean Peninsula is likely to trigger
a reaction from Turkey. Crimea lies only 173 miles from the Anatolian
coastline, across the Black Sea. It is home to a community of Turkic Tatars,
who are ethnic and linguistic kin of Anatolian Turks and oppose potential
Russian annexation of the peninsula. Turkey has established close ties with
Ukraine, a useful buffer with the bear to the north, since that country's
independence and will take issue with violation of K... 阅读全帖 |
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F*V
发帖数: 3978 | 6 Russian troop deployment in Ukraine's Crimean Peninsula is likely to trigger
a reaction from Turkey. Crimea lies only 173 miles from the Anatolian
coastline, across the Black Sea. It is home to a community of Turkic Tatars,
who are ethnic and linguistic kin of Anatolian Turks and oppose potential
Russian annexation of the peninsula. Turkey has established close ties with
Ukraine, a useful buffer with the bear to the north, since that country's
independence and will take issue with violation of K... 阅读全帖 |
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F*V
发帖数: 3978 | 7 Russian troop deployment in Ukraine's Crimean Peninsula is likely to trigger
a reaction from Turkey. Crimea lies only 173 miles from the Anatolian
coastline, across the Black Sea. It is home to a community of Turkic Tatars,
who are ethnic and linguistic kin of Anatolian Turks and oppose potential
Russian annexation of the peninsula. Turkey has established close ties with
Ukraine, a useful buffer with the bear to the north, since that country's
independence and will take issue with violation of K... 阅读全帖 |
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p****u
发帖数: 239 | 8 Reactions - Shuli You
http://blogs.nature.com/thescepticalchymist/2011/06/reactions_s
Shuli You is a Professor of Organic Chemistry in the State Key Laboratory of
Organometallic Chemistry, Shanghai Institute of Organic Chemistry, Chinese
Academy of Sciences, and works on methodology development, total synthesis,
and medicinal chemistry.
1. What made you want to be a chemist?
I was good at chemistry in high school and then chose chemistry as major in
university. Now I really enjoy being a chemist... 阅读全帖 |
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发帖数: 1 | 10 《来一碗Bii毕书尽》7.7生日快乐！！！参与节目活动有机会获得bii为你们准备的精
美礼品噢 赶紧行动起来吧
《来一碗毕书尽reaction 》和Bii一起追忆出道这7年的点点滴滴
【彩蛋】《来一碗Bii毕书尽Reaction》8分钟精华版 |
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l******h
发帖数: 395 | 11 【 以下文字转载自 shopping 讨论区 】
发信人: Wimbledonstr (南方铃), 信区: shopping
标 题: 有喜欢Kenneth Cole Reaction的吗？
发信站: BBS 未名空间站 (Wed Jul 7 18:44:56 2010, 美东)
请问：Kenneth Cole Reaction是个什么水平/名声的牌子？ |
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h****u
发帖数: 480 | 13 http://www.sfgate.com/cgi-bin/article.cgi?
f=/g/a/2011/05/21/businessinsider-apple-is-a-religion-neuroscientists-
find-it-triggers-the-same-reaction-in-your-brain-2011-5.DTL
The cult of Apple is real, according to neuroscientists.
They compared MRIs of Apple fans' brains to those of people who call
themselves "very religious" and found that Apple and religion light up
the same part of the brain. This means that Apple triggers the same
feelings and reactions in people as religion.
BBC highlights ... 阅读全帖 |
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b******s
发帖数: 1089 | 14 LR reaction sometimes gives odd outcome, such as no colonies or false
colonies. But mostly it should be very reliable.
You can try to gel purify both entry clone and destination clone and do LR
reaction immediately.
didn |
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y********g
发帖数: 24 | 15 今天被老板鄙视了,他说一个优秀的Organic chemist should know all name
reactions(他说起码400个),但是我最多know half of them.请教版上的有机大虾们是
不是都把name reaction的书从头背到脚的:) |
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R******i
发帖数: 33 | 16 I planned to synthesize two diketonates using Claisen Condensation reaction.
1, 2-acetothiophene+ethyl 2-thiophenecarboxylate
2, 2-acetylnaphthalene+ethyl2-naphathoate
use sodium methoxide as base and diethyl ether as solvent,acdify using HCl.
Question:
Reaction time (at RT)? Yield? How to promote the yield? How to purify the
product?
Thank you very much for your expert opinion. |
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m******3
发帖数: 983 | 17 Could anyone please recommend a website or book for summary for all
chemistry reactions?
Need to prepare national Olympiad chemistry and the test goes way beyond
general chemistry reactions.
BaoZi for Thanks |
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w********h
发帖数: 12367 | 18 http://pubs.acs.org/cen/coverstory/8051/8051olefin.html
December 23, 2002
Volume 80, Number 51
CENEAR 80 51 pp. 29-33
ISSN 0009-2347
OLEFIN METATHESIS: BIG-DEAL REACTION
A boon to organic synthetic chemists, olefin metathesis also promises cleaner,
cheaper, and more efficient industrial processes
A. MAUREEN ROUHI, C&EN WASHINGTON
Olefin metathesis is a popular and useful reaction. In the presence of certain
transition-metal compounds, including various metal carbenes, olefins exchange
the groups |
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c*****t
发帖数: 520 | 19 考虑reaction diffusion equation ：u_t=u_xx+f(u) ，x 定义域为实数集R ，t>0 。
假设f 是C^1 函数，以及在任意正时间T ，方程的解u(x,T)都属于H^1 空间。
若0 和1 为f 的零点（不排除f 还有其他零点），且令初值u(x,0)在0 和1 之间，则对
于任意x in R，t>0 ，解u(x,t)总在0 和1 之间。
我想知道解u(x,t)是否有如下性质：
若初值u(x,0)不是某个steady state（即u_xx+f(u)=0 的解），则对于任意0=
以及任意T>0 ，满足u(x,T)=A的x 值只有至多有限个。换言之，在任何正时间T ，方程
的解作为x-u 平面上的曲线，和直线u=A 只有至多有限个交点。
我的问题的动机，是我猜测reaction diffusion equation 如果不是初始即为steady
state ，那么在演化过程中，解不可能在某处严格“平坦”。即对于所有T>0 ，解在某
个区间(a,b) 上满足u(x,T)=A，或者对于某个收敛列{x_n} 满足u(x_n,T)=A，都是不可
... 阅读全帖 |
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s*****b
发帖数: 90 | 21 看了一本书， 讲Action Swing, Reaction Cycle什么的，不是太相信， 因为理论太对
称了，SPY2个月图，我就不太能找准什么地方是reaction cycle. 大家指点一下，不过
，这个当参考也行。 |
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发帖数: 1 | 22 【 以下文字转载自 USANews 讨论区 】
发信人: ThinkHarder (ThinkHarder), 信区: USANews
标 题: Is Andrew Yang Selling Out? Reaction to Yang Endorsing Bid
关键字: Andrew Yang,美国大选,候选人,访谈
发信站: BBS 未名空间站 (Sat Mar 14 08:07:44 2020, 美东) |
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g********d
发帖数: 4174 | 23 Reactions to NY Vote Felt Around the World
By Winston Gieseke
Across the country and around the globe, the news of New York’s
legalization of gay marriage dominated the wires. The impact of the Empire
State’s vote was felt in newspapers like the U.K. Telegraph, who predicted
that the news "will be the cause of huge celebrations in America at this
weekend's annual gay pride festivities in New York," as well as the Belfast
Telegraph, who labeled it "a breakthrough victory in the state where the
Am... 阅读全帖 |
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l****z
发帖数: 29846 | 24 By Jude Marfil
The Republican Party made official on Tuesday nomination of former Gov. Mitt
Romney as its candidate to challenge Democratic President Barack Obama on
Nov. 6, 2012. Tasked with bolstering the GOP candidate were his wife of 43
years, Ann Romney, who talked about his human side; and New Jersey Gov.
Chris Christie, who, in his keynote address, argued that his party’s ideas
“are right for America and their ideas have failed America.” Below are
reactions to Gov. Christie’s speech.
At ... 阅读全帖 |
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a*****h
发帖数: 201 | 25 【 以下文字转载自 Military 讨论区 】
发信人: ahmaswh (ahmaswh), 信区: Military
标 题: 笑死了: Shock reaction to the death of Margaret Thatcher
发信站: BBS 未名空间站 (Tue Apr 9 08:33:44 2013, 美东) |
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l**********n
发帖数: 60 | 26 Title:
Pediatric pharmacovigilance by medication antidote signals and comparative
evaluation of their sensitivity with routine techniques of ADR detection in
hospitalized children
Journal: BMC Pharmacology Toxicology
需要两个审稿人审稿，有pharmacovigilance, adverse drug reactions, medication
antidote signals 相关背景就可以了。
有兴趣请站内联系。 |
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s*****n
发帖数: 39 | 27 Surface enhanced Raman, Photoactive Yellow Protein, Modification/Reaction
请将姓名，工作单位，邮箱，以及一小段相关研究介绍，相关论文站内给我。 |
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c****1
发帖数: 5654 | 30 this still going on Volkswagen tour showed a new feature of TT action
reaction
time, for prof. players, smashing is about .2-.3 second, whereas looping is
about
.3-.5 close to tables.
guess our amateurs' would be around 1 second? |
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y*********n
发帖数: 763 | 31 一切按班上规矩来，3刀运费，paypal--personal--owe. 不退换，邮寄之后，责任归买
方。站内联系。
kenneth cole reaction down vest (60%down 40% feather)
穿上比较收腰，很服贴，不臃肿，本来准备带回国给自己老妈，和婆婆，结果一视频，
我妈嫌收腰，太年轻了。
size M
color one black one white one red,
借用一下恶霸的图，式样是同一款。(怎么贴补上？）
给个连接吧，大家只看图片就可以了。
http://compare.ebay.com/like/320855493873?var=lv |
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G******e
发帖数: 9567 | 33 LD 昨天下班回来，说公司开会，一个人做报告介绍新的策略。讲到公众对这个新策略
的反应时，打出幻灯片的题目是：Pubic Reaction to the New Strategy
（你,明白？） |
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k*****j
发帖数: 205 | 34 //??
what kind of reaction? |
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X******n
发帖数: 914 | 35 最近做LR reaction，假阳性非常高，不知道是怎么回事？就是不加entry clone的反应
，也会有很多colony长出来。 |
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f*****n
发帖数: 970 | 36 fogler reaction engineering 25刀+运费
Fluid Mechanics 20刀+运费
quickpay支付 |
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s*******h
发帖数: 3731 | 37 日啊。。有样东西叫文献。。。
实在太懒，又有钱的话，我找小弟给你合成好了。。。
reaction. |
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p****u
发帖数: 239 | 38 该打，我打字的时候就感觉差个谁来着
Tsuji-Trost reaction 是一个模板反应对于设计合成手性配体的来说 |
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z*h
发帖数: 773 | 41 Can we generate and vote the most important chemical reactions in humankind
and modern society?
The first two are:
(1) photosynthesis: CO2 + H20 + hv --> CH2O (carbohydrate) + O2
(2) respiration: CH2O + O2 --> CO2 + H2O + (ATP)
Burning
(3) Coal Combustion: C + O2 --> CO2
(4) Hydrocarbon combustion: CnHm + O2 --> CO2 + H2O
For chemical industries
C + 2H2O --> CO2 + 2H2 or (using methane)
N2 + 3 H2 --> 2 NH3
Making sulfuric acid
Making Cl2 by electrolysis
Oil cracking and reforming |
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S*****n
发帖数: 6055 | 42 I'd vote for refinery reactions
humankind |
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a*******g
发帖数: 3500 | 43 Do not forget the chemical reactions in iron and steel industries:
Fe2O3+C=Fe+CO
Fe2O3+CO=Fe+CO2 |
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l****e
发帖数: 157 | 45 5-Hydroxyindoleacetic acid (5-HIAA)和甲醛、蛋白质发生Mannich reaction时，是
在吲哚的哪个环和位点反应？是在酚羟基边上的两个碳上吗？蛋白质的反应位点是不是
在赖氨酸的氨基上？谢谢 |
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y***o
发帖数: 416 | 46 A Polymerization Kinetics Comparison between a Metallocene Catalyst
Activated by Tetrakis(pentafluorophenyl) Borate and MAO for the
Polymerization of Ethylene in a Semi-batch Solution Reactor
Macromolecular Reaction Engineering
Volume 5, Issue 9-10, pages 418–430
http://onlinelibrary.wiley.com/doi/10.1002/mren.201100015/abstr
多谢了！ |
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z*******g
发帖数: 54 | 47 两位匈牙利裔作者的那本strategic applications of named organic reactions,被
corey等评价极高。里面很多反应，在教材里都没有提人名。 怎么在书里都冒出来成了
人名反应 ？ 包括很多老的杂环合成，甚至普通的羰基α-烷基化也成了人名反应。 |
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y***e
发帖数: 6082 | 48 I tried ......COCl + H2N-CH2-CH2-......at 60度，in CHCl3 using EDC to absorbin
g HCl produced in the reaction
polymer-COCl + HO-CH2-CH=CH2?
at room temperature. |
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c*s
发帖数: 2145 | 49 HCl and = no reaction ba? |
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w********h
发帖数: 12367 | 50 there should be some small fraction of saturation.
so, need to get rid of HCl as soon as possible to compete with this reaction. |
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