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全部话题 - 话题: real
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c*****t
发帖数: 520
1
随着Real Madrid 和Barcelona 的欧冠半决赛次回合以1:1 收场,Mourinho在Madrid
执教的首赛季成绩也基本确定。虽然Real Madrid在 国王杯决赛获胜,不至于出现连续
3 赛季无冠的尴尬局面,但在欧冠半决赛被Barcelona 直接淘汰,联赛若不连出惊天冷
门也将位居Barcelona之后。
以我个人的观感,Real Madrid 在面对Barcelona 时,很难在中路组织有效进攻,是本
赛季无法取得理想成绩的最大原因。
在联赛客场的0:5 惨败后,Real Madrid 在连续3 场对Barcelona 的比赛中,均摆出3
名防守型中场,采取侵略性很强的防守反击战术。直到欧冠半决赛次回合,阵容不整且
必须争胜的Real Madrid 终于变阵,我们也可以由此剖析Real Madrid 面对Barcelona
的进攻战术。
为了扳回欧冠半决赛首回合的0:2 ,Real Madrid 在客场首发4 名进攻球员。Higuain
突前,Kaka居中,C. Ronaldo和Di Maria在上半场前段作为逆足边锋出场。结果却是进
攻毫无章法。C. Ron... 阅读全帖
J*K
发帖数: 331
2
【 以下文字转载自 Chinook 俱乐部 】
发信人: JFK (John F. Kennedy), 信区: Chinook
标 题: Is the Real-Estate Rebound for Real? zz
发信站: BBS 未名空间站 (Sun Aug 5 16:39:09 2012, 美东)
For investors, "home" is no longer a four-letter word.
The real-estate sector, for the first time in years, is serving as a beacon
of relative strength in an otherwise weak economy. Standard & Poor's on
Tuesday reported that home prices in its S&P/Case-Shiller 20-city index rose
0.9% in May from the prior month, after adjusting for seasonal t... 阅读全帖
j*******7
发帖数: 6300
3
来自主题: TrustInJesus版 - [ZT] What is Real?
http://www-scf.usc.edu/~kallos/what_is_real.htm
What is real?
Take for example the first successful theory of gravity, Ptolemy's system of
epicycles. It was used before Newton to describe the motion of the planets,
by assuming that the earth was static in the center of the world and the
planets and the sun revolved around it. It gave relatively accurate results
for astronomical observations. Was it real? I guess not. It just helped the
calculations, correctly, to be carried out.
Then Newton's th... 阅读全帖
c*****t
发帖数: 520
4
来自主题: Soccer版 - Real Madrid次回合还能打
我对Real Madrid 的欧冠半决赛次回合战术,做了些个人预计,请大家指教。
首先我们看Real Madrid 现阶段的战术。C. Ronaldo无疑是Real Madrid 的进攻核心。
另外迄今为止尚未起到决定性作用的Adebayor,其实是很能丰富战术的。
不少人或许认为Mourinho过于放纵C. Ronaldo单打独斗,但我并不认为这是什么问题。
事实上C. Ronaldo从来就不是顶级的进攻组织者。他在Manchester United 时期,对阵
强队时最见效的进攻方式,也还是建立在强悍的身体素质上的突破,远射,和头球。近
期对阵Barcelona 的3 场比赛,他确实在有些可以传球的场合,选择了自己射门。但是
他的不少射门机会,也是靠强健体魄硬拼出来的,其他人在相同条件下未必能形成有威
胁射门。对阵Barcelona ,快速反击的机会要远多于阵地进攻,给C. Ronaldo充分自由
发挥的空间,期待他在乱战中改变比赛结果,也是合乎情理的选择。
Real Madrid 在最近3 场对阵Barcelona 的比赛中,把Pepe的位置提前到中场,不但增
加了防守强度,对进... 阅读全帖
k*********l
发帖数: 1218
5
同意,我自己是women,这个口号不可能是women提出来的,这个是男性的一种思维,带
有一种非常天生的对women的discrimination
认为好多women其实不是women,real women口号会得罪好多women
我建议要们就不要打这个标语,打其他的都行
发信人: katharinezl (linlin), 信区: USANews
标 题: Re: 提出real women口号的是women吗?
发信站: BBS 未名空间站 (Sat Oct 29 16:21:09 2016, 美东)
不得不再坚持一下,如果告诉我提出real women口号的是women,并且组织者里大多数
women都觉的没有问题,我就不说了
这些口号应该要让代表的人提提意见,women for trump的口号就应该让women来决定。
chinese american,latino,african etc 都应该自己决定口号怎么弄。
women就是women,real一加,先不少这个词的定义不清不楚,个人理解都会不同,这个
口号一出,非常offensive。
大家会不会在men 前面加定义,... 阅读全帖
k*********l
发帖数: 1218
6
请转达mitbbs上一些women对这个real women口号的担忧
发信人: katharinezl (linlin), 信区: USANews
标 题: Re: 提出real women口号的是women吗?
发信站: BBS 未名空间站 (Sat Oct 29 16:21:09 2016, 美东)
不得不再坚持一下,如果告诉我提出real women口号的是women,并且组织者里大多数
women都觉的没有问题,我就不说了
这些口号应该要让代表的人提提意见,women for trump的口号就应该让women来决定。
chinese american,latino,african etc 都应该自己决定口号怎么弄。
women就是women,real一加,先不少这个词的定义不清不楚,个人理解都会不同,这个
口号一出,非常offensive。
大家会不会在men 前面加定义,white?true?real?brave? 不会!
会不会在black men前面加real?hard-working?不会!
除非在美国大选历史上对real women有过精准的定义,否则造成的反感... 阅读全帖
k*********l
发帖数: 1218
7
来自主题: USANews版 - 为什么不能用REAL???
带有歧视色彩,见我的帖子
发信人: katharinezl (linlin), 信区: USANews
标 题: Re: 提出real women口号的是women吗?
发信站: BBS 未名空间站 (Sat Oct 29 16:21:09 2016, 美东)
不得不再坚持一下,如果告诉我提出real women口号的是women,并且组织者里大多数
women都觉的没有问题,我就不说了
这些口号应该要让代表的人提提意见,women for trump的口号就应该让women来决定。
chinese american,latino,african etc 都应该自己决定口号怎么弄。
women就是women,real一加,先不少这个词的定义不清不楚,个人理解都会不同,这个
口号一出,非常offensive。
大家会不会在men 前面加定义,white?true?real?brave? 不会!
会不会在black men前面加real?hard-working?不会!
除非在美国大选历史上对real women有过精准的定义,否则造成的反感和对lgbt的
discrimination
... 阅读全帖
k*********l
发帖数: 1218
8
不得不再坚持一下,如果告诉我提出real women口号的是women,并且组织者里大多数
women都觉的没有问题,我就不说了
这些口号应该要让代表的人提提意见,women for trump的口号就应该让women来决定。
chinese american,latino,african etc 都应该自己决定口号怎么弄。
women就是women,real一加,先不少这个词的定义不清不楚,个人理解都会不同,这个
口号一出,非常offensive。
大家会不会在men 前面加定义,white?true?real?brave? 不会!
会不会在black men前面加real?hard-working?不会!
除非在美国大选历史上对real women有过精准的定义,否则造成的反感和对lgbt的
discrimination
老床已经是racist的label了,这个标语一出更加做实discrimination 对lgbt
women就是women,没有real和不real的区别,即使是个生理上是男的,如果自己label
是women,我也认为他也是real women
我想问组织者放... 阅读全帖
m****p
发帖数: 404
9
原文
http://forums.studentdoctor.net/showthread.php?t=884279
念药学院的学长学姐有什么评论么
德州 加州 pharmacy school 一家一家的开 2015-2020 铁定会过饱和了吧
最后几段是讲his family 自己开店30年的经验/挫折
还有文中提到的 MTM 是什么缩写?
1st -- Before you jump on me, understand this: I am only speaking about what
I know from extensive analysis of the California market (maybe some of this
will translate into other states but again this is for California people
primarily). If you attend, UCSF, USC, Western, UCSD, etc this job market
post is for you.
2nd -- Feel... 阅读全帖
m****s
发帖数: 494
10
What is most important for America and the next president? For me, the
answer is obvious — we have lacked leadership in both parties, and because
of this leadership deficit, the American people live in a nation less safe
and in a world that is more uncertain. Americans deserve real leadership in
the White House, and the choices Americans have in this presidential
campaign are clear.
What passes for leadership in Washington is really more about position,
seniority and the influences of special in... 阅读全帖
c*********g
发帖数: 73
11
来自主题: Living版 - Antitrust law and real estate
Sherman Antitrust Act is the law which is intended to encourage the freedom
in pricing so that a few big players would not collude to take advantage of
the customers. For example, if Microsoft, Google and Apple reach an
agreement to all raise their mobile platform price by 200% and keep the
prices fixed for the next 5 years, the consumer would not have much choice
but to pay the higher prices because there is really not much other choices
other than those three providers in terms of mobile platf... 阅读全帖
u*e
发帖数: 965
12
http://finance.yahoo.com/education/real_estate/article/101456/1
10 Things Your Real Estate Broker Will Not Tell You
----------------------------------------------------------------------------
----
.1. "Your open house is really a party for me."
Hire a real estate broker to sell your home and one of the first things he'
ll likely suggest is hosting an open house, so potential buyers can casually
check out your property on a weekend afternoon. While open houses are
promoted as a great way of find... 阅读全帖
c**i
发帖数: 6973
13
来自主题: ChinaNews版 - China Real Time
(1) Carlos Tejada, Government Puts Focus on Illicit Tollbooths. China Real
Time, Oct 7, 2011
http://blogs.wsj.com/chinarealtime/2011/10/07
/government-puts-focus-on-illicit-tollbooths/
("Illegal tollbooths often begin as authorized toll-collecting stations that
local officials keep open well past the point at which they have met their
highway-funding purposes. Chinese regulations allow tolls to be collected
for a maximum of 15 years after a highway opens. A 2005 audit showed more
than 4,300 tol... 阅读全帖
o**y
发帖数: 1466
14
来自主题: USANews版 - real women 没有任何问题
这个口号没有问题,把人分成很多群,其中有一个群就是real women, by whatever。
有的群支持川普,有的群不支持川普,而这个real women恰好支持川普,所以口号飞上
天了,就这么简单,谁也不冒犯,如果你认为你是real woman,而且也正好喜欢川普,
很好。如果你认为自己是real woman但不支持川普,没问题,标语没说all real women
.如果你不认为自己是real woman,这里没你的事,一边乘凉去。
唯一可能被冒犯的是川普,川普想,fake women我也喜欢嘛,她们的标语在哪里?
不過话说回来,Women for Trump其实就足够好了。

发帖数: 1
15
来自主题: USANews版 - 加不加real各有何利弊?
不加Real就是Women for Trump。非常好的message,反击主流媒体攻击川普是性别歧视
者。
不加Real没有坏处。
加Real就是说有些women不是real,必然得罪一部分人和他们的同情者。也许他们中的
部分人本来打算谁也不投,加Real会刺激他们出来投老妖婆。
加Real会给主流媒体机会“证明”川普支持者是恐同和性别歧视者。
绝对是被坑了。
s*********l
发帖数: 213
16
update: 鉴于huaren和买买提上很多反对REAL的帖子,决定把REAL这个字拿掉了,周一
飞的是Women For Trump。
转达组织者的message:
请放心,组织者里面是有黑人和白人的广告专家,大家经过反复磋商才确定了这几个标
题。华人组织者想用African,但黑人说他们其实不喜欢African American的说法,还
有南美黑人跟非洲都不沾边,真正的黑人说叫Black让他们更自豪。更能代表他们发出
的吼声。
real woman也是白人组织者建议的,争论了好几天才确定。华人组织者说用women,美
国人说real woman在美国人的理解是内心强大的,强壮的,正义的意思,很多LGBT也喜
欢自称real woman,不会得罪她们的
问过了:这次费城5排飞是和黑人组织一起做的。他们希望这样写的。
brandnewvest 发表于 10/28/2016 8:56:30 PM
不是全部华人捐款的。这次和好几个organization together的。
其中有个组织就叫“real woman for trump ".
brandnewvest 发表于 10/28... 阅读全帖
B********e
发帖数: 1062
17
非常同意看法
[在 katharinezl (linlin) 的大作中提到:]
:请转达mitbbs上一些women对这个real women口号的担忧
:不得不再坚持一下,如果告诉我提出real women口号的是women,并且组织者里大多数
:women都觉的没有问题,我就不说了
:这些口号应该要让代表的人提提意见,women for trump的口号就应该让women来决定
。chinese american,latino,african etc 都应该自己决定口号怎么弄。
:women就是women,real一加,先不少这个词的定义不清不楚,个人理解都会不同,这
个口号一出,非常offensive。
:大家会不会在men 前面加定义,white?true?real?brave? 不会!
:会不会在black men前面加real?hard-working?不会!
:除非在美国大选历史上对real women有过精准的定义,否则造成的反感和对lgbt的
:discrimination
:老床已经是racist的label了,这个标语一出更加做实discrimination 对... 阅读全帖
p******n
发帖数: 2449
18
来自主题: USANews版 - LOCK the real Woman hillary Clinton up
Real woman hillary Clinton for jail
Real woman hillary Clinton for jail
Real woman hillary Clinton for jail
Real woman hillary Clinton for jail
Real woman hillary Clinton for jail
Real woman hillary Clinton for jail
Whatever we want hillary for jail
Hillary for jail for jail for jail for jail
Hillary deserve it and hillary is irredeemable!
r******y
发帖数: 3838
19
In case there was any doubt about the increased horsepower found in the iPad
2, our sister site Touch Arcade
has posted a video demonstrating a forthcoming update to Firemint's Real
Racing 2 HD that will bring support
for full 1080p video out while also driving a separate display on the iPad
itself.
Using the video out feature of an iPad 2, Real Racing 2 HD will be able to
be displayed on a big screen in full
1080p resolution while the device's 10-inch touchscreen shows a track map
and race stat... 阅读全帖
s********n
发帖数: 4346
20
【 以下文字转载自 WaterWorld 讨论区 】
发信人: spinozafun (花脚猫), 信区: WaterWorld
标 题: The real reasons why people are against gay marriage
发信站: BBS 未名空间站 (Sat Jun 9 21:43:39 2012, 美东)
The real reasons people oppose gay marriage
So far, we've examined the reasons everyone give for opposing gay marriage.
Let's examine now the real reasons people oppose it, even fear it:
1. Just not comfortable with the idea. The fact the people aren't
comfortable with the idea stems primarily from the fact that for many years,
... 阅读全帖
K********y
发帖数: 5068
21
谢谢介绍背景, 把你的帖子放到这里
~~~~~~~
发信人: oldgordon (高山下的花环), 信区: USANews
发信站: BBS 未名空间站 (Sat Oct 29 15:53:41 2016, 美东)
有的ID在骂争论就是搅屎棍,那你现在在干吗?
凭什么白人黑人的意见你就尽力维护,对华人的意见就要骂?
看看下面,出钱的是华人组织者!
"real woman也是白人组织者建议的,争论了好几天才确定。华人组织者说用women,美
国人说real woman在美国人的理解是内心强大的,强壮的,正义的意思,很多LGBT也喜
欢自称real woman,不会得罪她们的。其中一个组织的名字就叫做:“real womanfor
trump ".
2, 黑人组织要发动捐款的,但最后华人组织者自己出钱是由于时间不够了,等不起
3,有争议的两条口号是黑人组织提出来的,并且不肯让步改变
4,华人组织者就real women一词与黑人组织者进行了一整天的争议,甚至考虑取消这
次飞行,最后自己被说服了"
K********y
发帖数: 5068
22
谢谢介绍背景, 把你的帖子放到这里
发信人: oldgordon (高山下的花环), 信区: USANews
发信站: BBS 未名空间站 (Sat Oct 29 15:53:41 2016, 美东)
有的ID在骂争论就是搅屎棍,那你现在在干吗?
凭什么白人黑人的意见你就尽力维护,对华人的意见就要骂?
看看下面,出钱的是华人组织者!
"real woman也是白人组织者建议的,争论了好几天才确定。华人组织者说用women,美
国人说real woman在美国人的理解是内心强大的,强壮的,正义的意思,很多LGBT也喜
欢自称real woman,不会得罪她们的。其中一个组织的名字就叫做:“real womanfor
trump ".
2, 黑人组织要发动捐款的,但最后华人组织者自己出钱是由于时间不够了,等不起
3,有争议的两条口号是黑人组织提出来的,并且不肯让步改变
4,华人组织者就real women一词与黑人组织者进行了一整天的争议,甚至考虑取消这
次飞行,最后自己被说服了"
K********y
发帖数: 5068
23
来自主题: USANews版 - Real women让人一看就很反感
谢谢介绍背景, 把你的帖子放到这里
发信人: oldgordon (高山下的花环), 信区: USANews
发信站: BBS 未名空间站 (Sat Oct 29 15:53:41 2016, 美东)
楼主在骂白人组织者,黑人组织者还是华人组织者?争论就是搅屎棍,那你现在在干吗?
凭什么白人黑人的意见你就尽力维护,对华人的意见就要骂?
看看下面,出钱的是华人组织者!
"real woman也是白人组织者建议的,争论了好几天才确定。华人组织者说用women,美
国人说real woman在美国人的理解是内心强大的,强壮的,正义的意思,很多LGBT也喜
欢自称real woman,不会得罪她们的。其中一个组织的名字就叫做:“real womanfor
trump ".
2, 黑人组织要发动捐款的,但最后华人组织者自己出钱是由于时间不够了,等不起
3,有争议的两条口号是黑人组织提出来的,并且不肯让步改变
4,华人组织者就real women一词与黑人组织者进行了一整天的争议,甚至考虑取消这
次飞行,最后自己被说服了"
K********y
发帖数: 5068
24
谢谢介绍背景, 把你的帖子放到这里
发信人: oldgordon (高山下的花环), 信区: USANews
发信站: BBS 未名空间站 (Sat Oct 29 15:53:41 2016, 美东)
楼主在骂白人组织者,黑人组织者还是华人组织者?争论就是搅屎棍,那你现在在干吗?
凭什么白人黑人的意见你就尽力维护,对华人的意见就要骂?
看看下面,出钱的是华人组织者!
"real woman也是白人组织者建议的,争论了好几天才确定。华人组织者说用women,美
国人说real woman在美国人的理解是内心强大的,强壮的,正义的意思,很多LGBT也喜
欢自称real woman,不会得罪她们的。其中一个组织的名字就叫做:“real womanfor
trump ".
2, 黑人组织要发动捐款的,但最后华人组织者自己出钱是由于时间不够了,等不起
3,有争议的两条口号是黑人组织提出来的,并且不肯让步改变
4,华人组织者就real women一词与黑人组织者进行了一整天的争议,甚至考虑取消这
次飞行,最后自己被说服了"
K********y
发帖数: 5068
25
谢谢介绍背景, 把你的帖子放到这里
~~~~~~~~~~~~~~~~~~~~~~~~~~~
发信人: oldgordon (高山下的花环), 信区: USANews
发信站: BBS 未名空间站 (Sat Oct 29 15:53:41 2016, 美东)
有的ID在骂争论就是搅屎棍,那你现在在干吗?
凭什么白人黑人的意见你就尽力维护,对华人的意见就要骂?
看看下面,出钱的是华人组织者!
"real woman也是白人组织者建议的,争论了好几天才确定。华人组织者说用women,美
国人说real woman在美国人的理解是内心强大的,强壮的,正义的意思,很多LGBT也喜
欢自称real woman,不会得罪她们的。其中一个组织的名字就叫做:“real womanfor
trump ".
2, 黑人组织要发动捐款的,但最后华人组织者自己出钱是由于时间不够了,等不起
3,有争议的两条口号是黑人组织提出来的,并且不肯让步改变
4,华人组织者就real women一词与黑人组织者进行了一整天的争议,甚至考虑取消这
次飞行,最后自己被说服了"
K********y
发帖数: 5068
26
谢谢介绍背景, 把你的帖子放到这里
发信人: oldgordon (高山下的花环), 信区: USANews
发信站: BBS 未名空间站 (Sat Oct 29 15:53:41 2016, 美东)
楼主在骂白人组织者,黑人组织者还是华人组织者?争论就是搅屎棍,那你现在在干吗?
凭什么白人黑人的意见你就尽力维护,对华人的意见就要骂?
看看下面,出钱的是华人组织者!
"real woman也是白人组织者建议的,争论了好几天才确定。华人组织者说用women,美
国人说real woman在美国人的理解是内心强大的,强壮的,正义的意思,很多LGBT也喜
欢自称real woman,不会得罪她们的。其中一个组织的名字就叫做:“real womanfor
trump ".
2, 黑人组织要发动捐款的,但最后华人组织者自己出钱是由于时间不够了,等不起
3,有争议的两条口号是黑人组织提出来的,并且不肯让步改变
4,华人组织者就real women一词与黑人组织者进行了一整天的争议,甚至考虑取消这
次飞行,最后自己被说服了"
c**********g
发帖数: 824
27
这个组织只代表了自己。大家说的是为什么不代表更广泛意义的woman?为什么要限定
到只有“real woman”支持Trump。
你让他们自己定义一下,啥叫real-woman。
网上搜到的一段:
A phrase normally used by fat middle-aged female journalists to make their
fat middle-aged readers feel better about them selves. They use to somehow
imply that any woman that isn’t overweight isn’t a “real woman” Also
used by the disgusting dove campaign for “real beauty” which uses this
bullshit to sell soap. This word is synonymous with the word “curvy” which
has also lost its real meaning.
B********e
发帖数: 1062
28
有魄力, 顶
[在 sistersnail (共用马甲) 的大作中提到:]
:update: 鉴于huaren和买买提上很多反对REAL的帖子,决定把REAL这个字拿掉了,周
一飞的是Women For Trump。
:转达组织者的message:
:请放心,组织者里面是有黑人和白人的广告专家,大家经过反复磋商才确定了这几个
标题。华人组织者想用African,但黑人说他们其实不喜欢African American的说法,还
:有南美黑人跟非洲都不沾边,真正的黑人说叫Black让他们更自豪。更能代表他们发出
:的吼声。
:real woman也是白人组织者建议的,争论了好几天才确定。华人组织者说用women,美
:国人说real woman在美国人的理解是内心强大的,强壮的,正义的意思,很多LGBT也
喜欢自称real woman,不会得罪她们的
:问过了:这次费城5排飞是和黑人组织一起做的。他们希望这样写的。
:brandnewvest 发表于 10/28/2016 8:56:30 PM
:不是全部华人捐款的。这次和好几个organization together的。
:........ 阅读全帖
J*******Z
发帖数: 528
29
新年快乐!
My name is Jennifer Zheng, I'm an experienced real estate agent. See my
personal testimony below. 我希望用我的经验和我的dedication帮你找到你的理想家
.可以打电话或email给我这样我们有个大致了解,然后我可以set up一个大约30分钟会
面to get started. If you need reference from me, I'd be more than happy to
give my most recent clients reference to you.
Looking forward to hearing from you.
Jennifer
Buying and selling homes is different from pure merchandize exchange. It’s
a life event for you! Unfortunately, not every real estate agent realizes it
o... 阅读全帖
k*****a
发帖数: 1518
30
Real estate firm apologizes after employees pose as buyers in news stories
Andrea Woo
Vancouver — The Globe and Mail
Published Thursday, Feb. 14 2013, 4:22 PM EST
Last updated Thursday, Feb. 14 2013, 11:15 PM EST
A Vancouver real estate marketing company is apologizing for having two
employees pose as prospective home buyers in televised newscasts on a
supposed spike in sales around the Lunar New Year.
The two young women – presented as house-hunting sisters whose parents
would be in town from C... 阅读全帖
s********n
发帖数: 4346
31
The real reasons people oppose gay marriage
So far, we've examined the reasons everyone give for opposing gay marriage.
Let's examine now the real reasons people oppose it, even fear it:
1. Just not comfortable with the idea. The fact the people aren't
comfortable with the idea stems primarily from the fact that for many years,
society has promoted the idea that a marriage between members of the same
sex is ludicrous, mainly because of the objections raised above. But if
those objections don't m... 阅读全帖
w*b
发帖数: 3001
32
来自主题: Love版 - a real man reads
If you don't agree, go read the column of some guy from Arkansas who thinks
owning a pickup truck is what defines a man. You'll see how far you get with
his advice.
Trait #1: A real man is strong
A real man doesn't cry, doesn't moan, doesn't complain, doesn't get sick,
and doesn't need to go to the doctor every time he sneezes. A real man makes
decisions and lives with the consequences. A real man accepts
responsibility for his actions and his words. A real man is firm. If life is
a b*tch, a rea
y*******a
发帖数: 303
33
我是在一种genomic information未知的植物里做real-time PCR。此植物为四倍体,且
自交不亲和。一般是先根据同源性设计引物扩部分DNA片段, 然后测序再设计real-
time PCR的引物。拿到引物我会先将Wildtype plant的cDNA用普通PCR仪扩增试一下退
火温度并且跑胶检验40个循环后是否条带单一。确定是单一条带了,会进行大量real-
time实验(Biorad-sybr)。这时悲剧就发生了。有如下一些问题:
1. 同一种cDNA sample的3个技术重复,melting curve不同,有时全是单峰,但不重合
,相差很远;有时3个重复里有单峰也有多峰。
2. 不同cDNA sample之间(WT vs. TG 或者stress treatment at different time
points)melting curve不同,在某个sample里很好全是单峰,在另一sample里就乱了。
3. real-time后跑胶,的确很多带。可是之前用普通PCR扩增,相同program,跑胶是一
条带。
自己的分析是:因为是四倍体,又是自交不亲... 阅读全帖
c**i
发帖数: 6973
34
来自主题: ChinaNews版 - China's 2010 Real GDP Growth Rate
(1)
(a) On Jan 20, China released its real growth rate:
(i) 4Q10 up 9.8%, and
(ii) the entire year up 10.3%.
(b) On Jan 31, 2011 Taiwan released its set of real growth rate
(i) 4Q10 up 6.48%, and
(ii) the entire 2010 up 10.47%.
My comment:
(a) "The word "real" means subtracting inflation from "nominal."
(b) 4Q10 data ended the streak of Taiwan's quarterly GDP growth rate over
China's for the previous quarters (three or four, I can not recall). Still
for the entire 2011, Taiwan's real GDP growth ... 阅读全帖
s********d
发帖数: 364
35
http://sports.yahoo.com/blogs/olympics-fourth-place-medal/unite
这个有意思 。。
Through 11 days of the Olympics, China leads the United States in the
official IOC medal count. But in Fourth-Place Medal's "real" medal count, a
tally that ignores judged activities masquerading as sports, Team USA has a
commanding lead over its Asian counterparts.
[Related: Olympic medals' monetary value may shock you]
Our real medal count ignores Olympic disciplines like gymnastics and diving
and instead focuses on sport... 阅读全帖
c**i
发帖数: 6973
36
来自主题: Salon版 - China Real Time
Loretta Chao, Two New Videos, Two Radically Different Chinas. China Real
Time, Jan 24, 2011.
http://blogs.wsj.com/chinarealtime/2011/01/24
/two-new-videos-two-radically-different-images-of-china/
("the other is a short, intensely violent satirical animation about a little
boy who dreams of revolution against a corrupt, abusive government. * * *
In it, a boy dreams about white rabbits, representing regular Chinese
citizens, living under the tyrannical rule of tigers who do nothing as the
rabbits’... 阅读全帖
l****z
发帖数: 29846
37
By EVAN RAMSTAD
SEOUL—The Constitutional Court ruled Thursday that a law requiring South
Koreans to use their real names on Internet forums was unconstitutional,
forcing the government to change the five-year-old regulation created to
reduce anonymous criticism of politicians and celebrities.
The court said the requirement amounts to prior censorship. It also said the
law violated citizens' privacy, was technically difficult to enforce and
was ineffective at stopping online criticism.
The ruling... 阅读全帖
l****z
发帖数: 29846
38
Mark Shea answers the question emphatically:
The real deal is this: People in flyover country are being told the
terms of their surrender. This is not about “toleration” and never has
been. It’s about the attempt to force people, against their conscience, to
approve of homosexual acts and gay “marriage”.
The reality is that the vast majority of the people this law means to
protect are not interested in
RFRA
quizzing strangers about their sex lives and then punishing tthem.
Nobody... 阅读全帖
l****z
发帖数: 29846
39
House Democrat Says Obama’s Lost Touch With the Real World: “We have the
same values, but we don’t have the same lifestyle”
Clearly this is racist and all Democrats should denounce this hate speech.
A House Democrat on Wednesday accused President Obama of losing touch
with the real world as his administration pushes for fast-track authority to
finish a sweeping trade pact.
Rep. Brad Sherman (D-Calif.), who opposes the pending Trans Pacific
Partnership and legislation to help finalize it,... 阅读全帖
k*********l
发帖数: 1218
40
首先是谁提出来要放real的,如果不同意一定要放的,请进这个帖子讨论一下,现在到
底谁是组织者不是很清楚
Women就是women,real放在那里是非常令人反感的,华人不要做这种替人数钱,又被人
卖的傻事!
do the right thing
Women for trump 口号又响亮,又简单,有united,real和不real的都united for
trump,that's it
s*********l
发帖数: 213
41
不谢 365天内所有主题
序号 标记 作者 日期 标题
1 * Stevenson Oct 29 跟川粉们推荐文学城,它
2 * Stevenson Oct 29 Real Women for Trump 还不够特别,要加上
3 * Stevenson Oct 29 打个大口号全城人都看见,一下就知道中国
人策划的。这么重
4 * Stevenson Oct 29 你们别揪结real women啦!再吵就改成
5 * Stevenson Oct 29 Chinese American也不对!2个理由!
6 * Stevenson Oct 29 都别吵了,加上一句不就行了!
7 * Stevenson Oct 29 尼马今早我最早提出real women不对的,看
看现在听谁的
8 * Stevenson Oct... 阅读全帖
S*******i
发帖数: 2018
42
来自主题: USANews版 - The Real Reason They Hate Trump
作者居然是耶鲁的computer science professor
http://www.wsj.com/articles/the-real-reason-they-hate-trump-1540148467
The Real Reason They Hate Trump
He’s the average American in exaggerated form—blunt, simple, willing to
fight, mistrustful of intellectuals.
Every big U.S. election is interesting, but the coming midterms are
fascinating for a reason most commentators forget to mention: The Democrats
have no issues. The economy is booming and America’s international position
is strong. In foreign affairs, th... 阅读全帖
r******2
发帖数: 67
43
A newly established real estate company is recuiting a sales manager. We are looking for one amazing, self-motivated, intelligent, and energetic individual to join our producing team of real estate agents.
Requirement: Bachelor degree required and master prefer.New York State real estate license holder, bilingual is must. Experience in real estate sales, transaction, and management.Education background strongly recommend.
We provide with good salary and bonus, friendly work position, and
conven
r******2
发帖数: 67
44
A newly established real estate company is recuiting a sales manager. We are looking for one amazing, self-motivated, intelligent, and energetic individual to join our producing team of real estate agents.
Requirement: New York State real estate license holder, bilingual is must. Bachelor degree required and master prefer. Experience in real estate sales, transaction, and management.Education background strongly recommend.
We provide with good salary and bonus, friendly work position, and
conven
m******z
发帖数: 608
45
不知大家怎么看。如果想投资房的话,是自己付掉25%首付的townhome,然后找房客,维
修,折旧。以后卖的时候还要付agent fee。还是用这个首付直接去买real estate
mutual fund。For example,去年TIAA Real Estate的Annual return 也有13%。看tiaa
的那个投资组合说:“The TIAA Real Estate Account seeks favorable long-term
returns primarily through rental income and appreciation of real estate
investments owned by the account.” 而且随时可以兑现。
m*****i
发帖数: 4342
46
来自主题: Stock版 - 说说我的三个Real Estate Holding
Currently, I mainly hold and swing trade three real estate holding:
GKK, RAS and NCT.
1) I like GKK just for one simply reason, strong cash flow each quarter.
I estimated the cash flow was around 20 million or 0.40/per share quarter.
Or 1.60/per year.
Biggest problem: They have around 700 million loans need to roll over in
March/20111. If they can successful roll over the loan, the stock should
trade much higher(over $5), otherwise, the stock could crashed down to as
low as 1.50 in my estimate.
... 阅读全帖
m******1
发帖数: 86
47
来自主题: Stock版 - Weibo real-name verification
Folks:
I just registered a weibo account to see how the real-name verification
works. here is what I found out:
1. Real name verification only apply to accounts with massive followers,
which should already being verified before the rules are published.
2. no real name verification for regular accounts which I think make sense
as who cares those accounts that had less than 100 followers.
If my finding is correct, I believe the article in below links make sense.
let me know what you think. thanks.... 阅读全帖
j******1
发帖数: 212
48
请教报税达人一个问题,请问如下情况是不是可以用 materially participating real
estate professional 报税
1. 2016 有agent 执照
2. 2016 4个月full time job,但不是跟房地产相关
3. 家庭收入>15W
4. 投资房自己管理, 符合每个房750hr 的条件。
5. 一个投资房的损失是 1.9w
我的状况是,虽然有agent 执照,但是并没有很积极的带人看房子,年交易额(1099)
也非常少。在没有full time 工作的时候主要打理房子。
这样的状况,2016 年是不是可以用materially participating real estate
professional 来避开15w 以上 passive losses 的cap.
如果,2017年我不当agent 了,也不full time 工作,自己打理投资房,是不是也可以
算做materially participating real estate professional。 换句话说,就是如果没
有agent 执照,其他条件不变,是不是也算rea... 阅读全帖
J*******Z
发帖数: 528
49
来自主题: SanDiego版 - Best in Class Real Estate Agent
Buying and selling homes is different from pure merchandize exchange. It’s
a life event for you! Unfortunately, not every real estate agent realizes it
or cares about it. But I do full heartedly! Because I was you years back,
having experienced the hesitation and excitement of buying my first home,
the calculating on purchasing my first investment property, the anxiety on
selling my first house! I’m a realtor with exceptional integrity and detail
oriented mind, a best in class realtor that is go... 阅读全帖
R*******z
发帖数: 44
50
来自主题: Seattle版 - REAL ESTATE BROKER执照挂靠?
如果考一个华州的REAL ESTATE BROKER执照以便给自己买房,是否需要挂靠到REAL
ESTATE MANAGING BROKER公司?如果想保持自己的REAL ESTATE BROKER执照一直有效,
是否一定要一直挂靠到REAL ESTATE MANAGING BROKER公司?
Thanks a lot!
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