G***l
发帖数: 355 | 1 去修车，被告知要reprogram PCM，他们那做不了，要去dealer那做。我上网查了下
reprogram pcm，网上有很多讲如何DIY的，说以前设备只有dealer有，后来因为什么法
案，这个必须要public，所以现在我们可以diy了。既然这样，那修车的店应该也能买
到啊，问了好几家修车店，都说不行，dealer又死贵，哎。 |
|
Z**4
发帖数: 1904 | 2 请教一下carfax熟悉的同学, 我最近查了几个车的记录, 有的车有有保养的记录, 有的
没有. 请问没有是正常的吗? 有记录的车那些记录是完整的还是有的有,有的时候没有?
另外一个车的两次保养记录都包括Computer reprogrammed, 还有GPS/Navigation
module/computer reprogrammed, 这属于正常保养范围还是说这个车ECU老有问题需要
program?
多谢! |
|
a**u
发帖数: 8107 | 3 这种CPO的车， Engine/powertrain computer/module reprogrammed，是不是有什么大
问题？
04/07/2014
Braman Honda of Palm Beach
Greenacres, FL
561-966-5000
bramanhondapb.com
Maintenance inspection completed
Washed/detailed
Air suspension checked
Air suspension compressor replaced/repaired
Engine/powertrain computer/module reprogrammed
Oil and filter changed
Front brake pads replaced and rotors resurfaced
Rear brake pads replaced and rotors resurfaced |
|
a**u
发帖数: 8107 | 4 二月份做过reprogram了，4月份又reprogram,然后被卖到现在的dealer这里
别的CPO没看到这样的report，所以觉得心里不爽。 |
|
E*****1
发帖数: 1468 | 5 这个reprogram没有想象那么容易，我focus也是因为原来的电子锁连接钥匙链部分断裂
导致和车钥匙分开，买了俩新的，结果按照操作手册上在小范围转动4下打开reprogram
程序死活也开不动 |
|
h***s
发帖数: 2499 | 6 新买的遥控器，电池也是新的。按照说明reprogram了，最后按了lock以后，门也自动c
ycle lock and unlock,表示成功了。
但是遥控器上没一个钮工作，如果receiver坏了前面reprogram应该不会成功吧？按了l
ock然后门cycle lock and unlock显然表明keyfob和receiver有交流。
清大虾指点 |
|
O******e
发帖数: 4845 | 7 Reprogrammed Stem Cells Hit a Roadblock: Reprogramming Cells Leads to Ge
nomic Aberrations
ScienceDaily (Feb. 21, 2011) It's a discordant note in the symphony of
good news that usually accompanies stem cell research announcements. St
em cells hold enormous promise in regenerative medicine, thanks to their
ability to regenerate diseased or damaged tissues. They have made it po
ssible to markedly improve the effectiveness of many medical treatments |
|
L***m
发帖数: 4594 | 8 【 以下文字转载自 gardening 讨论区 】
发信人: LAiam (老虎油), 信区: gardening
标 题: Sprinkler Timer 停电后 再 Reprogramming 失败 (转载)
发信站: BBS 未名空间站 (Thu Jun 4 02:38:57 2015, 美东)
发信人: LAiam (老虎油), 信区: Living
标 题: Sprinkler Timer 停电后 再 Reprogramming 失败
发信站: BBS 未名空间站 (Thu Jun 4 01:42:35 2015, 美东)
用手动也没有反应， 是什么原因请大拿支招。
我用的是obit easy |
|
g********i
发帖数: 50 | 9 车是2005年的C240，最近觉得车钥匙不大好使，必须要离车门很近才能打开车。后来发
现好像是RF不坏了，只能靠IF才能工作。
打电话问dealer说要$220换个新钥匙。后来看ebay有人卖用过的remote key，不知道各
位有没有人知道可不可以买used key来自己reprogram。。。？
多谢！ |
|
t**x
发帖数: 20965 | 10 lexus一把278刀
不过reprogram没有收钱 |
|
d*******3
发帖数: 6550 | 11 你这种情况有可能是电池没电了，没必要reprogram, 看网上有没有教程换电池，反正
便宜，值得一试。如果不行只有dealer $220换一把新的吧。 |
|
A*******0
发帖数: 1705 | 12 好久没来车版逛，有问题想请教大家。我的2010途观两个钥匙都没电了。换了电池后，
led灯闪，但是不能开门和锁门。handbook上面推荐的方法也不好使。dealer说可以帮
忙试一下reprogram，搞不搞得定都要65刀。如果搞不好，要再交钱诊断看时什么问题
。。。交钱办事儿好理解，可修不好还要交钱不能接受。求版里高人给支个招！多谢多
谢！ |
|
L***m
发帖数: 4594 | 13 【 以下文字转载自 Living 讨论区 】
发信人: LAiam (老虎油), 信区: Living
标 题: Sprinkler Timer 停电后 再 Reprogramming 失败
发信站: BBS 未名空间站 (Thu Jun 4 01:42:35 2015, 美东)
用手动也没有反应， 是什么原因请大拿支招。
我用的是obit easy |
|
f*******n
发帖数: 5241 | 14 A recent article by Steve edwards, a fitness and nutrition expert from
Beachbody:
5 Simple Steps to "Reprogram" Your Weight
By Steve Edwards
Your body resists change, even when it's good for you. This irritating
little survival trait has led to a passel of colloquial words that we use
when referring to how to deal with it. These include survival mode,
starvation mode, periodizational training, plateau-ing, and set point. Today
we'll tackle the latter, which is a term we use to explain why our bo |
|
L***m
发帖数: 4594 | 15 【 以下文字转载自 Living 讨论区 】
发信人: LAiam (老虎油), 信区: Living
标 题: Sprinkler Timer 停电后 再 Reprogramming 失败
发信站: BBS 未名空间站 (Thu Jun 4 01:42:35 2015, 美东)
用手动也没有反应， 是什么原因请大拿支招。
我用的是obit easy |
|
c****t
发帖数: 19049 | 16 ScienceDaily (July 9, 2011) — For the past decade, researchers have tried
to reprogram the identity of all kinds of cell types. Heart cells are one of
the most sought-after cells in regenerative medicine because researchers
anticipate that they may help to repair injured hearts by replacing lost
tissue. Now, researchers at the Perelman School of Medicine at the
University of Pennsylvania are the first to demonstrate the direct
conversion of a non-heart cell type into a heart cell by RNA transfer... 阅读全帖 |
|
h*****G
发帖数: 113 | 17 Thank you.
Lineage reprogramming is definitely very promising. As mentioned in another
post, it has been showed that fibroblast can be transformed to neuron
with only 3 TFs.
At the same time, direct programming from ES/iPS cells to somatic cells are
few. And I was not be able to find any publications. So was wondering if
someone can help me with some papers about direct programming from ES/iPS
cells to somatic cells with TFs, not stepwise extracellular signals.
Thanks.
转换 |
|
t****p
发帖数: 1504 | 18 These are good questions! I also thought about that.
Theoretically, directional differentiation is easier than reprogramming,
because the latter is not a natuarl process. There is no any reason that
transcription factors should not force cells to differentiate into specific
lineage, given the facts of iPS.
I don't think any lab has achieve this, though.
question |
|
n********k
发帖数: 2818 | 19 Historical Origins of Transdifferentiation and Reprogramming
http://www.sciencedirect.com/science/article/pii/S1934590911005
This is a nice piece from Dr. Thomas Graf, better and more comprehensive
than the piece from cell editorial in 2006 and another one from SIr John
Gurdon himself...
it is a nice and easy lunch reading for those who is outside of the field
but wants to know the history and who should be credited with the Nobel....
In my mind, there isn't much controversy here...really a very... 阅读全帖 |
|
C***2
发帖数: 62 | 20 Hello, Are there any reviewing opportunities in the field of ESC and Somatic
reprogramming? I am ready to provide CV. Thanks for your kindly help！ |
|
|
d*p
发帖数: 534 | 22 Heard of reprograming T cell to IPS, then back to T cell again., might help
a little during re-activation. don't see much change on function. |
|
m******q
发帖数: 5 | 23 博后第三年
两次独立审稿经验，帮老板审过十次左右（IF 2-15)，最近几次老板都没怎么改直接发
给editor
方向：regenerative medicine, stem cell (iPSC), cardiac and other direct
lineage reprogramming, cardiology, epigenetics, single cell genomics and
data analysis.
phD training 很全面，基本上分子，细胞，遗传，动物，以及生物信息学分析都会。
请站内。万分感谢！ |
|
b*******0
发帖数: 125 | 24 http://science.sciencemag.org/content/357/6346/102
Reprogramming my career
As I stood at the whiteboard with a marker in my hand and a five-person
interview panel of industry experts watching me, I realized I had no idea
how to perform the “simple” coding required to solve the question that was
asked. I was about to be exposed as a fraud. What had ever made me think
that I would be able to transition from medical research to data science?
That was 6 months ago. Even though that initial interview... 阅读全帖 |
|
a**i
发帖数: 7261 | 25 门经常锁不上,报错,dealer说应该需要reprogramming了,这个活必须去dealer那做了,
一般的店自己能搞定吗?dealer约的话要半个月以后了,不知道为什么这么忙 |
|
s********3
发帖数: 231 | 26 《科学》对STAP手稿的专家评审意见曝光
《科学》对传说中的STAP细胞论文的三名评审专家意见，过去一直被雪藏，今天终于
被曝光。专家对这一研究的评价不高，认为非常全面的描述性研究，如果结果确定，可
能导致发育生物学大厦的颠覆，其实是不相信这种研究结论。因为没有当时的投稿件，
所以没有办法对这些评价进行更深入分析。不过这些资料显然都在某个地方，只是拥有
的人不愿意拿出来。
Retraction Watch readers are of course familiarwith the STAP stem cell saga,
which was punctuated by tragedy last month whenone of the authors of the
two now-retracted papers in Nature committed suicide.
In June, Science‘s news section reported:
Sources in the scientific community confirm that early version... 阅读全帖 |
|
C***2
发帖数: 62 | 27 http://www.nature.com/ncb/journal/vaop/ncurrent/full/ncb2765.ht
Sequential introduction of reprogramming factors reveals a time-sensitive
requirement for individual factors and a sequential EMT–MET mechanism for
optimal reprogramming
Abstract:
Present practices for reprogramming somatic cells to induced pluripotent
stem cells involve simultaneous introduction of reprogramming factors. Here
we report that a sequential introduction protocol (Oct4–Klf4 first, then c-
Myc and finally Sox2) outperfor... 阅读全帖 |
|
O******e
发帖数: 4845 | 28 Press Release
2012-10-08
The Nobel Assembly at Karolinska Institutet has today decided to award
The Nobel Prize in Physiology or Medicine 2012
jointly to
John B. Gurdon and Shinya Yamanaka
for the discovery that mature cells can be reprogrammed
to become pluripotent
Summary
The Nobel Prize recognizes two scientists who discovered that mature, specia
lised cells can be reprogrammed to become immature cells capable of developi
ng into all tissues of the body. Their findings have revolutionised our... 阅读全帖 |
|
l**********1
发帖数: 5204 | 29 En
one aspect:
To keep stability and safety of Somatic cell Reprogramming to iPS cell
pls refer
Hamada M. et al. (2012)
Reprogramming to Pluripotency Can Conceal Somatic Cell Chromosomal
Instability.
PLoS Genet. 8: e1002913.
Abstract
ignored
These findings suggest that aneuploid cells can be selected for or against
during reprogramming depending on the W-CIN gene defect and present the
novel concept that somatic cell W-CIN can be concealed in the pluripotent
state. Thus, karyotypic analysis of s... 阅读全帖 |
|
t******k
发帖数: 553 | 30 我在英文论坛上面看到相关的帖子，有人回复说：
1）The plastic part contains the remote/遥控器 for locking/unlocking the
doors. There's also an RFID chip in there for the immobilizer/锁止器.（这个
锁止器是哪一个部位？）
2）I finally solved my problem. I ordered just a blank key shell from ebay
for only $7 including shipping. I transferred the remote and the RF chip
from my old shell and took the new key along with my second original key to
a locksmith who had the right equipment. He charged me $28 to cut the new
key.
3）the dealer refused to ... 阅读全帖 |
|
|
a****d
发帖数: 1919 | 32 You are right, iPSCs hypo is still there. It's just such a crowded field now
and people are going nuts to compete for publishing.People from out lab
just found almost identical xx cell derived iPCSs results being published.
Those disease iPSCs models will continue to water on top journals for sure.
Along with that, reprogramming becomes hotter and hotter. For the next few
years, people still have to rely on TFs for reprogramming. Large small
molecular screening is on the way to replace those TFs... 阅读全帖 |
|
h******y
发帖数: 351 | 33 second that.
这篇文章的发现固然有意思，但是能发在nature上也有赶潮流（反潮流）的因素在里面
。这篇文章的意义在于提醒人们不能忽视移植受体对iPS细胞可能存在的免疫排斥，但
是由此就把iPS一棒子打死也不免言过其实。
对于iPS细胞能否用于及时有效的器官移植，大家一直有争论。免疫排斥不论，另一个
很大的问题是及时性。毕竟很难想象在急需移植器官的时候，病人能够等待至少6个月
以上的时间，以便从体细胞开始reprogramming并生产出数亿个特定的功能细胞（器官
移植需要末端分化的细胞，否则有致癌的危险）。一个解决办法是利用胚胎干细胞，进
行所谓的预生产，这也是目前进入临床试验的两个项目（Geron的脊髓损伤和ACT的
Macular dystrophy）都利用胚胎干细胞的原因。
另一个解决办法是建立iPS Bank，在目前无法建立新的ESC系的情况下，建立尽可能多
的iPS系，理想状况是囊括所有人群。很多大的医药公司也希望建立这样的iPS bank，
原因下面详述。根据小道消息，CDI目前拿到一个建立250个iPS系的contract。这只是
开始，后面应该还有更大的。... 阅读全帖 |
|
f**u
发帖数: 346 | 34 我怎么记得在克隆羊的故事里reprogram这个词被说了很多次？难道记错了。
另外我不知道你怎么定义reprogram这个词。
我的理解，如果是从体细胞reprogram到ESC，核移植是第一个，就是当年那个克隆青蛙。
如果说是从皮肤细胞到神经元之类的reprogramming，拿到好像真是iPS出来以后的事。 |
|
s******r
发帖数: 1245 | 35 他这篇science的思路很简单，就是把yamanaka找到的那些因子全部用小分子代替，这
个东西很多实验室在做，之前已经找到了分别代替单个因子的小分子，但是合起来还是
要用到至少一个因子，差的就是临门一脚把这最后一个代替了，但大家都卡在这一步，
他们这篇文章里仔细摸出了条件，有点新东西是加了点小分子来调节甲基化。这篇文章
的意义主要是技术上的突破，就是实现了全部小分子reprogramming，证明了全小分子
这条路是可行的，这个对以后ips的应用非常有用，因为之前的所有方法多多少少都要
用到转基因，而一般认为只用小分子的话会更安全。当然他们做出来的东西到底有多大
的意义还需要更多验证，比如在其他种类的细胞特别是人的细胞上也要能重复出来，他
们文章里用的MEF本身是非常容易reprogramming的细胞，而他们做出来的效率虽然够用
了但是相当低的。
他五月份的那篇cell是一个跟其他实验室完全不同的新思路，那篇文章找到了新的因子
来reprogramming，意外的地方在于这些因子是定义胚层分化的因子，而通常人们认为
reprogramming是去分化的过程，两个相反的方向可以到达... 阅读全帖 |
|
b***2
发帖数: 348 | 36 我对本文谨慎乐观。丁胜几年前就筛出oct4加小分子的条件，这次是临门一脚把oct4也
换下了。但是另一方面，之前有报道说小鼠的oct4本来就是可取代的，而人的oct4是不
可取代的。所以在纯化学诱导人的方法出来之前，我持谨慎态度。nn【在 skycolor (
ss)的大作中提到：】n：他这篇science的思路很简单，就是把yamanaka找到的那些因
子全部用小分子代替，这n：个东西很多实验室在做，之前已经找到了分别代替单个因
子的小分子，但是合起来还是要用到至少一个因子，差的就是临门一脚把这最后一个代
替了，但大家都卡在这一步，他们这篇文章里仔细摸出了条件，有点新东西是加了点小
分子来调节甲基化。这篇文章的意义主要是技术上的突破，就是实现了全部小分子
reprogramming，证明了全小分子n：这条路是可行的，这个对以后ips的应用非常有用
，因为之前的所有方法多多少少都要n：用到转基因，而一般认为只用小分子的话会更
安全。当然他们做出来的东西到底有多大的意义还需要更多验证，比如在其他种类的细
胞特别是人的细胞上也要能重复出来，他们文章里用的MEF本身是非常容易
reprogr... 阅读全帖 |
|
z**********e
发帖数: 22064 | 37 http://www.nytimes.com/2014/03/18/world/asia/malaysia-airlines-
By MATTHEW L. WALD and MICHAEL S. SCHMIDT
WASHINGTON — The first turn to the west that diverted the missing Malaysia
Airlines plane from its planned flight path from Kuala Lumpur to Beijing was
carried out through a computer system that was most likely programmed by
someone in the plane’s cockpit who was knowledgeable about airplane systems
, according to senior American officials.
Instead of manually operating the plane’s controls,... 阅读全帖 |
|
j***k
发帖数: 2719 | 38 我的２００８ ３２８XI WAGON前一个星期电池坏了，送去BMW DEALER，被告知保修不
包括电池，换电池需要＄５８０，电池＄２３０＋REPROGRAMMING＄３５０。我当时就
晕了，问什么是REPROGRAMMING，修的人也搞不懂，反正说一定要REPROGRAM，否则电池
没有办法工作。没办法，只好付了。。。电池换好又被告知VALTSONICE MOTOR有问题需
要换掉，还好保修COVER，就换了。
上周２去拿回车，停在车库没有开。周６早上全家出门，突然车又不能发动了，只好在拖车到
DEALER。一直到今天周３DEALER才打电话来说他们还没有确定车的毛病在哪里，最早
需要下个星期才能拿车，不过应该保修是COVER的。
还好，保修是１２月到期。才３万MILE的车啊，就这样。上个星期第一次修的时候看到
一个老头在付修理费，他的２００３ ５系列坏了，修理费是７８００刀。。。晕啊。。
已经和老婆商量好，修好就去TRADE－IN，换ACURA，LEXUS， B甚至BUICK。BMW的质量
实在太烂。。。价格太贵，修不起，， |
|
z****8
发帖数: 5023 | 39 Component: ENGINE AND ENGINE COOLING
Manufacturer: NISSAN NORTH AMERICA, INC.
Potential Number of Units Affected: 653910
Year: 2005
Make: Nissan
Model: Sentra
Manufactured
from: 2004-07-13
to: 2006-07-29
Recall Date: 2007-11-14
Summary:
ON CERTAIN PASSENGER VEHICLES EQUIPPED WITH A 2.5L ENGINE, THE CRANKSHAFT
POSITION SENSOR CAN OVERHEAT CAUSING A BRIEF INTERRUPTION IN THE SIGNAL
OUTPUT FROM THE SENSOR.
IF THE INTERRUPTION IN THE SIGNAL FROM THE CRANKSHAFT POSITION SENSOR IS SO
BRIEF THAT ... 阅读全帖 |
|
o***m
发帖数: 203 | 40 09/08/2012 1 Town & Country Jeep Chrysler
Levittown, NY
516-796-5500
tcjeep.com Pre-delivery inspection completed
09/08/2012 5 Town & Country Jeep Chrysler
Levittown, NY
516-796-5500
tcjeep.com Vehicle offered for sale
01/18/2013 Chrysler Manufacturer Customer Satisfaction
Notification issued
#M38 REPROGRAM POWERTRAIN CONTROL MODULE
Click here for a copy of the owner letter for this Customer Satisfaction
Notification or call 1- 800-853-1403 i... 阅读全帖 |
|
s***c
发帖数: 1926 | 41 还是这家伙，3月22日又发的帖子说升级了软件后问题解决了。PCM SW 升级到了 83.04
版本。
Hi - bought a diesel CX5 in July 2012. I had oil changes at 2800, 4000, 7800
and an early 10k service at 8700 so I could drive it 2k without worrying.
Had a PCM reprogram at 11700klms. Now done 17370klms and the oil growth is
OK. A long 3000 Klms trip saw no measurable oil growth. I am having the oil
tested every oil change and so far it is OK.
Mazda worldwide is studiously trying to avoid the issue - even when it is
fixed by the reprogram. It s... 阅读全帖 |
|
w****2
发帖数: 936 | 42 The Proper Way to Install and Reset an ECU
June 1, 2011 5.3K 0 Is this guide helpful? 5 Report
Share:
Installing an ECU yourself is easy. This guide lists the proper way to
insall and reset an ECU.
First you have to make sure the ECU you are about to install has the same
part number as the ECU that was running the car. If the numbers match,
follow these steps:
1. Unplug the battery from the car
2. Let the car sit for fifteen (15) minutes unplugged.
3. Install the n... 阅读全帖 |
|
h***g
发帖数: 45 | 43 請問一下 在前owner賣車前的9000 mile時 在carfax上查到Computer module replaced
。請問有什麼大問題嘛？
10,000 mile service performed
Washed/detailed
20,000 mile service performed
Alternator replaced
Battery replaced
Battery/charging system checked
Engine/powertrain computer/module reprogrammed
30,000 mile service performed
Front brake pads replaced
Interior trim replaced
40,000 mile service performed
Alternator replaced Palm Springs Battery replaced
Battery/charging system checked
Computer module replaced
Computer reprogram... 阅读全帖 |
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b*****d
发帖数: 61690 | 44 The Senate CR, which will be acted on shortly, provides some reprogramming
for NIST and NASA along with additional funding for NSF and OSTP to help
these agencies better deal with the sequester. The Senate CR maintains
fiscal year 2012 appropriated levels for the Department of Energy, NIH, and
the Department of Defense. The Senate CR would still be subject to the
sequester.
On March 6, the House of Representatives passed legislation extending the
fiscal year 2013 Continuing Resolution (CR) to Se... 阅读全帖 |
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P****D
发帖数: 11146 | 45 无意中看到的，转过来大家晴天霹雳一下：
http://www.wired.com/threatlevel/2013/08/kwikset-smarkey-lock-v
Millions of Kwikset Smartkey Locks Vulnerable to Hacking, Say Researchers
By Kim Zetter
08.03.13
6:30 AM
LAS VEGAS – Locks that are used in millions of homes and residential
buildings worldwide and that are designed specifically to thwart hacking are
easily opened with both a screwdriver and wire, two researchers say.
Kwikset smartkey locks are certified Grade 1 security for residential use by
the Bui... 阅读全帖 |
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m***f
发帖数: 1622 | 46
扯淡呢，看看高最近两年发表的文章吧
CELL STEM CELL, JBC, STEM CELL, PNAS，都是很不错的
就这样还不能PROMOTION? 在美国都可以tennure了
1. Wu T., Wang H., He J., Kang L., Jiang Y., Liu J., Zhang Y., Kou
Z., Liu L., Zhang X., Gao S. (2011) Reprogramming of trophoblast stem
cells into pluripotent stem cells by Oct4. Stem Cells 2011 Feb 8.
[Epub ahead of print].
2. Kang L., Wu T., Tao Y., Yuan Y., He J., Zhang Y., Luo T., Kou Z.,
Gao S. (2010) Viable mice produced from 3-factor induced pluripotent
stem (iPS) cells through tetrapl... 阅读全帖 |
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n********k
发帖数: 2818 | 47 I'll comment from my memory, some facts could be wrong but shouldn't be too
far off....here it goes...
was just wondering how many of you are working in the related field and
knows the history...Some comments here are just so off...e.g: i would be
extremely surprised that if Thomas would have a share for iPS...the
reprogramming has been there for so long--it has a rich and very clear path,
I am sure many many have been thinking about using several factors to
reprogram to stemness...In fact there... 阅读全帖 |
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s******r
发帖数: 2876 | 48 那篇2006的cell是很突破，很大胆，但是说不上设计得很精巧吧。
I'll comment from my memory, some facts could be wrong but shouldn't be too
far off....here it goes...
was just wondering how many of you are working in the related field and
knows the history...Some comments here are just so off...e.g: i would be
extremely surprised that if Thomas would have a share for iPS...the
reprogramming has been there for so long--it has a rich and very clear path,
I am sure many many have been thinking about using several factors to
re... 阅读全帖 |
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i*****g
发帖数: 11893 | 49 因为我曾经在一个iPS group边上，所以比较关心iPS这方面。
在yamanaka之前，有这样的观念，用1-2个cDNA transformation，然后得到什么什么细胞系
这个本质是stable cell line和transgenic 路数
iPS 开辟的是reprogramming这个路线，在iPS之后，马上很多人就做了
transdifferentiation，依据同样的思路。
在Yamanaka之前，有些人搞过这个XXX细胞到iP，比如Stanford 胡继繁就有类似的想法，还申请了
专利，但毕竟没有成功。
当然，iPS有很多要细化的研究，比如gene deletion duplication trouble in the final iPS
iPS generated from somatic cell? or from few tissue stem cells in the
primary culture???
无论如何，这个是reprogramming的事情，是的，某些伙计说的，或许弄出了生物学有
史以来最大的reprogramming 泡沫，呵呵
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i*****i
发帖数: 154 | 50 老大要找的质粒应该是叫STEMCCA
Stem Cells. 2009 Mar;27(3):543-9.
Induced pluripotent stem cell generation using a single lentiviral stem cell
cassette.
Sommer CA, Stadtfeld M, Murphy GJ, Hochedlinger K, Kotton DN, Mostoslavsky G.
Abstract
Induced pluripotent stem (iPS) cells can be generated using retroviral
vectors expressing Oct4, Klf4, Sox2, and cMyc. Most prior studies have
required multiple retroviral vectors for reprogramming, resulting in high
numbers of genomic integrations in iPS cells and limiting... 阅读全帖 |
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