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发帖数: 13 | 1 I think Pierce has a very comprehensive guide for protein crosslinking
reagents. did you find anything on their website? I found almost every detail
that I needed for my exp.
however, crosslinking is a tricky thing in that it varies from protein to
protein, and the condition for crosslinking matters a lot too. if you are
doing it in the presence of crude cell lysate, i doubt if there is established
protocols to follow. you probably need to try different crosslinking reagent,
different concentrat |
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