由买买提看人间百态

boards

本页内容为未名空间相应帖子的节选和存档,一周内的贴子最多显示50字,超过一周显示500字 访问原贴
Biology版 - Re: Help! Qualify Question
相关主题
need help_how to disrupt a homodimer?蛋白质修饰 or binding or self-dimer? 怎样检测?
工业界需要哪些技术呢?用啥方法证明一个膜受体蛋白形成dimer的量或者构象发生了改变
Job Opportunity -- Protein Purification and Cell biology, SF Bay Area only我来贴一个全一点的细胞分离方法的LIST
湾区蛋白纯化的工作机会 (转载)Re: 紧急求助
什么protein assay可以区分protein和peptide?Re: GST fusion protein-Thanks, buddy, but you guys misunderstood
Re: 谁有经验? 关于dimerization 和 native gelRe: 请教:GST fusion protein
which FPLC to buy: Akta (GE) or Duo Flow (Bio-Rad)??SBIG: Channels - 5
question about protein size[合集] 请问纯化蛋白质系统
相关话题的讨论汇总
话题: question话题: qualify话题: assay话题: dimer话题: gel
进入Biology版参与讨论
1 (共1页)
s******y
发帖数: 28562
1
FPLC or HPLC Gel filtration column, viscosity assay or sedimentation velocity
assay can be used for dimer/polymer assay. Native gel is also used for such
purpose. SDS-PAGE is NOT!
Antibody? Buy it from commercial company.
Actually, for your question, you DON"T have to do those dimer things at all.
They are the properties of a protein but has NOTHING to do with purity.
A proper folding is needed, if your lab is gonna to test it's activity.
However,if only the protein sequence is corncerned (e.g.,
1 (共1页)
进入Biology版参与讨论
相关主题
[合集] 请问纯化蛋白质系统什么protein assay可以区分protein和peptide?
生物如此烧钱,大量资金外流,是中国人自己不争气!Re: 谁有经验? 关于dimerization 和 native gel
包子问:哪里能买到FPLC的200 ul或者250ul的sample loopwhich FPLC to buy: Akta (GE) or Duo Flow (Bio-Rad)??
同一个蛋白却不跑在一条带上?question about protein size
need help_how to disrupt a homodimer?蛋白质修饰 or binding or self-dimer? 怎样检测?
工业界需要哪些技术呢?用啥方法证明一个膜受体蛋白形成dimer的量或者构象发生了改变
Job Opportunity -- Protein Purification and Cell biology, SF Bay Area only我来贴一个全一点的细胞分离方法的LIST
湾区蛋白纯化的工作机会 (转载)Re: 紧急求助
相关话题的讨论汇总
话题: question话题: qualify话题: assay话题: dimer话题: gel