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Biology版 - Re: Did I get correct cDNA clone?
相关主题
怎样找到一个gene的promoter问一个关于cDNA的问题。
3X repeat 克隆请教反转录酶引起的 RNA sequence 假阳性
restriction enzyme encoded in human genome?!验证sgRNA用什么样的细胞系合适
Re: Sequence请教如何找一个基因在不同物种的cDNA,然后做sequence alignment
Can anyone make comments on RNA sequence?克隆flanking sequence有啥短平快的方法吗?
请问大家都是怎么生产抗体的?(from scratch)哪里可以找到public的next gen seq的data 或者database
问个sequencing的初级问题Sequencing 还能热多久呢?
Anybody familiar with genome assembly software?有没有谁有鼓捣生物信息服务公司的想法?
相关话题的讨论汇总
话题: cdna话题: whether话题: protein话题: did话题: correct
进入Biology版参与讨论
1 (共1页)
b********r
发帖数: 9
1

how
1.extract the total mRNA
2.use restriction enzyme to cut out the desired gene
3.design a primer and do a PCR
4.Sequence the DNA and compare with those published sequences to see whether
it encodes the kind of enzyme you want.
5.Southern Blot to see whether pure and whether it is there.
6.Express the cDNA in Ecoli and check protein activity.
7.translate the sequenced cDNA to see whether you got the correct protein.
8.Sequence the expressed protein and back translate part of the AA's that has
1 (共1页)
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相关主题
有没有谁有鼓捣生物信息服务公司的想法?Can anyone make comments on RNA sequence?
Re: 哪位大仙知道生化中K cat 的定义是什么请问大家都是怎么生产抗体的?(from scratch)
New England Biolabs Postdoc Fellowship问个sequencing的初级问题
Two postdoc positions in Redox Enzyme Engineering at the Biofuels LaboratoryAnybody familiar with genome assembly software?
怎样找到一个gene的promoter问一个关于cDNA的问题。
3X repeat 克隆请教反转录酶引起的 RNA sequence 假阳性
restriction enzyme encoded in human genome?!验证sgRNA用什么样的细胞系合适
Re: Sequence请教如何找一个基因在不同物种的cDNA,然后做sequence alignment
相关话题的讨论汇总
话题: cdna话题: whether话题: protein话题: did话题: correct