h******u
发帖数: 602 | 1 Try to treat cells with a chemical. This chemical is easy to dissolve in
DMSO. However, if adding to culture meidum, it precipiates.
How to solve this problem? Lower the concentration?
Thanks~~~~~~~~ | s******y
发帖数: 28562 | 2 Don't add the chemical to the medium,
instead, add the medium to the chemical.
For example, add the chemical to a empty 50mL tube, then quickly add 10mL
media to disperse the chemical. This should help
【在 h******u 的大作中提到】
: Try to treat cells with a chemical. This chemical is easy to dissolve in
: DMSO. However, if adding to culture meidum, it precipiates.
: How to solve this problem? Lower the concentration?
: Thanks~~~~~~~~
| r***e
发帖数: 2539 | 3 请问是什么原理？
局部浓度太大？
【在 s******y 的大作中提到】
: Don't add the chemical to the medium,
: instead, add the medium to the chemical.
: For example, add the chemical to a empty 50mL tube, then quickly add 10mL
: media to disperse the chemical. This should help
| s******y
发帖数: 28562 | 4 Yes.
【在 r***e 的大作中提到】
: 请问是什么原理？
: 局部浓度太大？
|
|
