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Biology版 - 读了那个关于中国RA的贴
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PCRLinks.com Home for PCR users一个GWAS genotype imputation的问题
这种情况一定要做Southern Blot吗各位师弟师妹,到发考题版来学习一下吧
Re: SequenceHelp: Failure of Red recombineering retrieve
总是在trouble shootingDifficult cloning - 求助
怎么GENOTYPE HOMOZYGOTE tg MICEstable clones vs. stable pool
如果genotyping结果发现一个SNP不符合HWE说明啥?有没有人用multisite gateway做克隆的?
wierd genotyping, and need help再问gateway cloning
genotype 的价格文献求助,谢谢
相关话题的讨论汇总
话题: ra话题: her话题: me话题: she话题: hr
进入Biology版参与讨论
1 (共1页)
p*******7
发帖数: 23
1
一段时间前,本想‘第一次版上发贴,真心求教’,写了下文。后真的烦不愿再提。今
天读‘中国RA的文,以及回复,希望
分享我的’drama':
长话短说,RA:发 IF 》12 的一作时我还在本科,不知’科研‘何物。我, 薄厚前活泼
开朗,心直口快(现在,真的知道是
大毛病),喜欢宏观的可以见效果的。薄厚4年了,文章去工业界多了(朋友言),留学
术界又差着。‘人生的九曲湾
中’放弃追梦. 博后到了距家最近的Lab, 虽然lab focus 与我的Ph.D. training有出入
与此RA曾为朋友,老板3 年前发现课题有潜力, assign 此 RA, 非大陆的。我还开心
,不是因有RA,而是因为是朋
友。(领域真的labor intensive, grad school 与三RA 共事,另一美国博生是我好友
,亦次次出手在大实验:3-5 小
时能拿到要的sample 就很开心乐。这后面才是set-up study.)薄厚Drama开始于这RA
的指定。几乎合作刚开始,朋
友就没了。我傻糊糊的还想继续友谊,修缮关系。直到N 年后被HR告 'forget about
friendship, you 2 will never be
friends again.' 'As a project leader, your goal is respect rather than
friendship.' (这是后话了)
俯拾即是的小事不说,landmark 如下:
第一遭:我心狂跳,终于坐下问,‘Anything that I made you unhappy?' 造化弄人
,before I could get much out
of her accusations, 老板竟空降,---RA 认为我set up to frame her.// 老板
隔日脸很长,叫我去office. 我气。
老板只问是否'hard to collaborate'. ’Yes 。。。还没说下半句,老板告知要 '
turn her to HR'. 我懵。回家告老公,听
了老公教诲 ‘都是中国人,得饶人处且饶人’。连夜email 老板,不想RA在枪口下逼
着合作,I will tryto team up with
her using interpersonal skills. (几年后一已faculty的朋友 得知,说我老板
professional, 我不professional, RA
是否一人背房贷与我屁事。不应成为我决定合作人的动机。说我公私不分。)
一年后:我心生退役,1。在周五电话中,心中感怀当年,所以电话恳谈。她亦坦诚因
何气恼。我心惊才知:
说‘Chinese but also English-speaking people’ told her I said she didn't
work hard enough。//. 这层的中国
人搬着指就这几个。抑或是RA或薄厚。 我在第一遭想一年前using interpersonal
skills to team up with her 时确有
去求教中国人--是诚心请教的;但没有对任何美国人讲过。我心凉,浪费一年解决提
高她对我的不满,其实根在
这?// 2. 一个周二中午,我终于见道她。对她讲要genotype 1 litter, 周五下午要
结果。她拍桌子,怒目而视,原话
‘Are you giving me a deadline? Are you giving me a deadline? Even xxx (the
big boss), never ever give me a
deadline.' 我狂深呼吸,问她做genotype 要多长时间。这真捅了马蜂窝,’You have
no right to ask me that
question, you don't even pay me'. 我强忍眼泪, 退了。// 3。 做人不能太自负
。至此,我仍认为我可以give it a
last try. 停了实验,面对面恳谈, 无果。Looking into her eyes, I told her "I
will not be able to work with you like
this ; it's too hard'. 她依然怒视‘ are you trying to threat me?'. 我忽然觉
得可怕:干,她说我 ’treat her as hands'
(略去无数); 不干,她说我 threat 她。// 下一天,买icecream and very
sincerely approached her, 又甭了。始发
罪状的条目不停激增,包括我让她觉得我off hour不屑于回她电话。// 4。 当晚
email 老板 sorry that I couldn't
make it to be a team. 我提出单飞。
第二遭,老板把我俩一起上交 HR. goal is to team us up。我老实的交代了问题,这
次起于genotyping。也提出我希
望知道how the other side feel what the major problem is. 我愿意有HR
professional 建议, 改进提高。后来,才
很吃惊的得知,quote HR said ' she was very sincere and very sweet, told us
wow there is no problem now
we've talked.' The suggestion I got was 'forget about friendship and aim at
respect'.// 后来头一次,也是唯
一一次,她主动找我,要跟我做。我心很累也很伤了,因为和她的关系,我都少与周围
的人交谈了。The last thing I
need would be further gossip to make the situation even harder. 坦诚说强扭的
瓜不甜,我不认为it will work.
抬头看见一滴眼泪慢慢留下。合作来,我都是被噎的,眼圈红的那一个。见她如此,我
心一下很痛。Without even
think of consequence, I told her 'don't cry please, I will work with you.' (
现在知道又错了)
第三遭,老板鲜有露面了,口头命令我是她的 ‘supervisor'。‘命令’因为告知时,
我不同易。I don't believe I could
do that since I've wittnessed even a very polished US postdoc (grad from
stanford) could not work out with
her. 那时我还感慨他傻,此RA对她会的技术真的是skillful. Anyhow 命令还是在我们
三一起的时候下了,因为老板 not
even here much, ‘you 2 have to work together. I can not afford you 2 fight.
' 结果:被命令接受“supervise“她。
第四遭,就到乐2010了。请她做1 个cloning。 把plasmid 的 一段防到 另一个
plasmid 以便做病毒。Beofre Xmas
started planning, New year 回来开始了。知道她喜独立,我就没有给detail plan。
我说希望两个月有结果。她当时旧
讲6个月的也不奇怪,有的就是难。//长话短说,3 个月,无进展。期间列了list给
老板说因为我还不停让她做别的,
如给细胞换液,perfuse 老鼠。至此,无它,只科隆。3月中,maxi-prep 3次,告我’
you have to wait since it's not
enough.' 'sorry, I will have to keep doing max-prep, ect'。The next day,
emailed me she wants a vacation. I
said 'yes, after the cloning is done'. she emailed boss saying it 'illegal'
since BY LAW she entitles xxx days
vacation per year, ect // The end, she went vacation in mid of April, with
failed attempt of cloning --- even
though for that almost 2 months she is doing NOTHING but cloning.// 1
Japanese postdoc and 1 Korean
postdoc who I've collaborated both told me 'you should get it done in 1 week
.' With that spirit, I did the
cloning from scratch in 3 weeks.// My boss told me 'being a supervisor, you
have to be sensitive.'
尾声,几周前我告知朋友我不要这个RA了,好累呀,天天象在战场上。朋友说‘是不是
发了文章你变了,而不是RA 变
了?’ 我心中酸楚呀,想想算了。我不久就走了呢。不久,收了老板cc信, 给 HR 的
。说 RA resign 了 and quote due
to 'difficulty' to work with me。
***
To make a long story short: the situation is now she is like a changed
person now, talking to me softly (vs.
previous always yells and stares at me) and report to me or contact me each
day, ect...
For my side, it is a bit too late for her final change. I will still treat
her professionally as I did over the
years, but I want to move on. For the sake that we were friends before, I
feel 得饶人处且饶人 and have no
regret after being mistreated by her for so many years I still at the last
min saved her job.
Lesson:
When being mistreated,
1.try to find your goal: if it is you want to be on upper hand or it is you
want to make a team mate, no
matter how poorly she performs--- if you consider you could get her to help
you to save youself 2 hours
per day, then it worth the effort to bring her in, ect
2. quote from a webpage: 'Never fight with morons, they will drag you to
their level and beat you with thier
experience
3. Be professional and treat her as if you'd treat any other co-workers. If
you feel worth it, collect the
eveidence
4. The major lesson #1:人际关系真的比science复杂。永远不应,不要与工作,周
围人(other than your best
friend) 讲,即使你是想‘求教’。When the words spread, you never know what
people would say 'What xxx
Said to xxx about xxx'.
5。The major lesson #2: I learned: Respect is what you want to gain in
work place, not friendship or
good -relations as what I did before life give me the hard lesson.
6. 做人要有底线才不被人欺:无数次,她说 'I am working on your carreer and
not even mine, ect..' At the time,
I should have told her 'she is not working on my career, she is working for
her job.' Indeed, not doing
postdoc, is not an option for me。 It's my job and that's it. // At those
days, I simply feel I really don't want
to sink myself that low to argue with her 'why she is there working with me.
'
******
Grad school,与三个中国RA共事。一直对中国RA很感激。也是造成薄厚期,一直不相
信I may indeed dealing with
a very different and difficult person.
Anyhow, I believe lots of Chinese postdocs have been in training all the
time and don't have much
experience in work-place relationship. Especially those like me, thinking it
is a purely a transient training
phase and don't want to get involve in stupid fightings.
就到这吧,如今发出来希望对大家有帮助。PS:认为在实验室久了,发好文了,就能让
关系好的,因人而异吧。久,你
永远不会比她久呀。
n******7
发帖数: 12463
2
中英文混得太均匀了,好累
话说你用什么输入法?
c*******e
发帖数: 4642
3
同看得好累
文中几处指代不清,表达含糊
最后一看还有那么长的英文,我直接quit了。。。

【在 n******7 的大作中提到】
: 中英文混得太均匀了,好累
: 话说你用什么输入法?

d********a
发帖数: 1852
4
你自己这样中英混杂,不累?

出入

【在 p*******7 的大作中提到】
: 一段时间前,本想‘第一次版上发贴,真心求教’,写了下文。后真的烦不愿再提。今
: 天读‘中国RA的文,以及回复,希望
: 分享我的’drama':
: 长话短说,RA:发 IF 》12 的一作时我还在本科,不知’科研‘何物。我, 薄厚前活泼
: 开朗,心直口快(现在,真的知道是
: 大毛病),喜欢宏观的可以见效果的。薄厚4年了,文章去工业界多了(朋友言),留学
: 术界又差着。‘人生的九曲湾
: 中’放弃追梦. 博后到了距家最近的Lab, 虽然lab focus 与我的Ph.D. training有出入
: 与此RA曾为朋友,老板3 年前发现课题有潜力, assign 此 RA, 非大陆的。我还开心
: ,不是因有RA,而是因为是朋

D*a
发帖数: 6830
5
其实我还是看完了的,但是看大家都在说中英文问题,我也加一句吧
楼主连俯拾即是都会用,怎么连“虽然lab focus 与我的Ph.D. training有出入”都要
中英文混着写?
还有“至此,我仍认为我可以give it a last try. 停了实验,面对面恳谈, 无果。”
i feel好confusing呀
s******r
发帖数: 2876
6
你太搞笑了。
不过他应该用的是搜狗拼音,中英混输都没有问题。

其实我还是看完了的,但是看大家都在说中英文问题,我也加一句吧
楼主连俯拾即是都会用,怎么连“虽然lab focus 与我的Ph.D. training有出入”都要
中英文混着写?
还有“至此,我仍认为我可以give it a last try. 停了实验,面对面恳谈, 无果。”
i feel好confusing呀

【在 D*a 的大作中提到】
: 其实我还是看完了的,但是看大家都在说中英文问题,我也加一句吧
: 楼主连俯拾即是都会用,怎么连“虽然lab focus 与我的Ph.D. training有出入”都要
: 中英文混着写?
: 还有“至此,我仍认为我可以give it a last try. 停了实验,面对面恳谈, 无果。”
: i feel好confusing呀

p*******7
发帖数: 23
7
只当一段故事了。对有困惑的人, 或不知怎吗进入旋涡的人有助便好了。读了那个关
于中国RA的贴, 心有沏沏,或自己的
挣扎对别人有助,而已。
***
About the language:
If typing English saves time, why not? If switch back and forth saves even
more time, why not? 谁让英语不是咱
母语,而又在美实验室傻呆了那么多年呢。Type Chinese is indeed not easy,
especially trying to avoid 错别字。
好,不顶峰作案了。还是少讲话吧。

出入

【在 p*******7 的大作中提到】
: 一段时间前,本想‘第一次版上发贴,真心求教’,写了下文。后真的烦不愿再提。今
: 天读‘中国RA的文,以及回复,希望
: 分享我的’drama':
: 长话短说,RA:发 IF 》12 的一作时我还在本科,不知’科研‘何物。我, 薄厚前活泼
: 开朗,心直口快(现在,真的知道是
: 大毛病),喜欢宏观的可以见效果的。薄厚4年了,文章去工业界多了(朋友言),留学
: 术界又差着。‘人生的九曲湾
: 中’放弃追梦. 博后到了距家最近的Lab, 虽然lab focus 与我的Ph.D. training有出入
: 与此RA曾为朋友,老板3 年前发现课题有潜力, assign 此 RA, 非大陆的。我还开心
: ,不是因有RA,而是因为是朋

n********k
发帖数: 2818
8
Comfort! what an experience...I think many of our fellow postdocs have
kind of similar experiences...I still don't quite figure out ways to deal
with it as we as postdoc don't have any authority and we are the lowest rank
in a lab...I try to be cheerful and grateful, say good things and sometimes
bribe with nice words and parties etc...After all, for them it is a job or
a way of making money, and for us, it is a career...it is different and I
try to be more understanding on that and lower my expectation...
On English, I share the same feeling, it takes forever to write in Chinese...It is bbs, why should we care whatever...that's why I am not changing any despite so many cutie/smarties have made fun of my poor English again and again, and again...I bet they would feel silly about themselves before I would ever change...

【在 p*******7 的大作中提到】
: 只当一段故事了。对有困惑的人, 或不知怎吗进入旋涡的人有助便好了。读了那个关
: 于中国RA的贴, 心有沏沏,或自己的
: 挣扎对别人有助,而已。
: ***
: About the language:
: If typing English saves time, why not? If switch back and forth saves even
: more time, why not? 谁让英语不是咱
: 母语,而又在美实验室傻呆了那么多年呢。Type Chinese is indeed not easy,
: especially trying to avoid 错别字。
: 好,不顶峰作案了。还是少讲话吧。

p*******7
发帖数: 23
9
特累。但是坚持下来了。因为事件发生在美国,希望给美国的中国薄厚提供过来人的经
历以及美国research insititutes
HR 及老板的处理的原形。
谢谢关心啦。

【在 d********a 的大作中提到】
: 你自己这样中英混杂,不累?
:
: 出入

p*******7
发帖数: 23
10
我刚来美国的时候,和你一样‘一看还有那么长的英文,我直接quit了’。呵呵。后来
不得不箕斗米折腰,要不然考试那不了
B=奖学金没了。
你比我肯定smart很多。
人,尤到了美国,就尽量open一些,包容些?
要过节了,真的没想累别人。

【在 c*******e 的大作中提到】
: 同看得好累
: 文中几处指代不清,表达含糊
: 最后一看还有那么长的英文,我直接quit了。。。

相关主题
如果genotyping结果发现一个SNP不符合HWE说明啥?一个GWAS genotype imputation的问题
wierd genotyping, and need help各位师弟师妹,到发考题版来学习一下吧
genotype 的价格Help: Failure of Red recombineering retrieve
进入Biology版参与讨论
p*******7
发帖数: 23
11
谢谢斑竹的包子。
祝大家过节好。又长大一岁:工作,待人处事, 中英文水平,都成长。

【在 p*******7 的大作中提到】
: 只当一段故事了。对有困惑的人, 或不知怎吗进入旋涡的人有助便好了。读了那个关
: 于中国RA的贴, 心有沏沏,或自己的
: 挣扎对别人有助,而已。
: ***
: About the language:
: If typing English saves time, why not? If switch back and forth saves even
: more time, why not? 谁让英语不是咱
: 母语,而又在美实验室傻呆了那么多年呢。Type Chinese is indeed not easy,
: especially trying to avoid 错别字。
: 好,不顶峰作案了。还是少讲话吧。

O******e
发帖数: 4845
12
应该是
i feel好confused呀
^_^

【在 s******r 的大作中提到】
: 你太搞笑了。
: 不过他应该用的是搜狗拼音,中英混输都没有问题。
:
: 其实我还是看完了的,但是看大家都在说中英文问题,我也加一句吧
: 楼主连俯拾即是都会用,怎么连“虽然lab focus 与我的Ph.D. training有出入”都要
: 中英文混着写?
: 还有“至此,我仍认为我可以give it a last try. 停了实验,面对面恳谈, 无果。”
: i feel好confusing呀

z*r
发帖数: 35
13
看着太累了。看了一小半就放弃了
但是祝你新年愉快,一切好运
c*******e
发帖数: 4642
14
不是我open的问题,是你太计较了,连路人甲乙的看法都看得那么紧。
再说,我也不是刚来美国了,时间两个巴掌正好数过来。
我对我的酱油造成你的不愉快感到抱歉。要过年了,不要多想了,抚摸你下吧。

【在 p*******7 的大作中提到】
: 我刚来美国的时候,和你一样‘一看还有那么长的英文,我直接quit了’。呵呵。后来
: 不得不箕斗米折腰,要不然考试那不了
: B=奖学金没了。
: 你比我肯定smart很多。
: 人,尤到了美国,就尽量open一些,包容些?
: 要过节了,真的没想累别人。

W****C
发帖数: 1937
15
思维在中英间不停的跳跃, 看到一半的时候闪了腰的飘过
圣诞了 有多少要在实验室过节的一同哀悼吧
p*******7
发帖数: 23
16
When I finally determined to share my 'atypical' struggling in the lab
initiated by the sympathy of the other post, I believe my struggling would
be of help: In my early days, if I'd read a post like this, I may have a
better reality check that 'ridiculous things do happen rather than doubt
about myself'.
******
Final words
1. if you are new, the tech is over 10 years in the same lab--- try to deal
with the first thing that rubs you wrong at the site by ASKING QUESTIONS.
She's been there so long, she must have her preference, ect. It is better to
ask early than let it go, speculating why and let things accumulate and
eventually become a big deal. The other side would appreciate that way too.
// When the thing escalated, using English is considered as being
professional in the lab although both can speak Chinese. In my case, the RA'
s English is very strong. But still, English per se, simplify things a lot:
less small talks and less emotion ect. In addition,when the boss involves,
there is no need for the 'translation' therefore less distortion from either
side.
2. if you are going to be a new PI and looking for first several hire: skill
is one important thing but team spirit is even more.
"Everything you do is at the expense that you CAN NOT DO, right?"
For now, I will bow out and do not bother to 'force' people to see my point
le. :-) When you fall and feel the bumps, you will learn to avoid next time.
If you are wise (which does come along side with aging :-), you don't fall
and learn from others' mistakes and avoid bruise yourself.
Got to finish the work early so that I'd finally start the 'Happy holidays'.

【在 p*******7 的大作中提到】
: 只当一段故事了。对有困惑的人, 或不知怎吗进入旋涡的人有助便好了。读了那个关
: 于中国RA的贴, 心有沏沏,或自己的
: 挣扎对别人有助,而已。
: ***
: About the language:
: If typing English saves time, why not? If switch back and forth saves even
: more time, why not? 谁让英语不是咱
: 母语,而又在美实验室傻呆了那么多年呢。Type Chinese is indeed not easy,
: especially trying to avoid 错别字。
: 好,不顶峰作案了。还是少讲话吧。

e**y
发帖数: 423
17
靠,看得我老非常生气
中英文混杂的太多了,有时是一句中文接一句英文
有时一个句子里半句中文半句英文,有时又是中文句子里加英文词组或者几个单词
不仅如此,中文也不纯粹,白话和半文言的用法混杂
白话,古文,英文混成一团,看了没到一半就头大了
简直想把楼主拖出去踩几脚
j*****a
发帖数: 658
18
haha. 另外奇怪一下,为啥biology版随便回个贴斑竹就给钱?

【在 e**y 的大作中提到】
: 靠,看得我老非常生气
: 中英文混杂的太多了,有时是一句中文接一句英文
: 有时一个句子里半句中文半句英文,有时又是中文句子里加英文词组或者几个单词
: 不仅如此,中文也不纯粹,白话和半文言的用法混杂
: 白话,古文,英文混成一团,看了没到一半就头大了
: 简直想把楼主拖出去踩几脚

z******g
发帖数: 5
19
看的太痛苦了~
T**********t
发帖数: 1604
20
楼主give我back我的10 minutes。。。
C********5
发帖数: 194
21
不是中英文混杂的问题,是思维跳跃混乱的问题。
D*a
发帖数: 6830
22
我觉得楼主其实应该反思一下平常说话(即使全是中文或者全是英文)是不是也这么跳跃
一般来说,能把中英文混成这样不证明任何一种语言好,只能说试图保持一致的行文风
格对楼主来说还是需要费脑子的,所以在论坛上轻松发帖的时候楼主自然选择了轻松的
方式。因为楼主夹英文的地方并不仅仅是根据楼主所说,为了叙述对方的对话或者反应
,也有我上面举例子说的“虽然lab focus 与我的Ph.D. training有出入”这种完全没
有必要的情况
如果我没有说错的话,可以想象楼主的谈话技巧至少是需要认真锻炼的,尤其楼主作为
一个领导者(此任务的PI)如果对下属的谈话和布置任务是这样的谈话方式,是很
frustrating的--也是论坛上经常抱怨的,老板一天一百个idea,一半不管用的,一
半有反作用的。
1 (共1页)
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