A***i
发帖数: 50 | 1 Sorry that I cannot type Chinese on this computer.
I have an ion channel gene mutation which was found in an atrial
fibrillation patient. I made the mutated DNA. And in western blot experiment
, the protein level dramatically reduced. In transfected culture cells,
there was no current recorded for the ion using patch clamp.
I wonder what else I can do to make a story out of the mutation. Your help
is highly appreciated! |
A***i
发帖数: 50 | 2 Somebody help, please!
experiment
【在 A***i 的大作中提到】
: Sorry that I cannot type Chinese on this computer.
: I have an ion channel gene mutation which was found in an atrial
: fibrillation patient. I made the mutated DNA. And in western blot experiment
: , the protein level dramatically reduced. In transfected culture cells,
: there was no current recorded for the ion using patch clamp.
: I wonder what else I can do to make a story out of the mutation. Your help
: is highly appreciated!
|
m*p
发帖数: 226 | 3 You saw point mutation and you also see lower translation of the gene. You
also mean this is a dominant negative mutation. Kind of confusing. Which is
or are responsible for the phenotype?
Which ion channel is affected? Put your gene to the whole ion channel story
and make a story. |
A***i
发帖数: 50 | 4 Sorry that I did not say clearly.
If I transfect the mutant alone, see no current in electrophysiology. But if
I transfect the mutant with wt, see no diffence than wt alone. So it is not
dominant negative. But causing atrial fibrillation in human.
is
story
【在 m*p 的大作中提到】
: You saw point mutation and you also see lower translation of the gene. You
: also mean this is a dominant negative mutation. Kind of confusing. Which is
: or are responsible for the phenotype?
: Which ion channel is affected? Put your gene to the whole ion channel story
: and make a story.
|
