x*****7
发帖数: 58 | 1 For my study, I proposed that our protein would have higher stability when
treated with copper. So, I used
Cyclohexamide to inhibit the protein synthesis. By doing this, I want to
find a time point that this protein start
to degrade and then I will add copper to see whether copper would increase
this protein stability. But, the
problem is once cell treated with the Cyclohexamide, most of the cells start
to dye. When I harvest the survived
cell and purify the protein which is resolved on SDS-PAGE. But there is no
difference between the control and
Cyclohexamide treatment group.
I want to know is there any method other than Cyclohexamide treatment could
inhibit protein synthesis. Or is
there other good method to test protein stability?
Thank you! | o*p
发帖数: 177 | 2 try CD? or NMR?
start
【在 x*****7 的大作中提到】
: For my study, I proposed that our protein would have higher stability when
: treated with copper. So, I used
: Cyclohexamide to inhibit the protein synthesis. By doing this, I want to
: find a time point that this protein start
: to degrade and then I will add copper to see whether copper would increase
: this protein stability. But, the
: problem is once cell treated with the Cyclohexamide, most of the cells start
: to dye. When I harvest the survived
: cell and purify the protein which is resolved on SDS-PAGE. But there is no
: difference between the control and
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