m****1
发帖数: 179 | 1 3-Methyladenine
sigma买的 查说明书 说用DMF溶解
可是根本溶不了啊 27ug 加了36ul DMF 还加热 震荡了
还是无济于事
抓狂了
谁有什么好办法吗？ | m****1
发帖数: 179 | | m****1
发帖数: 179 | 3 没人用过吗？ 是PI3K inhibitor
3-MA 这么小众吗？
为啥溶不了啊？ | j********r
发帖数: 156 | 4 As to 3MA, it is hard to dissolve it. The following is the method I always
use to prepare 200 mM stock in water:
For the Sigma 3MA (M9281-100MG)
1. Heat a large amount of tap water (say 500-1000 ml) to around 70 celsius
degrees.
2. Heat 3.352 ml tissue culture-grade water in a tube (eg. the tube for
flow cytometry or the regular 15 ml conical tube), by sitting it
in the heated water.
3. Remove the whole stuff into a hood (the temp of the tap water can
sustain for a while w/o a heater)
4. Transfer the whole above 3 ml water into the sigma bottle, then
put the bottle in the water bath until all stuff is dissolved.
5. Make aliquot of 100 ul per 1.5 ml tube. For convenience, I always
transfer the dissolved solution back into the tube for aliquoting
while the tube is sitting in the water.
6. Store the aliquots in a freezer. Upon use, add 1.9 ml medium to get
the final conc. of 10 mM (or 3.9 ml to 5 mM). | m****1
发帖数: 179 | 5
always
celsius
Thank you sooooooooooooooomuch!
感动坏了 这么详细的protocal
谢谢mm！！！！！！！！！！！！@——@
kiss！！！！！！！！！！！！！！！！！！！
【在 j********r 的大作中提到】
: As to 3MA, it is hard to dissolve it. The following is the method I always
: use to prepare 200 mM stock in water:
: For the Sigma 3MA (M9281-100MG)
: 1. Heat a large amount of tap water (say 500-1000 ml) to around 70 celsius
: degrees.
: 2. Heat 3.352 ml tissue culture-grade water in a tube (eg. the tube for
: flow cytometry or the regular 15 ml conical tube), by sitting it
: in the heated water.
: 3. Remove the whole stuff into a hood (the temp of the tap water can
: sustain for a while w/o a heater)
|
|
