j*****d
发帖数: 787 | 1 http://www.nature.com/cr/journal/vaop/ncurrent/full/cr2011158a.
anybody has full access? thanks.
However, they did not provide evidence that miRNA168a directly
travels from rice grains to human/animal stomac to serum (whose serum????)
and, timely, survive to what extent. Their functional study (miRNA168a
targets LDRAP1) acctually is an in vitro manipulation.
Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of
cross-kingdom regulation by microRNA
Lin Zhang, Dongxia Hou, Xi Chen, Donghai Li, Lingyun Zhu, Yujing Zhang, Jing
Li, Zhen Bian, Xiangying Liang, Xing Cai, Yuan Yin, Cheng Wang, Tianfu
Zhang, Dihan Zhu, Dianmu Zhang, Jie Xu, Qun Chen, Yi Ba, Jing Liu, Qiang
Wang, Jianqun Chen, Jin Wang, Meng Wang, Qipeng Zhang, Junfeng Zhang, Ke Zen
and Chen-Yu Zhang
AbstractOur previous studies have demonstrated that stable microRNAs (miRNAs
) in mammalian serum and plasma are actively secreted from tissues and cells
and can serve as a novel class of biomarkers for diseases, and act as
signaling molecules in intercellular communication. Here, we report the
surprising finding that exogenous plant miRNAs are present in the sera and
tissues of various animals and that these exogenous plant miRNAs are
primarily acquired orally, through food intake. MIR168a is abundant in rice
and is one of the most highly enriched exogenous plant miRNAs in the sera of
Chinese subjects. Functional studies in vitro and in vivo demonstrated that
MIR168a could bind to the human/mouse low-density lipoprotein receptor
adapter protein 1 (LDLRAP1) mRNA, inhibit LDLRAP1 expression in liver, and
consequently decrease LDL removal from mouse plasma. These findings
demonstrate that exogenous plant miRNAs in food can regulate the expression
of target genes in mammals. | I***a
发帖数: 13467 | | c********b
发帖数: 363 | 3 读了全文,觉得最重要的生理条件下这些miRNAs是有功能的这一点没有被很好的support.
当然,也很难.文章都是那chinese的血清,得找不吃大米的人的血清才有系,不然连对照
都没有. : )
你要全文的话给个email.
of
Jing
【在 j*****d 的大作中提到】
: http://www.nature.com/cr/journal/vaop/ncurrent/full/cr2011158a.
: anybody has full access? thanks.
: However, they did not provide evidence that miRNA168a directly
: travels from rice grains to human/animal stomac to serum (whose serum????)
: and, timely, survive to what extent. Their functional study (miRNA168a
: targets LDRAP1) acctually is an in vitro manipulation.
: Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of
: cross-kingdom regulation by microRNA
: Lin Zhang, Dongxia Hou, Xi Chen, Donghai Li, Lingyun Zhu, Yujing Zhang, Jing
: Li, Zhen Bian, Xiangying Liang, Xing Cai, Yuan Yin, Cheng Wang, Tianfu
| j*****d
发帖数: 787 | 4 如果能证明在mouse中有功能都足够了。还有，有没有更直接的证明这些mir定位于单细
胞内？
support.
★ 发自iPhone App: ChineseWeb - 中文网站浏览器
【在 c********b 的大作中提到】
: 读了全文,觉得最重要的生理条件下这些miRNAs是有功能的这一点没有被很好的support.
: 当然,也很难.文章都是那chinese的血清,得找不吃大米的人的血清才有系,不然连对照
: 都没有. : )
: 你要全文的话给个email.
:
: of
: Jing
| c********b
发帖数: 363 | 5 对mammal系统不懂,不敢评价.你自个去瞅瞅.
抑制蛋白表功能都是构建过表达看到的,这个不要说证明,suggest生理功能都很难.
【在 j*****d 的大作中提到】
: 如果能证明在mouse中有功能都足够了。还有，有没有更直接的证明这些mir定位于单细
: 胞内？
:
: support.
: ★ 发自iPhone App: ChineseWeb - 中文网站浏览器
| s******y
发帖数: 28562 | 6 我有，把email address 给我吧
我粗粗的看了一下，数据还是挺多的，文章也比较完整。
当然他们没有特别直接的实验来证明他们测到的miRNA168a是直接来自于食物的
（比方说放射性标记一下那个miRNA168a），但是有一大堆间接证据，比方说进食米饭
之后这个东西在血清里的浓度就上升，进食对照食品之后不上升，但是这个有一个顾虑
就是不能排除是代谢反应。不过，话说回来，这个mrRNA168a应该是不可能来源于人的
基因组的吧？
of
Jing
【在 j*****d 的大作中提到】
: http://www.nature.com/cr/journal/vaop/ncurrent/full/cr2011158a.
: anybody has full access? thanks.
: However, they did not provide evidence that miRNA168a directly
: travels from rice grains to human/animal stomac to serum (whose serum????)
: and, timely, survive to what extent. Their functional study (miRNA168a
: targets LDRAP1) acctually is an in vitro manipulation.
: Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of
: cross-kingdom regulation by microRNA
: Lin Zhang, Dongxia Hou, Xi Chen, Donghai Li, Lingyun Zhu, Yujing Zhang, Jing
: Li, Zhen Bian, Xiangying Liang, Xing Cai, Yuan Yin, Cheng Wang, Tianfu
| l*****e
发帖数: 138 | 7 大概看了一下，数据很多．不过我第一反应是，为什么他们要用HepG2 这个肝癌细胞株
做体外试验呢？
of
Jing
【在 j*****d 的大作中提到】
: http://www.nature.com/cr/journal/vaop/ncurrent/full/cr2011158a.
: anybody has full access? thanks.
: However, they did not provide evidence that miRNA168a directly
: travels from rice grains to human/animal stomac to serum (whose serum????)
: and, timely, survive to what extent. Their functional study (miRNA168a
: targets LDRAP1) acctually is an in vitro manipulation.
: Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of
: cross-kingdom regulation by microRNA
: Lin Zhang, Dongxia Hou, Xi Chen, Donghai Li, Lingyun Zhu, Yujing Zhang, Jing
: Li, Zhen Bian, Xiangying Liang, Xing Cai, Yuan Yin, Cheng Wang, Tianfu
|
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