m******5
发帖数: 1383 | 1 I happened to be reading a review article published 1 year ago talking about
that actin play a very important role inside cell nuclear, by recruting
Nuclearsome remodeling complex or stablizing pre-mRNA.
But there isn't any NLS harbored on actin, and it has been proved that actin
can not migrate into cell nuclear on their own. So, how come a little
amount of actin leaking into cell nuclear can have so significant function?
On the other hand, did anyone ffind that different isoforms of actin have
different cytoplasmic/nuclear distribution rate?
I am deeply intrigued by that %$%$ |
s******y
发帖数: 28562 | 2 核内的actin 的问题其实目前还不是特别清楚。
一般来说，如果是那种特别大的核（比方说卵细胞的核），往往是有
atin的.至于怎么进去的？目前大家也不是很清楚
about
actin
【在 m******5 的大作中提到】
: I happened to be reading a review article published 1 year ago talking about
: that actin play a very important role inside cell nuclear, by recruting
: Nuclearsome remodeling complex or stablizing pre-mRNA.
: But there isn't any NLS harbored on actin, and it has been proved that actin
: can not migrate into cell nuclear on their own. So, how come a little
: amount of actin leaking into cell nuclear can have so significant function?
: On the other hand, did anyone ffind that different isoforms of actin have
: different cytoplasmic/nuclear distribution rate?
: I am deeply intrigued by that %$%$
|
m******5
发帖数: 1383 | 3 就是说一部分人不相信，一部分人不光相信而且把功能做得很细了。。。。。
【在 s******y 的大作中提到】
: 核内的actin 的问题其实目前还不是特别清楚。
: 一般来说，如果是那种特别大的核（比方说卵细胞的核），往往是有
: atin的.至于怎么进去的？目前大家也不是很清楚
:
: about
: actin
|
y****z
发帖数: 27 | 4 isn't actin associtaed with microtuble ? since mt is there, why actin can't
about
actin
【在 m******5 的大作中提到】
: I happened to be reading a review article published 1 year ago talking about
: that actin play a very important role inside cell nuclear, by recruting
: Nuclearsome remodeling complex or stablizing pre-mRNA.
: But there isn't any NLS harbored on actin, and it has been proved that actin
: can not migrate into cell nuclear on their own. So, how come a little
: amount of actin leaking into cell nuclear can have so significant function?
: On the other hand, did anyone ffind that different isoforms of actin have
: different cytoplasmic/nuclear distribution rate?
: I am deeply intrigued by that %$%$
|
c******g
发帖数: 49 | 5 似乎actin进核是挺普遍的吧，我自己也观察到过，但是也不知道机理以及作用。听过
一个报告，似乎actin进核同某种病毒在核内复制相关。 |
c********r
发帖数: 189 | 6 是可以进核的，而且好像进核的还不少，我们实验室有人做过cell fractionation，确
实都有，但是我不知道有啥功能 |
c******g
发帖数: 49 | 7 请问楼上XD， 你们cell fractionation怎么做的？能否分享你的protocol？谢谢 |
s******y
发帖数: 28562 | 8 actin 和microtube 没有直接联系，而必须通过一些其他蛋白的介导。
而microtube 在大部分情况下并不在核内形成，除非某些特定的细胞，特定的场合。
你可能是误会把细胞分裂时候的染色体和microtube 的关系吧？
其实细胞分裂的时候核膜一般就解散了，或者通透了，所以和平常时候的核不是一回事。
t
【在 y****z 的大作中提到】
: isn't actin associtaed with microtuble ? since mt is there, why actin can't
:
: about
: actin
|
b******s
发帖数: 1089 | 9 为什么核膜重新形成时不可以占有部分MT和actin pool呢？
事。
【在 s******y 的大作中提到】
: actin 和microtube 没有直接联系，而必须通过一些其他蛋白的介导。
: 而microtube 在大部分情况下并不在核内形成，除非某些特定的细胞，特定的场合。
: 你可能是误会把细胞分裂时候的染色体和microtube 的关系吧？
: 其实细胞分裂的时候核膜一般就解散了，或者通透了，所以和平常时候的核不是一回事。
:
: t
|
m******5
发帖数: 1383 | 10 这个很有意思，其实在核解聚的时候很多本来应该是cytoplasmic分布的蛋白都会在核
里检到
但核膜重新形成后又该干嘛干嘛了
【在 b******s 的大作中提到】
: 为什么核膜重新形成时不可以占有部分MT和actin pool呢？
:
: 事。
|
|
|
s******y
发帖数: 28562 | 11 不清楚，可能会被选择性的踢出去吧。
有些细胞核在某些情况下也可以有tubulin的，但是功能未明。
至于核内的actin，大部分研究认为和染色体结构以及transcription 有功能性
的关系。但是机理未明。
【在 b******s 的大作中提到】
: 为什么核膜重新形成时不可以占有部分MT和actin pool呢？
:
: 事。
|
h****n
发帖数: 2552 | |
m******5
发帖数: 1383 | 13 Nuclear Functions of Actin
Neus Visa, and Piergiorgio Percipalle
【在 h****n 的大作中提到】
: 楼主能否把文献的title列出来
|
n****n
发帖数: 434 | 14 some proteins (residing in the cytoplasm in non-mitotic cells) can be
wrapped into the nucleus at/after mitotic exit. |
g*********5
发帖数: 2533 | 15 反正我用kit fraction cell，核里总是有actin，还不少。 |
m******5
发帖数: 1383 | 16 I don't think this is always the case:
at least they should manifest some important function to be stabilized
inside cell nuclear.
A lot of other proteins can be wrapped into the nucleus but soon been
degraded
【在 n****n 的大作中提到】
: some proteins (residing in the cytoplasm in non-mitotic cells) can be
: wrapped into the nucleus at/after mitotic exit.
|
