g******1
发帖数: 295 | 1 搞计算机的，不太懂生物，rrdw下rrdw下sequencing中primer和adaptor的区别？
primer和adaptor分别什么作用呢？谢谢 |
s******r
发帖数: 91 | 2 adapter加在靶标序列上为测序primer提供锚定位点 |
g******1
发帖数: 295 | 3 比如454出来的每个read中，
1. 有adaptor序列吗？还是不对adaptor进行测序？
2. 有primer序列吗？还是不对primer进行测序？
【在 s******r 的大作中提到】
: adapter加在靶标序列上为测序primer提供锚定位点
|
R****n
发帖数: 708 | 4 Generally speaking adaptor and primer are all oligo nucleotide sequences.
The difference lies in their roles in a 2nd generation sequencing reaction.
Adaptor is used for ligating the primer to the target sequences(usually for
DNA,RNA,miRs).Certain sequences are more efficient for ligation reaction.
Sometimes there will be a barcode sequences between adaptor and primer to
identify each sample when multiple samples are sequenced.common primer pair
is used for amplify a huge pool of ligated sequences.
【在 g******1 的大作中提到】
: 比如454出来的每个read中，
: 1. 有adaptor序列吗？还是不对adaptor进行测序？
: 2. 有primer序列吗？还是不对primer进行测序？
|
g******1
发帖数: 295 | 5 请问下什么是common primer pair?
谢谢
.
for
pair
【在 R****n 的大作中提到】
: Generally speaking adaptor and primer are all oligo nucleotide sequences.
: The difference lies in their roles in a 2nd generation sequencing reaction.
: Adaptor is used for ligating the primer to the target sequences(usually for
: DNA,RNA,miRs).Certain sequences are more efficient for ligation reaction.
: Sometimes there will be a barcode sequences between adaptor and primer to
: identify each sample when multiple samples are sequenced.common primer pair
: is used for amplify a huge pool of ligated sequences.
|
R****n
发帖数: 708 | 6 common primer pair is the sequencing primer pair. Depending on the library
construction, there are some variations. You can imagine that genomic dna is
fragmented in a range of 100-500bp. Only one pair of primer is added to
their 5'and 3' end.
【在 g******1 的大作中提到】
: 请问下什么是common primer pair?
: 谢谢
:
: .
: for
: pair
|
g******1
发帖数: 295 | 7 是所有fragments都用同一对primer对吗?
是不是加在5'端的叫forward primer，3'的叫reverse primer？
16S rRNA 测序时，我还看到一个概念叫universal primer,请问什么是什么意思？
谢谢！
is
【在 R****n 的大作中提到】
: common primer pair is the sequencing primer pair. Depending on the library
: construction, there are some variations. You can imagine that genomic dna is
: fragmented in a range of 100-500bp. Only one pair of primer is added to
: their 5'and 3' end.
|
