J******9
发帖数: 736 | 1 i need this suppliy in my work,but i dont know the name of this in english
and where to buy it. Doese anybody know it?
thanks |
d**********r
发帖数: 36 | |
J******9
发帖数: 736 | |
J******9
发帖数: 736 | 4 sorry, it is not for cell-culture purpose,just for the immunostain...it is
made of glass.....
染色板，孔底拱顶形，节约抗体，利于更换液体; |
s******y
发帖数: 28562 | 5 看半天没看出该怎么用这个东西。。。
是不是要把一个很小的盖玻片倒扣着放进槽里面用的？
【在 J******9 的大作中提到】
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g*r
发帖数: 2116 | 6 spot plate
这玩意儿这边死贵死贵啊
http://www.fishersci.com/ecomm/servlet/productimagesview?catalo
【在 J******9 的大作中提到】
: i need this suppliy in my work,but i dont know the name of this in english
: and where to buy it. Doese anybody know it?
: thanks
|
J******9
发帖数: 736 | 7 i used it a lot when i was in china...it is a common stuff in the lab...i
just dont know its name in english |
v*********d
发帖数: 382 | |
J******9
发帖数: 736 | 9 Fisher Scientific
Pack of 6 for $309.59...why they dont provide a single package? i just
need one~!
【在 g*r 的大作中提到】
: spot plate
: 这玩意儿这边死贵死贵啊
: http://www.fishersci.com/ecomm/servlet/productimagesview?catalo
|
f*******o
发帖数: 150 | 10 we call it "watch Glass". Good Luck.
【在 J******9 的大作中提到】
: i need this suppliy in my work,but i dont know the name of this in english
: and where to buy it. Doese anybody know it?
: thanks
|
|
|
J******9
发帖数: 736 | 11 yes, the glass one is better than Glazed/White Porcelain Plate..the price is
also double~!
anyway, i am so glad that i always could find the answers from mitbbs
thanks you all |
s******y
发帖数: 28562 | |
x**w
发帖数: 112 | |
J******9
发帖数: 736 | 14 it is normally used for the immuno-stain....put the sectioned tissues in the
wells,and treat them like the normal mounting/floating sections...
Advantage:
1,u could very easily treat the frozen tissues in the well,without running
into the risk of tissue damage,
2, also u could save a little bit more of antibody working solution....add ~
100 ul for each well,
3,also the solution is not easy to evaporate in the humid chamber
4, personally,i think it is better than the frozen mounting slides,which
usually have lots of folded sections or damaged regions observed under the
microscope,which is very frustrating in deed. |
g*r
发帖数: 2116 | 15 这玩意儿就是玻璃上有些凹槽 可以盛点溶液... 做个dissection/staining啥的有时候
还挺方便 实验室里很多 没想到还挺贵的
【在 s******y 的大作中提到】
: 能不能讲讲这个东西怎么个用的？
|
J******9
发帖数: 736 | 16 and also, if the primary Ab is expensive, what i do is to harvest the used
antibody for additional 1-2 times...it works well actually.. |
s******y
发帖数: 28562 | 17 哦！我们都是把抗体直接滴在 parafilm上面，然后上面倒扣一个塑料培养皿。
【在 g*r 的大作中提到】
: 这玩意儿就是玻璃上有些凹槽 可以盛点溶液... 做个dissection/staining啥的有时候
: 还挺方便 实验室里很多 没想到还挺贵的
|
