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AAN: Stem Cells Show Promise in Mouse Model of ALS
THURSDAY, Jan. 10 (HealthDay News) -- Implantation of a neural stem cell (
NCS) fraction of induced pluripotent stem cells (iPSCs) shows promise in a
mouse model of amyotrophic lateral sclerosis (ALS), according to a study
released in advance of its presentation at the annual meeting of the
American Academy of Neurology, which will be held from March 16 to 23 in San
Diego.
Stefania Corti, M.D., Ph.D., from the University of Milan, and colleagues
used a non-viral, non-integrating method based on the expression of
reprogramming factors with episomal vectors to generate iPS cell lines from
human skin fibroblasts. A protocol to promote NSC fate was used to
differentiate iPSCs. Based on their high ALDH activity and low side scatter
(ADLHhiSSClo), a primitive NSC fraction was isolated. iPSC-purified NSCs
were transplanted either intrathecally or by systemic intravenous injection
into SOD1G93A mice (mouse model of ALS).
The researchers found that ALDH(hi)SSC(lo) NSCs from iPSCs are self-renewing
and multipotent. In vitro, they were able to differentiate into the three
neuroectodermal lineages. NSCs (both intrathecally and systemically grafted)
migrated into the parenchyma and engrafted the host spinal cord, where they
expressed neuronal precursors- and neuronal mature-specific markers. In the
mouse model of ALS, cell transplantation prolonged disease duration and
lifespan, promoted survival of motor neurons, and improved neuromuscular
function.
"ALS is a fatal, progressive, degenerative disease that currently has no
cure. Stem cell transplants may represent a promising avenue for effective
cell-based treatment for ALS and other neurodegenerative diseases," Corti
said in a statement. |
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