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发帖数: 1281 | 1 Job ID: GF2013A001
Posting Title: Research Scientist/Research Associate/Bacterial Geneticist/
Fermentation Scientist
Brief Introduction of Gate Fuels Inc.: “Imagination, Innovation,
Implementation --- Enabled Technologies for a Green Clean World”
Gate Fuels Inc. is a research start-up firm based in Blacksburg, VA, with
the goal of developing microbial platform technologies that enable low-cost
production of biochemicals and biofuels. The platform is based on the well-
studied Gram-positive model strain Bacillus subtilis. This microbe has the
capacity to convert cellulosic biomass directly to value-added biofuels and
biochemicals in a single step, called consolidated bioprocessing (CBP). The
co-founders of Gate Fuels Inc. have worked on Bacillus subtilis and CBP for
more than 25 years (combined experience).
Position Description:
Gate Fuels Inc. is seeking a talented, motivated, and creative scientist to
join the microbial consolidated bioprocessing team. Gate Fuels Inc. was
funded by U.S. National Science Foundation (NSF) and Department of Energy (
DOE) to develop a new ultra-low-cost platform for the production of
renewable biochemicals directly from pretreated lignocellulosic biomass in a
single step by using a novel recombinant cellulolytic Bacillus subtilis
strain. We have validated our proof-of-concept in Phase I programs are now
entering the Phase II programs for the further development and enhancement
of our proprietary technologies. Lignocellulosic biomass is the most
abundant natural renewable resource, which has great potential in the
production of valuable biocommodities for both short- and long-term
sustainability. However, the process for converting non-food lignocellulosic
material into biocehmicals is not feasible yet due to the high cost of
cellulase involved in cellulose hydrolysis and also to the use of costly
laboratory culture media. Through the use of systematic genetic engineering,
metabolic engineering and synthetic biology approaches, we will convert non
-cellulose-utilizing B. subtilis to an efficient lignocellulose utilizer and
to produce desired biochemicals with high yield, titer and productivity.
As a scientist within the R&D team, you will apply your research and
development skills on cellulase engineering, genetic engineering, metabolic
engineering and fermentation of Bacillus subtilis strains, and the
characterization of newly engineered strains. The individual will join a
group of interactive scientists and should have strong communication skills.
Salary, fringe benefits, and stock option will be commensurate with
experience and qualifications.
Specific responsibilities:
Perform and complete the tasks assigned by the group leader and generate a
final report;
Design and construct hybrid DNA constructs in E. coli or Bacillus subtilis;
Cloning, expression and characterization of recombinant cellulases in
Bacillus subtilis and E. coli;
Improve the performance (expression, secretion and specific activity) of
cellulases and cellulosomes by directed evolution and rational design;
Perform gene insertion and knockout in Bacillus subtilis and the new strain
characterization;
Perform the small and large scale fermentations of the engineered B.
subtilis strains;
Conduct trouble shooting and implement technology solutions;
Communicate research progress and objectives clearly, frequently, and
effectively.
Minimum Requirements:
M.S./Ph.D. or equivalent in microbiology, molecular biology, biochemistry,
biomolecular engineering, fermentation or a related field and 2+ years of
laboratory experience in the related areas;
Strong motivation to learn new technologies and to implement new ideas;
Be able to initiate projects independently with minimum supervision;
Proficient in PCR technology, DNA sequence analysis, primer and gene design,
engineering and analysis;
Be familiar with bacterial transformation technologies and homologous
recombination;
Be familiar with DNA cloning, protein expression and purification
technologies;
Strong work ethic and enjoy working in a fast-paced, team-oriented
environment;
Ability to follow instructions and company policies and compliance with
safety procedures;
Demonstrated track record of problem solving and innovation;
Excellent organization and time management skills;
Experience with HPLC and GC analysis is preferred;
Experience with small or large scale fermentation is preferred;
Past experience with Bacillus subtilis is preferred;
Past experience with cellulase is preferred;
Be legally authorized to work in the US without sponsorship.
Application deadline: March 31, 2013.
To Apply: Please send your application (cover letter and resume) to careers@
gatefuels.com. | l*********1
发帖数: 351 | | n******t
发帖数: 1281 | 3 看错了吧
你跑去拉斯维加斯了啊
【在 l*********1 的大作中提到】
: 恭喜张教授
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