h******y
发帖数: 351 | 1 终于克隆人了。
Human Embryonic Stem Cells Derived by Somatic Cell Nuclear Transfer
Cell, 15 May 2013
doi:10.1016/j.cell.2013.05.006
Reprogramming somatic cells into pluripotent embryonic stem cells (ESCs) by
somatic cell nuclear transfer (SCNT) has been envisioned as an approach for
generating patient-matched nuclear transfer (NT)-ESCs for studies of disease
mechanisms and for developing specific therapies. Past attempts to produce
human NT-ESCs have failed secondary to early embryonic arrest of SCNT embryo
s. Here, we identified premature exit from meiosis in human oocytes and subo
ptimal activation as key factors that are responsible for these outcomes. Op
timized SCNT approaches designed to circumvent these limitations allowed der
ivation of human NT-ESCs. When applied to premium quality human oocytes, NT-
ESC lines were derived from as few as two oocytes. NT-ESCs displayed normal
diploid karyotypes and inherited their nuclear genome exclusively from paren
tal somatic cells. Gene expression and differentiation profiles in human NT-
ESCs were similar to embryo-derived ESCs, suggesting efficient reprogramming
of somatic cells to a pluripotent state. | o**4
发帖数: 35028 | | o**4
发帖数: 35028 | | f*******o
发帖数: 150 | 4 看看发表的速度，几乎没有修改。
Received: April 30, 2013
Revised: May 3, 2013
Accepted: May 3, 2013
Published: May 15, 2013 | s*****w
发帖数: 27 | | l**********1
发帖数: 5204 | 6 Shame on Cell Senior Editors if it was retracted later
from author/s misconduct by PS WB or GFP figure/s...
http://www.mitbbs.com/article_t/Biology/31809251.html
【在 s*****w 的大作中提到】
: The paper is challenged and Cell is investigating now.
: http://pubpeer.com/publications/F0CFE0360002C25DC0BEFE28987D70
: http://news.sciencemag.org/scienceinsider/2013/05/cell-investig
| a*********n
发帖数: 2526 | | s*****w
发帖数: 27 | 8 Response from Cell:
"Questions have been raised in PubPeer, a website where people can make
anonymous comments about published papers, about some of the figures in this
paper. Based on our own initial in-house assessment of issues raised and in
initial discussions with the authors, it seems that there were some minor
errors made by the authors when preparing the figures for initial submission
. While we are continuing discussions with the authors, we do not believe
these errors impact the scientific findings of the paper in any way."
"A number of comments about these errors in articles and blogs have drawn
connections to the speed of the peer review process for this paper. Given
the broad interest, importance, anticipated scrutiny of the claims of the
paper and the preeminence of the reviewers, we have no reason to doubt the
thoroughness or rigor of the review process. The comparatively rapid
turnaround for this paper can be attributed to the fact that the reviewers
graciously agreed to prioritize attention to reviewing this paper in a
timely way. It is a misrepresentation to equate slow peer review with
thoroughness or rigor or to use timely peer review as a justification for
sloppiness in manuscript preparation."
Emilie Marcus, Editor-in-Chief of Cell and the Cell editorial team
Reports from Nature:
http://www.nature.com/news/stem-cell-cloner-acknowledges-errors |
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