h*****c
发帖数: 505 | 1 要多少钱啊？
都要买些什么东西啊
我们这边做一些表面处理的试验，想检测一下处理后材料的生物相容性什么的，准备做
一下cell culture study, 下面是从文献中找到的试验方法
Cell culture experiments were performed using mouse pre-osteoblast
MC3T3-E1 subclone 14 and rat fibroblast Rat1 cell lines provided by
American Type Culture Collection (ATCC, Manassas, VA, USA). Disks
of the titanium substrates with a diameter of one centimeter were placed in
24-well culture plates and incubated in alpha minimum essential medium
(a-MEM, Invitrogen Corporation, USA) and Dulbecco’s modified Eagle’s
medium supplemented with 10% fetal bovine serum, 100 U/ml penicillin
containing 100mg/ml streptomycin at 371C in a humidified atmosphere of
5% CO2 and 95% air. Controlled experiments were conducted on
conventional culture well plates for each set of experiments
谢谢大家！ | s******s
发帖数: 13035 | 2 你们实验室没人做这个的就不要试了，找人合作吧。
in
’s
【在 h*****c 的大作中提到】
: 要多少钱啊？
: 都要买些什么东西啊
: 我们这边做一些表面处理的试验，想检测一下处理后材料的生物相容性什么的，准备做
: 一下cell culture study, 下面是从文献中找到的试验方法
: Cell culture experiments were performed using mouse pre-osteoblast
: MC3T3-E1 subclone 14 and rat fibroblast Rat1 cell lines provided by
: American Type Culture Collection (ATCC, Manassas, VA, USA). Disks
: of the titanium substrates with a diameter of one centimeter were placed in
: 24-well culture plates and incubated in alpha minimum essential medium
: (a-MEM, Invitrogen Corporation, USA) and Dulbecco’s modified Eagle’s
| l******a
发帖数: 3339 | 3 这些东西对于做生物的来说就是routine，piece of cake，对于你来说少说也够你折腾
小半年，而且根本没有意义，找人合作吧，或者让你们老板招个做生物的博后。
in
’s
【在 h*****c 的大作中提到】
: 要多少钱啊？
: 都要买些什么东西啊
: 我们这边做一些表面处理的试验，想检测一下处理后材料的生物相容性什么的，准备做
: 一下cell culture study, 下面是从文献中找到的试验方法
: Cell culture experiments were performed using mouse pre-osteoblast
: MC3T3-E1 subclone 14 and rat fibroblast Rat1 cell lines provided by
: American Type Culture Collection (ATCC, Manassas, VA, USA). Disks
: of the titanium substrates with a diameter of one centimeter were placed in
: 24-well culture plates and incubated in alpha minimum essential medium
: (a-MEM, Invitrogen Corporation, USA) and Dulbecco’s modified Eagle’s
| h*****c
发帖数: 505 | 4 好啊，谢谢！
【在 l******a 的大作中提到】
: 这些东西对于做生物的来说就是routine，piece of cake，对于你来说少说也够你折腾
: 小半年，而且根本没有意义，找人合作吧，或者让你们老板招个做生物的博后。
:
: in
: ’s
| h*****c
发帖数: 505 | 5 恩 谢谢啊
【在 s******s 的大作中提到】
: 你们实验室没人做这个的就不要试了，找人合作吧。
:
: in
: ’s
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