c*******0
发帖数: 190 | 1 http://www.nature.com/ncomms/2015/150915/ncomms9264/full/ncomms
Abstract
A major challenge in neuroscience is to reliably activate individual neurons
, particularly those in deeper brain regions. Current optogenetic approaches
require invasive surgical procedures to deliver light of specific
wavelengths to target cells to activate or silence them. Here, we
demonstrate the use of low-pressure ultrasound as a non-invasive trigger to
activate specific ultrasonically sensitized neurons in the nematode,
Caenorhabditis elegans. We first show that wild-type animals are insensitive
to low-pressure ultrasound and require gas-filled microbubbles to transduce
the ultrasound wave. We find that neuron-specific misexpression of TRP-4,
the pore-forming subunit of a mechanotransduction channel, sensitizes
neurons to ultrasound stimulus, resulting in behavioural outputs.
Furthermore, we use this approach to manipulate the function of sensory
neurons and interneurons and identify a role for PVD sensory neurons in
modifying locomotory behaviours. We suggest that this method can be broadly
applied to manipulate cellular functions in vivo. |
c*******0
发帖数: 190 | 2 Room for Magnetogenetics to get Nobel is shrinking... |
O********4
发帖数: 113 | 3 Cool. 不过这个是Trp channel , 尽管应用上和张类似，但机理上的新奇程度不如谢，
谢灿发现的有根本不同。即使是在磁场感应中，还有另一个TRP channel 也是有类似功
能（2008年发过Nature）。
不过这样看来，TRP channel 倒有可能得奖，最早克隆出来TRP 的Craig Montell 得奖
的可能性大了些。这样的话 johns hopkins 又丢了一个诺奖，可惜 。 |
s******8
发帖数: 2131 | 4 光、电、气味、空间等等的都出来了吗？气味的好像已经有了 |
r*s
发帖数: 2555 | |
c*******0
发帖数: 190 | 6 Optogenetics
Sonogenetics
Magnetogenetics
XXXgenetics...
【在 s******8 的大作中提到】
: 光、电、气味、空间等等的都出来了吗？气味的好像已经有了
|
k*****n
发帖数: 417 | 7 张生家要哭了。不过这和谢灿发现MagR不一样，谢的发现既有科学意义，应用前景也很
广阔。
【在 c*******0 的大作中提到】
: Room for Magnetogenetics to get Nobel is shrinking...
|
d******n
发帖数: 3836 | 8 gravitogenetics
【在 c*******0 的大作中提到】
: Optogenetics
: Sonogenetics
: Magnetogenetics
: XXXgenetics...
|
j******i
发帖数: 939 | 9 有意思的是这哥们同时在eLIFE发了一篇‘气味遗传学’ http://elifesciences.org/content/4/e10181
Circuit mechanisms encoding odors and driving aging-associated behavioral
declines in Caenorhabditis elegans
eLife digest
A sense of smell can help animals to find food and detect danger. Odor
molecules activate so-called olfactory neurons that relay signals to the
brain in the form of nerve impulses. This information is then processed, and
the appropriate response is triggered; for example, an animal might move
towards the smell of food, or away from the scent of a predator. But how can
the activity of olfactory neurons trigger the right behavioral response?
Leinwand et al. have now explored the activity of olfactory neurons in a
roundworm called C. elegans. The experiments revealed that a food odor
activated two olfactory neurons directly, and that each of these ‘primary’
neurons then in turn activated another ‘secondary’ olfactory neuron. This
communication between primary and secondary olfactory neurons was essential
for worms to respond to the food odor. Further experiments revealed that
the primary olfactory neurons send chemical signals, called
neurotransmitters and neuropeptides, to communicate with the secondary
neurons. Importantly, mutations that blocked this chemical signaling
prevented the worms from responding appropriately to the smell of food.
Aging animals, including people, often have impaired senses and can
therefore find it difficult to identify and respond to odors. Leinwand et al
. found that aged worms were no different. Further experiments suggested
that aging worms' responses to odor decline because the communication
between the primary and secondary olfactory neurons may be impaired with age
. When Leinwand et al. strengthened this communication it reversed the
effects of aging on the worms' sense of smell. Moreover, the experiments
also showed that an animal's performance on the odor task was correlated
with its longevity, such that the better performers also lived longer. A
challenge for the future is to understand the precise changes that occur at
early stages of aging to impair the sense of smell. Future studies could
also test if similar combinations of olfactory neurons are needed to trigger
certain behavioral responses to odors in young and old mammals. |
l********a
发帖数: 61 | 10 这个版上只剩下一群不搞生物的ID了么。。没看到那个microbubbles么，应用极其局限
好么，和magnetogenetics 根本就不是一个level的。。更别提用超声波的危害了。。 |
c*******0
发帖数: 190 | 11 Are you "搞生物的ID"?
更别提用超声波的危害了。。
【在 l********a 的大作中提到】
: 这个版上只剩下一群不搞生物的ID了么。。没看到那个microbubbles么，应用极其局限
: 好么，和magnetogenetics 根本就不是一个level的。。更别提用超声波的危害了。。
|
B***I
发帖数: 3 | 12
----------------------
Montell没希望的，TRP如果和炸药沾边，只可能是Julius。
不管是神经领域还是电生理领域，K通道的重要性只会在TRP之上而不在TRP之下。Lily
是第一个克隆出shaker Kv 通道的人，拿奖了吗？
======================
草根阿戈
======================
【在 O********4 的大作中提到】
: Cool. 不过这个是Trp channel , 尽管应用上和张类似，但机理上的新奇程度不如谢，
: 谢灿发现的有根本不同。即使是在磁场感应中，还有另一个TRP channel 也是有类似功
: 能（2008年发过Nature）。
: 不过这样看来，TRP channel 倒有可能得奖，最早克隆出来TRP 的Craig Montell 得奖
: 的可能性大了些。这样的话 johns hopkins 又丢了一个诺奖，可惜 。
|
