ik
发帖数: 167 | 1 This is the abstract:
The authors describe a highly sensitive colorimetric method for the
determination of ascorbic acid. It is based on the finding that nanosheets
of reduced graphene oxide that were functionalized with poly(sodium styrene
sulfonate) (PSS-rGO) possess peroxidase-like activity that catalyzes the
oxidization of the substrate 3,3',5,5'-tetramethylbenzidine (TMB) by H2O2 to
form a blue product that can be quantified by photometry or visually. If
ascorbic acid is present, a fraction of H2O2 will be consumed and hence less
blue product (with an absorption peak at 652 nm) will be formed. Ascorbic
acid can be quantified by photometry in the 0.8 to 60 μM concentration
range with a 0.15 μM detection limit. It was successfully applied to the
determination of ascorbic acid in vitamin C tablets and orange juice.
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