w*****9
发帖数: 14 | 1 Recently I am doing drug uptaking experiments. I put the swollen hydrogels
into the drug solution. At different time intervals I take out some drug
solution, and dilute them to detect the concentration by HPLC. First I thought
this way is good to determine the drug uptaking kinetics. However the results
are not good because the concentration of the solution didn't change very
much.
Can you tell me how to determine the drug uptaking?
Thank you very much. |
p******e
发帖数: 164 | 2 why not put a dry one in the drug solution and let it swell and take drug?
thought
results
【在 w*****9 的大作中提到】
: Recently I am doing drug uptaking experiments. I put the swollen hydrogels
: into the drug solution. At different time intervals I take out some drug
: solution, and dilute them to detect the concentration by HPLC. First I thought
: this way is good to determine the drug uptaking kinetics. However the results
: are not good because the concentration of the solution didn't change very
: much.
: Can you tell me how to determine the drug uptaking?
: Thank you very much.
|
w*****9
发帖数: 14 | 3 I would like to do that too,because some peoples tell me that by this way more
drug will be uptaken.
Do you think both water and drug diffusing rates are same?
If not, water is faster, the concentration of surrounding solution will
increase. How can you get the uptaking kinetics by determing the concentration
of surrounding solution?
What kind of method I can use to determine amount of drug uptaking except to
release? |
d****i
发帖数: 360 | 4 What is the weight ratio of the drug solution to the swollen hydrogel? If this
ratio is too high, you should expect that drug drop in the drug solution
would be very small. How about use less drug solution but high drug
concentration? BTW, why do you want to do this kind of drug uptaking? at first
, I though it's about drug uptaking by cells!
thought
results
【在 w*****9 的大作中提到】
: Recently I am doing drug uptaking experiments. I put the swollen hydrogels
: into the drug solution. At different time intervals I take out some drug
: solution, and dilute them to detect the concentration by HPLC. First I thought
: this way is good to determine the drug uptaking kinetics. However the results
: are not good because the concentration of the solution didn't change very
: much.
: Can you tell me how to determine the drug uptaking?
: Thank you very much.
|
