e******t
发帖数: 3289 | 1 IF 3.4 分
Cell Physiol Biochem. 2013;31(1):113-22. doi: 10.1159/000343354. Epub 2013
Jan 25.
External Qi of Yan Xin Qigong inhibits activation of Akt, Erk1/2 and NF-&#
312;B and induces cell cycle arrest and apoptosis in colorectal cancer cells.
Yan X, Shen H, Jiang H, Hu D, Wang J, Wu X.
Source
Chongqing Institute of Traditional Chinese Medicine, Chongqing, China.
Abstract
Background/Aims: Colorectal cancer (CRC) is the second leading cause of
cancer death in the Western countries. Novel approaches of treatment are
needed for CRC. The purpose of the present study was to investigate
cytotoxic effect of external Qi of Yan Xin Qigong (YXQ-EQ) on human
colorectal cancer cells. Methods: The effect of YXQ-EQ on viability, cell
cycle progression and apoptosis in colorectal cancer HT-29 cells was
investigated. Phosphorylation of Akt and Erk1/2, activation of NF-ĸB
and the expression of proteins involved in regulation of cell cycle and
apoptosis were examined by Western blot analysis. Results: YXQ-EQ markedly
decreased viability and blocked colony formation of HT-29 cells. YXQ-EQ
downregulated cyclin D1 expression and increased accumulation of cyclin-
dependent kinase inhibitors p21(Cip1) and p27(Kip1), resulting in G1 cell
cycle arrest. YXQ-EQ induced apoptosis in HT-29 cells in association with
decreased expression of antiapoptotic proteins Bcl-xL, XIAP, survivin and
Mcl-1 and elevated expression of proapoptotic protein Bax. YXQ-EQ
significantly repressed phosphorylation of Akt and Erk1/2 and NF-ĸB
activation in HT-29 cells, suggesting that YXQ-EQ may exert cytotoxic effect
through regulating signaling pathways critical for cell proliferation and
survival. Furthermore, YXQ-EQ treated PBS and an YXQ-EQ treated plant
extract induced apoptosis in HT-29 cells. Conclusion: These findings show
that YXQ-EQ has potent cytotoxic effect on HT-29 cells and suggest that YXQ-
EQ could be potentially used for colorectal cancer treatment either directly
or indirectly via carriers.
Copyright © 2013 S. Karger AG, Basel. | M*****n
发帖数: 16729 | 2 俺们以前实验室也做过一个气功的实验。
好像是97，98年的时候，气功可以提高ecoli的转化效率。
cells.
【在 e******t 的大作中提到】
: IF 3.4 分
: Cell Physiol Biochem. 2013;31(1):113-22. doi: 10.1159/000343354. Epub 2013
: Jan 25.
: External Qi of Yan Xin Qigong inhibits activation of Akt, Erk1/2 and NF-&#
: 312;B and induces cell cycle arrest and apoptosis in colorectal cancer cells.
: Yan X, Shen H, Jiang H, Hu D, Wang J, Wu X.
: Source
: Chongqing Institute of Traditional Chinese Medicine, Chongqing, China.
: Abstract
: Background/Aims: Colorectal cancer (CRC) is the second leading cause of
| c**********u
发帖数: 7276 | | w********9
发帖数: 8613 | 4
有可重复性吗？
【在 M*****n 的大作中提到】
: 俺们以前实验室也做过一个气功的实验。
: 好像是97，98年的时候，气功可以提高ecoli的转化效率。
:
: cells.
| l*********1
发帖数: 772 | 5 这个是什么mechanism? 有解释是怎么提高的吗？
【在 M*****n 的大作中提到】
: 俺们以前实验室也做过一个气功的实验。
: 好像是97，98年的时候，气功可以提高ecoli的转化效率。
:
: cells.
| M*****n
发帖数: 16729 | 6 heat-shock
不过俺不知道是不是发paper了
上海有个气功研究所，他们跟俺们collaborate的。
【在 l*********1 的大作中提到】
: 这个是什么mechanism? 有解释是怎么提高的吗？
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