h******y
发帖数: 351 | 1 请问做ADME/Tox的同仁，有没有好的in vitro细胞模型具有高表达的OAT1, OAT3, OCT2
等肾脏特异的Transporter? | b****y
发帖数: 267 | 2 HEK cells. Most labs and companies have those over expressed cell lines.
请问做ADME/Tox的同仁，有没有好的in vitro细胞模型具有高表达的OAT1, OAT3, OCT2
等肾脏特异的Transporter?
★ Sent from iPhone App: iReader Mitbbs 7.52 - iPad Lite
【在 h******y 的大作中提到】
: 请问做ADME/Tox的同仁，有没有好的in vitro细胞模型具有高表达的OAT1, OAT3, OCT2
: 等肾脏特异的Transporter?
| h******y
发帖数: 351 | 3 Although the HEK/OATs over-expressing cells can be used to study drug uptake
, they do not form epithelial barrier on Transwell, so it is impossible to
study drug efflux using this model. Plus, HEKs lack other important
membrane transporters besides OATs/OCTs.
What do you think about primary RPTECs? Do they suffice for in vitro cell
model?
OCT2
【在 b****y 的大作中提到】
: HEK cells. Most labs and companies have those over expressed cell lines.
:
: 请问做ADME/Tox的同仁，有没有好的in vitro细胞模型具有高表达的OAT1, OAT3, OCT2
: 等肾脏特异的Transporter?
: ★ Sent from iPhone App: iReader Mitbbs 7.52 - iPad Lite
| b****y
发帖数: 267 | 4 So far, not a good model is available for this purpose.
Although the HEK/OATs over-expressing cells can be used to study drug uptake
, they do no........
★ Sent from iPhone App: iReader Mitbbs 7.52 - iPad Lite
【在 h******y 的大作中提到】
: Although the HEK/OATs over-expressing cells can be used to study drug uptake
: , they do not form epithelial barrier on Transwell, so it is impossible to
: study drug efflux using this model. Plus, HEKs lack other important
: membrane transporters besides OATs/OCTs.
: What do you think about primary RPTECs? Do they suffice for in vitro cell
: model?
:
: OCT2
|
|
