m****g
发帖数: 530 | 1 mRNA的末端区域通常被认为是无足轻重的，但是有研究表明，这个区域实际上可能承担
着预防正常细胞转变成癌细胞的重要责任。这项研究结果发布在8月20日的《cell》上。
蛋白质是由细胞DNA的模板生成的。这种模板叫信使RNAs（messenger RNAs，mRNAs），
它包含三个区域。中间区域是编码蛋白的，起始端和末端是非翻译区（UTRs），因为它
们不能编码蛋白的任何部分。起始端能够促使蛋白质翻译开始，而尾部，即3'UTR，似
乎只是简单的跟随而已。所以mRNA的末端经常被认为是不重要的。
文章的第一作者Christine Mayr解释说，目前我们已经证实通常mRNA末端有一个蛋白质
翻译调控程序，而且在某些情况下对癌症的发生具有重要作用。
几乎所有的生物过程细胞都需要利用蛋白质，从细胞分裂，到转运一些机体必不可少的
分子，到维持细胞结构等等。由于细胞的蛋白产生是受严格调控的，不同的细胞类型产
生的蛋白也是不一样的。如果在细胞的生命周期中蛋白的供应过量或不足就会改变细胞
正常的功能。而这些改变可以导致细胞无限生长和转移，而这两者正符合了癌细胞的特
征定义。
通过比较正常细胞和癌细胞 |
a****o
发帖数: 1786 | 2 虽然第一段话很好笑，但是文中发现还是很有意思的
上。
【在 m****g 的大作中提到】
: mRNA的末端区域通常被认为是无足轻重的，但是有研究表明，这个区域实际上可能承担
: 着预防正常细胞转变成癌细胞的重要责任。这项研究结果发布在8月20日的《cell》上。
: 蛋白质是由细胞DNA的模板生成的。这种模板叫信使RNAs（messenger RNAs，mRNAs），
: 它包含三个区域。中间区域是编码蛋白的，起始端和末端是非翻译区（UTRs），因为它
: 们不能编码蛋白的任何部分。起始端能够促使蛋白质翻译开始，而尾部，即3'UTR，似
: 乎只是简单的跟随而已。所以mRNA的末端经常被认为是不重要的。
: 文章的第一作者Christine Mayr解释说，目前我们已经证实通常mRNA末端有一个蛋白质
: 翻译调控程序，而且在某些情况下对癌症的发生具有重要作用。
: 几乎所有的生物过程细胞都需要利用蛋白质，从细胞分裂，到转运一些机体必不可少的
: 分子，到维持细胞结构等等。由于细胞的蛋白产生是受严格调控的，不同的细胞类型产
|
n********k
发帖数: 2818 | 3 in fact, it is ridiculous:))...anything novel insight or discovery except
the big name? genome scale???
【在 a****o 的大作中提到】
: 虽然第一段话很好笑，但是文中发现还是很有意思的
:
: 上。
|
a****o
发帖数: 1786 | 4 I guess the prevalence of shorter 3' UTR in cancer cells is something new;
shortening 3' UTR of a particular gene can lead to turmor formation is
another selling point.
I only read the abstrct and not very familar with cancer biology, so bricks
please.
【在 n********k 的大作中提到】
: in fact, it is ridiculous:))...anything novel insight or discovery except
: the big name? genome scale???
|
n********k
发帖数: 2818 | 5 has not read the paper..but as far as my memory goes,(I could be wrong
though), the very importance of
3UTR in gene expression regulation has been demonstrated numerous times in
numerous conditions I believe,
pretty much textbook knowledge now, as for cancer, don't know but would be
highly predicated...maybe the
prevalence/genome scale is a selling point, a particular gene, which gene
and I highly suspect it would be a
first report for such secenario as 3-UTR's importance has been recognized
for
【在 a****o 的大作中提到】
: I guess the prevalence of shorter 3' UTR in cancer cells is something new;
: shortening 3' UTR of a particular gene can lead to turmor formation is
: another selling point.
: I only read the abstrct and not very familar with cancer biology, so bricks
: please.
|
n********k
发帖数: 2818 | 6
bricks
【在 a****o 的大作中提到】
: I guess the prevalence of shorter 3' UTR in cancer cells is something new;
: shortening 3' UTR of a particular gene can lead to turmor formation is
: another selling point.
: I only read the abstrct and not very familar with cancer biology, so bricks
: please.
|
n********k
发帖数: 2818 | 7 Just scanned through the paper in about 10 mins, so u know what I mean...
anyway. I am not impressed at all,
kind of everything is known before but they put it altogether. I am rather
surprised the reviewers let it go...the
cherry pick nature of candidate genes is so obvious...I wouldn't buy that
it was not cherry-picked...what I
would get from the paper is alternative splicing variants matters and MiRNA
regulation matters etc...maybe
someone could tell me whether the demonstration of short iso
【在 a****o 的大作中提到】
: I guess the prevalence of shorter 3' UTR in cancer cells is something new;
: shortening 3' UTR of a particular gene can lead to turmor formation is
: another selling point.
: I only read the abstrct and not very familar with cancer biology, so bricks
: please.
|
n********k
发帖数: 2818 | 8 And I bet that people could come out with a list of genes cherry picked and
then reach exactly the opposite
conclusion---the prevalence of longer 3-UTR in cancer line...
MiRNA
【在 n********k 的大作中提到】
: Just scanned through the paper in about 10 mins, so u know what I mean...
: anyway. I am not impressed at all,
: kind of everything is known before but they put it altogether. I am rather
: surprised the reviewers let it go...the
: cherry pick nature of candidate genes is so obvious...I wouldn't buy that
: it was not cherry-picked...what I
: would get from the paper is alternative splicing variants matters and MiRNA
: regulation matters etc...maybe
: someone could tell me whether the demonstration of short iso
|
n********k
发帖数: 2818 | 9 I couldn't believe that the reviewers would let it go without asking them to
do the genome scale analysis for
such a conclusion...
and
【在 n********k 的大作中提到】
: And I bet that people could come out with a list of genes cherry picked and
: then reach exactly the opposite
: conclusion---the prevalence of longer 3-UTR in cancer line...
:
: MiRNA
|
a****o
发帖数: 1786 | 10 totally agree with you. I thought they mapped 3' end of RNA by deep
sequencing and found in cancer cells, 3'UTR of a large number of genes are
shorter than their counterparts in normal cells. It seems the prevelance I
mentioned is only 16 genes, although at a very high ratio, 16/23.
to
【在 n********k 的大作中提到】
: I couldn't believe that the reviewers would let it go without asking them to
: do the genome scale analysis for
: such a conclusion...
:
: and
|
n********k
发帖数: 2818 | 11 if u like, u could transfer the discussion on iPS to this club and see
anyone here likes to have a more indepth discussion on it...
bricks
【在 a****o 的大作中提到】
: I guess the prevalence of shorter 3' UTR in cancer cells is something new;
: shortening 3' UTR of a particular gene can lead to turmor formation is
: another selling point.
: I only read the abstrct and not very familar with cancer biology, so bricks
: please.
|
a***e
发帖数: 1010 | 12 besides luciferase assay, they even don't bother to do a western blot to
check endogenous protein expression level. Many of his candidates have
antibodies available.
anyway, it is Bartel.
Another problem is that the authors didn't answer whether these UTR
shortening is the cause or the result of tumor biogenesis. Although they
try to get microRNA into the story, the effect on translation is so weak.
However, one wield thing to me is that why their short formed mRNA is more
stable than long form |
n********k
发帖数: 2818 | 13 Gosh, you really have time:)))....obviously you read it carefully....I spent
about 10 mins on it and already feeling
I am not wasting my time:))), seriously, it is very much a crap study with
big name and catching theme but has
tons of wholes...I would tend to believe that they had the story in mind
before she even started the work....But
it doesn't seem it damage her career with such sloppy study as she seems in
SKCC now....
am
【在 a***e 的大作中提到】
: besides luciferase assay, they even don't bother to do a western blot to
: check endogenous protein expression level. Many of his candidates have
: antibodies available.
: anyway, it is Bartel.
: Another problem is that the authors didn't answer whether these UTR
: shortening is the cause or the result of tumor biogenesis. Although they
: try to get microRNA into the story, the effect on translation is so weak.
: However, one wield thing to me is that why their short formed mRNA is more
: stable than long form
|
