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全部话题 - 话题: plate
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Y***r
发帖数: 15270
1
来自主题: Travel版 - 迈阿密Toll by plate的问题
租车公司会帮你交的,然后寄账单给你,有的会加收10块钱手续费。
[在 lostpapaya (lostpapaya) 的大作中提到:]
:上个星期去佛罗里达玩,在奥兰多的机场租了辆车,然后开到迈阿密。
:奥兰多附近的Toll Road有可以投币Lane, 就都投币了,但是到了迈阿密的时候变成了
:Sun Pass or Toll By Plate,都没有可以投币的地方。
:现在租的车已经还掉了,但是不太清楚这种Toll By Plate应该怎么办?我应该去哪里
:交钱?
:谢谢!
t*******i
发帖数: 4960
2
plated的比filled的便宜。
filled我觉得是按一定比例参合在一起,plated就是表面一层。
s*******1
发帖数: 2366
3

filled你的理解是个误区... filled也是表面一层,只是比plated厚。
filled是用金箔辗在base metal上,plated是用溶液做类似电镀那种。
filled印象中要求金层的重量要占到总重量1/20以上才允许叫gold filled.
“按一定比例参合在一起”的是K金。
s*******1
发帖数: 2366
4

同样重量14k贵得要多得多得多得多……
拿火山上的价格举个例子:
同样款式基本同样大小的这两个clasp,镀金的不到$0.25一个,14k的将近$70。
http://www.firemountaingems.com/details.asp?PN=H206165FY
http://www.firemountaingems.com/details.asp?PN=H201036FS
gold-filled常有12k和14k的金层。但是plated的极少会说是多少k的镀层,因为本身就
没大意义。偶甚至觉得给plated加上多少k在前面,基本就是忽悠搞不清楚材料的人的.
..
戴的话看rp了,一次掉色的也有,几次掉色的也有,仔细点的话偶觉得也就几个月吧。
z****6
发帖数: 10776
5
来自主题: PhotoForum版 - Ian Ruhter: Holga + wet plate (zz)
http://www.petapixel.com/2012/12/18/how-to-use-a-holga-as-a-han
How to Use a Holga as a Handheld Wet Plate Camera
Wet plate photographer Ian Ruhter has received a good deal of attention over
the past year for using a custom camera van to create giant collodion
process metal photos. When he’s not turning large sheets of metal into
photographs, he’s sometimes working on the opposite side of the spectrum.
a***a
发帖数: 40617
6
来自主题: PhotoGear版 - RRS plate and Lee filter system minireview
大图请看
http://www.pbase.com/anoia/for_pentax_67ii
RRS B68 plate for pentax 67II
Lee speical 100mm push-on filter holder system
RRS的板子最大的问题如图所示,板子的底部太厚,导致卡在arca-style的clamp上时
无法严丝合缝,有很大空袭,也增加了同一个clmap跟不同plate之间通用性匹配的难度。
好像这是RRS板子的普遍问题。
Lee speical 100mm push-on filter holder system.是专门为几个中大幅面的95mm口径
广角头设计。最大的好处就是不会有暗角和遮挡的问题。无须任何adpater ring
原来设计的目的是直接套在镜筒上。然后插filter,再在最外面加一个cpl
但是因为cpl会在最外侧,所以口径加大为105mm才能消除暗角,好处是方便调整cpl
但是105mm口径无论lpl还是cpl都价格不菲,并且这个口径的PL都没有coating,所以
我考虑是否可以用价格差不多的95mm口径有coating的CP
x**0
发帖数: 6149
7
来自主题: PhotoGear版 - [FS] RRS L-plate BD80-L
器材名称
RRS L-plate BD80-L
付款方式(需要其他付款方式请联系卖家)
personal paypal, BoA, cash...
卖家联系方式
bbs pm
器材具体描述、价格、运费:
(均为现金/BOA/check/MO或non-cc paypal价,+$5 for echeck)
(其他付款方式引起的价格变动请与卖家联系)
Really Right Stuff L-plate BD80-L,可用于D80,可能也能用于D90(没验证过),
有正常使用的磨损痕迹,没有功能缺陷,$80 including shipping
a*****0
发帖数: 3319
8
I bought a Markins Q3-TrQ Black.I checked Markins web site, and saw many
kinds of plates. I am at a loss which one to buy.
I have a canon 60D camera, and a canon VIXIA HV30 camcorder. I'll purchase a
new camcorder soon. And in the future, I am planning to buy a Canon Mark
5DII. I hope the plate can work on these camera and camcorder.
Any recommendation or help would be greatly appreciated.
a*****0
发帖数: 3319
9
I bought a Markins Q3-TrQ Black.I checked Markins web site, and saw many
kinds of plates. I am at a loss which one to buy.
I have a canon 60D camera, and a canon VIXIA HV30 camcorder. I'll purchase a
new camcorder soon. And in the future, I am planning to buy a Canon Mark
5DII. I hope the plate can work on these camera and camcorder.
Any recommendation or help would be greatly appreciated.
x5
发帖数: 27871
10
新的也就50~60一块,贵的是L-plate和replacement foot
加起来不过500的样子,而且还可以买二手的
再说了,你的长板都是给炮的吧,快装版一般人弄个两三块足够了
a*****0
发帖数: 3319
11
谢谢。我已经买了Markins Q3T with lever clamp了。我后来也发现knob release has
the versatile advantage of matching different brands of plates.
x5
发帖数: 27871
12
Markins的level clamp设计的不错,可以换左右手,可以调节松紧,我就用的这个
你plate用的不多的话,盯着一家的买就可以了
糖爸的意思是用screw release话可以用各家的牌子,不过我还是觉得level的方便一些

has
h*******e
发帖数: 8370
13
哈哈哈哈
一年半以前我给国内带的那支545。mint condition。
comes with
真货lens coat一套
CPL
居然还带一个kirk compatible plate,山寨货但是是白的(!!!),跟镜头一个颜
色。
我找找,说不定能问到这个plate是哪弄的。挖哈哈哈
这东西价格我是记得的,30块。
D***3
发帖数: 12712
14
来自主题: PhotoGear版 - 求, used RRS L-plate for 6D
RRS L-plate 与其他L-plate 有啥不同?
o**s
发帖数: 3412
15
来自主题: PhotoGear版 - 求, used RRS L-plate for 6D
鎴戞湁涓猤eneric 鐨勶紝涓嶆槸涓撻棬涓鐨勶紝 绔栨媿鐨勬椂鍊欏鏄撳姩銆傛兂
涔颁釜6d specific 鐨勩鍝釜鐗屽瓙鏈ソ閮芥槸涓汉鍠滃ソ浜嗐
銆鍦DRGX3(椋濔獔 鐨勫ぇ浣滀腑鎻愬埌: 銆br />

: RRS L-plate 涓庡叾浠朙-
plate 鏈夊暐涓嶅悓锛br />

D***3
发帖数: 12712
16
来自主题: PhotoGear版 - 求, used RRS L-plate for 6D
你的意思用其他牌子L-plate 竖的时候锁不紧机身
用RRS L-plate就没这问题?
quick release上面好像有螺丝可以控制松紧......
x*****o
发帖数: 441
17
来自主题: Biology版 - 关于做agar plate的问题
新手,有点问题请教大家.
做agar plate,没有那种灭菌工作台,就是在自己的bench上,点一个煤气灯. LB agar倒
上去后,盖上盖子,盖子一会就有很多蒸汽形成的水.我看有人是在灭菌工作台里面,开盖
子晾一会.在自己的bench上就不可能了.这个大家是怎么解决的?
还有就是含有抗生素的plate,加抗生素时候溶液不能太烫,不过也不能太凉,是不是还有
点烫手的时候加?大家是怎么掌握的?
问了很多问题. :-)
j****p
发帖数: 16
18
来自主题: Biology版 - 关于做agar plate的问题
可以自己做一个盒子,在盒子里面开盖放plate. 最简单的就是用一个装1mL pipet tip
的盒子或者盒盖倒扣在plate上。
y**i
发帖数: 1050
19
谁要低吸附Ultra-low attachment 96 plate
各位同行大家好,本人现在有两箱 Ultra-low attachment(低吸附)的96孔板,
Corning的,未
拆封,完全新的,由于实验室订的多了,现在由我来出售,具体型号如下,
Costar 96 well plate with lid, flat bottom, ultra-low attachment surface
sterile, polystyrene,
1/PACK, 23/CASE, Lot No: 24210010
Corning No: 3474
Corning Incorporated
Made in USA
原价:Case of 24 for $267.80,现以便宜出售,
该板,我们实验室用来养tumorsphere,干细胞球等,所以如果哪位同行对该板有兴趣,
可以与我联
系,总共两箱,先到先得。可以通过EMAIL与我联系 我会及时和你联系。该板我会邮寄
给您。绝对低于
市场价格。
谢谢
T**********t
发帖数: 1604
20
That's two questions indeed.
That no colonies grew might result from many things. Not the right
antibiotic or not the right amount, or transformation went wrong... You need
to include at least a positive control and negative control each time when
you do transformation in order to troubleshoot.
Air bubbles in the plate usually happen when the media was too cold when
pouring the plates. The LB-Agar solidified before the accidentally trapped
air bubble had the chance to escape. The viscosity of th... 阅读全帖
s**o
发帖数: 114
21
一个国内朋友之前通过在美华人中间商做tissue culture plate, 后来那个美国华人自
己开厂了,不带他玩了。自己有一条生产线,空在那里,不知道产品是做什么的,该卖
给谁。呵呵。
请各位专家说说,tissue culture plate哪里最需要啊?卖给谁啊?
A******y
发帖数: 2041
22
MD's plate reader are about twice as expensive then their competitors and do
about half the work. Tecan, Biotek, and BMGlabtech plate readers are all
better than MDs. I only used Tecan one but both Bioteck and BMGlabtech
based on their specs offer more function such as alpha scan. Tecan's liquid
handling is better though, if you are actually buying the liquid handling
portion.
s********n
发帖数: 2939
23
这边有Tecan, MD和Beckman的,我最常用的是MD的,觉得很不错,尤其是能够实时监测
96-well plate,测生长曲线很有用。Tecan可以加一个staker,能够自动load几十个
plate,也很不错。Beckman的也不错,不过得换不同的filter比较麻烦,不过其biomek
FXp系统就很好用。
p*****m
发帖数: 7030
24
哪里有卖的?找了几家看到都是要用配套的96 well plates,不是能给任意的plate用的
谢谢!
s******y
发帖数: 28562
25
这个完全取决于你们的plate reader 是怎么装备的,只要里面装有荧光
激发装置,荧光检测仪,以及合适的滤镜,随便什么荧光蛋白都可以检测。
但是,大部分plate reader,都没有装置这种设备,要么就是读透光度,要么就是
读自发荧光,这些装备是不能用来看激发性荧光的。
f*********8
发帖数: 129
26
夫妻店,真是不好呆。
在做bacterial artificial chromosome constructs,实验室没人做过,大家都比较紧
张。我在筛clone,按照别人给的protocol,clone 长出来后,先挑在100微升的LB,长一
会,再分开几部分作functional test, pcr,之类的筛选。因为medium有tetracycline,
要避光,我就把含有colony的eppen tube放在盒子里,shaking,这样既避光,也方便。
然后,下午老板问我了这个问题,他说,老板娘告诉他,somebody is shaking plates
(其实他们知道是我,因为用的是30度 shaker,就我在用,他们有时要避光的plates,也是放在盒子里,在放在这个shaker,因为没有另外的30度incubator)
这样的常识我还是知道的。就算一时糊涂,也是浪费一些电而已。在这个实验室,我已
经是小心小心再小心了。知道老板娘,什么都看在眼里。可是你别看错啊,还马上打报告。真郁闷。
f*********8
发帖数: 129
27
我在摇tube,老板娘以为我在摇plates (长E. coli的),老板娘就跟老板报告了,老板
就问我是不是在 shaking plates
s******y
发帖数: 28562
28
可能是因为你是新手,他们不放心你的实验技能吧。
应该不是因为省电的问题,实验室再省也不会不开shaker, 那点电钱相对于
实验室材料的钱和你的工资来说简直就什么都不是。
我猜他们应该是担心你不懂干活乱做实验,把agar plate (?) 放进去摇。
稍微解释一下就好了。等你一些初步结果出来之后,他们知道你是能干活的人,
一般来说应该就会好一些。

tetracycline,
plates
,也是放在盒子里,在放在这个shaker,因为没有另外的30度incubator)
报告。真郁闷。
f*********8
发帖数: 129
29
我今天又摇,她竟然把shaker停掉了,也不跟我说一下,太让人生气了。我明天还得摇
,我得找机会给她看看,我里面摇得是eppen tube,是管子,不是agar plates. 我昨天
跟老板说了,难道她还以为我撒谎?真是令人发指。

tetracycline,
plates
,也是放在盒子里,在放在这个shaker,因为没有另外的30度incubator)
报告。真郁闷。
h**********r
发帖数: 671
30
我圡,没怎么用过microtiter plate reader,都是用分光光度计来着,但是太费比色
皿。准备去别的实验室蹭microtiter plate reader,问题是OD超过1或者2是不是就要
稀释呢?问过他们实验室的人,也不清楚。请教了!
另外用nanodrop测DNA呢?上次测了个500ng/ul,应该也就不准了吧?
多谢!
i***0
发帖数: 160
31
To be honest, according to my experiences, the worst reliability of a plate
reader is the absorption assays. I have tried Bradford or OD280, both give
high standard deviation. However, if you just want a guess, try using your
sample at original conc, 1:1 dilution, and 1:4 dilution in triplicate. You'd
better have BSA standards at least 5 concentrations in the same plate in
triplicate. These will give you a relatively reliable readout. OD is linear
at 0.5 to 1, roughly. good luck
a***y
发帖数: 19743
32
来自主题: Biology版 - 求推荐倒好的LB plates
求推荐价格便宜量又足的倒好的LB plates
看到Fisher上有MP Biomedicals LB Agar Medium Plates
100个$194,但是不知道好不好。
如果哪位有用的满意的请推荐一下吧。
现在从core哪里拿是$3一个,但是因为包装方式很容易干。
谢谢!
T****o
发帖数: 716
33
早上好!
问题是这样:
在倾斜混凝土面上立wide flange column,一个选择是浇个小的混凝土Pad,得到水平
面,然后放柱、base plate,浇grout;另一个说法是,不浇混凝土pad,直接浇grout
pad就行了,5 Star Non-thrink Grout。
问问大家,不用混凝土Pad可以吗??在坡度上base plate以下需要厚度约为 1-6
inches。
多谢!!!
n******n
发帖数: 1030
34
可不可以cut WF beam and weld it to base plate?
这个不是常规做法,可能要多算算PLATE bending, shear, block shear以及bolt
tension & shear.

grout
T****o
发帖数: 716
35
柱子可以和base plate焊接好,shop weld & field erection,问题是如果要erector
来处理任何倾斜面,总是难以让人放心,所以一定要柱子和plate垂直,现场做bolted
connection。
说道仔细的计算,别人都嫌麻烦呢。。。
l*****o
发帖数: 82
36
来自主题: Macromolecules版 - any guy know some about plating?
Electro-plating knowledgy can eazily be found in those MEMS books.
Also, in X-RAY lithography, electro-plating of Ni or Au is a standard
method for mask fabrication.
As for those photoresist, you can get rid of them using O2 plasma etching.
You can go to www.nnun.org to looking for some parameters. (From this addr,
go stanford nano center or Cornell nano center. They have basic lithograph
knowledge and receipe)
c*****e
发帖数: 44
37
How to calibrate the phase retardance of a wave-plate quantitively?
I worried the quantity of the thorlabs' quater-wave plate I have, it seems
some anti-reflection coating is already gone.
z*******n
发帖数: 1034
38
January 24, 2015 9:07 AM
Daniel Terdiman
Updated at 12:46pm Pacific with a statement from the DMV.
In an abrupt turn of events, the California Department of Motor Vehicles has
retracted a new rule that forced drivers of services like Uber and Lyft to
get commercial plates for their vehicles.
Earlier this month, the DMV issued the ruling, which seemed to contradict a
California law that said that drivers for such services were people using
their “personal vehicles.” The DMV’s new edict also count... 阅读全帖
c******n
发帖数: 4965
39
来自主题: _Auto_Fans版 - skid plate and oil change.
when changing oil filter. u have to take off the skid plate right?
mine got just a plastic cover that's like a skid plate
L*****g
发帖数: 311
40
来自主题: _Auto_Fans版 - F80 M3 camber plate/negative camber
两次半track day,大概一共有5小时在赛道上跑,front tire的外侧已经
快磨没了。今天去做BMW club的tech inspection,被告知得换前轮胎了。
换轮胎事小,但要是过一个月新轮胎又磨没了,那就太花钱了。跟technician讨论了一
下解决方案,似乎只能换第三方camber plate(stock plate的调教范围太小,没法帮
助),调negative camber(他说start with 2.5degree)。材料加人工一共要1,900。
还有technician建议换Hawk的brake pad,不如Pagit RS29那么hardcore。
大家看看合理不?hangjin,你的车轮胎没有这个问题么?
v******d
发帖数: 227
41
来自主题: _Auto_Fans版 - F80 M3 camber plate/negative camber
所有McPherson前悬挂的车都有开的猛外侧轮胎磨损过快的问题,把前camber调整到-2.
5是个正确的解决方案. 但camber plate 不需要这么多钱, plate本身只有600,安装费
400,加上alignment 100,撑死1.1k.

900。
L*****g
发帖数: 311
42
来自主题: _Auto_Fans版 - F80 M3 camber plate/negative camber
camber plate 6-7 hours的labor,这个店似乎是我们这儿比较有名的改车店的,还赞
助过一些比赛。
Valeyard似乎是西雅图地区的,你知道car tender算有名的改车店么?
问了另外一个店,labor quote是900 - 1k,而且以前还没做过,不知道什么camber
plate能用在F80 stock悬挂上面。这个就更不敢用了。
w*******y
发帖数: 60932
w*******y
发帖数: 60932
44
I've been looking to buy the Porter Cable 7424XP dual action polisher for
many weeks, hoping to find a better deal than the $125 for polisher +
backing plate only at toolsplus. Well autogeek has a better deal, for only a
few hours today. You can get the polisher plus a whole load of goodies (
worth maybe $100) for $149.99. The ad suggests the "up2" code to upgrade
shipping to 2nd day, but unless you are in a hurry, use code "ago" to get
free shipping (saves you $20 or more).
link to sale:
http... 阅读全帖
w*******y
发帖数: 60932
45
It says "Dessert Size" Plates ..not sure what the exact size is
20-piece Set (in red or pink)
$4.94 when you enter code RDSUNDAY45
Free shipping
Set includes 4 each:
Dessert size plates
Teaspoons
Dessert spoons
Knives
Forks
Flatwear tops are stainless w/ strudy melamine handles
w*******y
发帖数: 60932
46
3 Pack 6 Ft Gold-Plated Super High Resolution HDMI M/M Cable Version 1.4 -
NEW - Supports 4096 x 2160p - Non-Ethernet:
http://www.buy.com/prod/3pak-6-ft-gold-plated-super-high-resolu
Marketing Description Please note this cable doesn't support HDMI
Ethernet Channels, but it supports ALL other HDMI 1.4 specifications.
Brand NEW HDMI Standard version 1.4 for your HDTV or 3DTV. Please note this
cable doesnt support HDMI Ethernet Channels, but it supports all other HDMI
1.4 specifications. If yo... 阅读全帖
w*******y
发帖数: 60932
47
2-Pack 6 Ft Gold-Plated Super High Resolution HDMI M/M Cable Version 1.4 -
Non-Ethernet - Supports 4096 x 2160p:
http://www.buy.com/prod/2-pack-6-ft-gold-plated-super-high-reso
Features
Video Features 3D Over HDMI Defines input/output protocols for major 3D
video formats, paving the way for true 3D gaming and 3D home theater
applications.
Video Features 4K x 2K Video Support Enables video resolutions far
beyond 1080p, supporting next-generation displays that will rival the
Digital Cinema... 阅读全帖
w*******y
发帖数: 60932
48
Bonton currently has some excellent deals in fiestaware! Everything seems
to be 50% off.
Free shipping with code 2011SELECTGUEST
Bonton's Fiestaware:
http://www.bonton.com/shop/home/dining-entertaining/casual-dinn
They even have the hard to find smiley face luncheon plate!!
Smiley face plate:
http://www.bonton.com/shop/home/fiesta-dinnerware-smiley-lunche
and a set of two bowls even in the new marigold
Bonton's Fiestaware:
http://www.bonton.com/shop/home/dining-entertaining/casual-dinn
Happy sh... 阅读全帖
w*******y
发帖数: 60932
49
Free shipping over $50 or just order 21 cartons (21,000 paper plates)
Each carton of 1000 plates is only $2.49. Looks like a price mistake.
Similar quantities are $25+ at other websites/stores
http://www.officemax.com/catalog/sku.jsp?productId=AR S24817
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