m**********3
发帖数: 706 | 1 I am doing a coIP (use a mouse antibody to pull down and use a rabbit
antibody for IB) and found my band
overlap with a rabbit IgG band. Any reccomendation for a good secondary
without IgG band or coIP? |
s******y
发帖数: 28562 | 2 I am a little bit confused.
Why don't you use protein A/G to do the pulldown?
Why do you want to use 2nd ab for pulldown?
【在 m**********3 的大作中提到】
: I am doing a coIP (use a mouse antibody to pull down and use a rabbit
: antibody for IB) and found my band
: overlap with a rabbit IgG band. Any reccomendation for a good secondary
: without IgG band or coIP?
|
d****u
发帖数: 1553 | |
n******d
发帖数: 1055 | 4 stop doing IP. that is the solution. |
s***a
发帖数: 671 | 5 如果你说的是heavy chain band 干扰的话，可以尝试light chain specific的二抗。 |
p******i
发帖数: 1092 | 6 同問
【在 d****u 的大作中提到】
: 你的膜上为什么会有rabbit IgG？
|
