由买买提看人间百态

topics

全部话题 - 话题: marker
1 2 3 4 5 6 7 8 9 10 下页 末页 (共10页)
w*******y
发帖数: 60932
1
OfficeMax.com 100% back in rewards on select markers, pens, and compass 12/
01-12/03
This is still a work in progress as OfficeMax has not updated the MaxPerks
Rewards page which will include the terms.
The product pages have been updated though ( to MinhTrinh).
MaxPerks Rewards offers for the week: LINK. Log into your rewards account
to view ad until OfficeMax.com MaxPerks page is updated for this week.
December 01, 2011 thru December 03, 2011: Link to all 100% back in rewards
items
Sharpie Tw... 阅读全帖
w*******y
发帖数: 60932
2
OfficeMax.com 100% back in rewards on select markers, binder clips, pens,
and Expo items 11/24-11/26
MaxPerks Rewards offers for the week: LINK. Log into your rewards account
to view ad until OfficeMax.com MaxPerks page is updated for this week.
This week OfficeMax is offering two separate MaxPerks rewards offers. One
runs from 11/20-11/23 and the other from 11/24-11/26. For the offer
starting 11/20, please see the thread:
OfficeMax.com 100% back in rewards on select markers, highlighters and... 阅读全帖
w*******y
发帖数: 60932
3
OfficeMax.com 100% back in rewards on select markers, highlighters and
liquid pencils 11/20-11/23
MaxPerks Rewards offers for the week: LINK. Log into your rewards account
to view ad until OfficeMax.com MaxPerks page is updated for this week.
This week MaxPerks is offering two separate MaxPerks rewards offers. One
runs from 11/20-11/23 and the other from 11/24-11/26. For the offer
starting 11/24, please see the thread:
OfficeMax.com 100% back in rewards on select markers, binder clips, pens,
... 阅读全帖
w*******y
发帖数: 60932
4
Crayola Color Wonder Magic Light-Up Markers
Free ship to store from Walmart
Walmart:
http://www.walmart.com/ip/Color-Wonder-Magic-Light-Up-Markers/1
Create anytime and anywhere with Color Wonder Magic Light Up Markers.
Crayola Color Wonder Markers magically recognize and light up each of the
marker colors! Simply click the marker into the light wand and the barrels
light up to identify each marker color. Includes 6 color wonder markers, one
tethered magic light wand and 24 pages of color wonder ... 阅读全帖
a******g
发帖数: 72
5
marker_X220_T420_etc.7z
http://www.multiupload.com/UL60PV777D
dos.img镜像用ultraISO写入USB闪存盘,即制作好了启动盘。
把marker目录及文件copy到制作好了的U盘启动盘上。
设置bios, 用U盘启动.
◆DOS下 进入marker目录
CD marker
◆①命令bak.bat备份BIOS到BIOS.ROM文件
bak.bat
◆②[然后,用16进制编辑器查找 E3EDC4000100010003000000 ,如图修改BIOS, 只需
改2个字节就可以了]
注:
现在Thinkpad的任何BIOS其实都是含有SLP 2.1信息的。(有SLIC 2.1表的,就不多说
了。不需要任何操作,直接装OEM win7就是了。)
DOS机没有SLIC 2.1表的是这次的修改对象。
大家备份一下Bios, 得到BIOS.ROM. 用16进制编辑器(例如Windows下UltraEdit)打开
BIOS.ROM,查找十六进制数据块十六进制E3EDC4000100010003000000定位。
SLP块数据一般都是8... 阅读全帖
z****u
发帖数: 1007
6
来自主题: Biology版 - 关于stem cell marker的筛选的设想
几年前基于inducible Cre的lineage tracing技术的出现对stem cell的研究是关键的
。但并没有很多stem cell 的 in vivo marker被identified出来。比如说liver
tissue,都知道其可以再生,但是它有没有stem cell仍然在争议。更莫提marker了。
endothelium是个快速turn
over的组织,但起在成体内stem cell population的identification仍然未知。
筛选stem cell marker的方式可以有两个途径
第一就是正向的从已知marker出发,比如说通过已知marker筛选出某stem cell
population, 分析其有啥gene 高表达,以此出发建立CreERT transgenic model来确认
新的marker.
对stem cell来说找到marker不是目的,更重要的是这个maker背后的mechanism. 一个
新的marker市时常可以揭示新的regulation mechanism.
第二个途径是逆向寻找,说来就有些盲目了。就是我说的直... 阅读全帖
w*******y
发帖数: 60932
7
Sharpie Permanent Marker Fine Tip 5 Pack $1. Quartet EnduraGlide Dry-Erase
Marker, Fine Point, 4-Marker Set, $1 free shipping with Prime (FSSS) from
Amazon
Sharpie Permanent Marker Fine Tip 5 Pack Link:
http://www.amazon.com/gp/product/B000I0TZO4/ref=pd_luc_mri?ie=UTF8&m=ATVPDKIKX0DER
Quartet EnduraGlide Dry-Erase Marker, Fine Point, 4-Marker Set, Link:
http://www.amazon.com/gp/product/B001HA0N2M/ref=pd_luc_mri?ie=UTF8&m=ATVPDKIKX0DER
h*****G
发帖数: 113
8
大家好。
做一个实验,需要co-stain cell surface marker 和intracellular protein,然后拍
照。细胞是adherent 的。
因为担心fixation和permeabilization会影响cell surface marker,所以准备先活细胞
染 cell surface marker,然后在fix,permeabilize染intracellular protein.
我的问题是可以活细胞染 cell surface marker, 马上fix,然后再染 2nd antibody
anti-cell surface marker吗?
还是必须活细胞染 cell surface marker,再染2nd antibody anti-cell surface
marker, 完了才能fix?
谢谢。
c*******e
发帖数: 514
9
来自主题: Paint_ball版 - 装备升级篇。mask, tank, marker+hopper.
今年因为groupon, google offer等网站如雨后春笋般的出现, 搞出很多paintball的
deal. 作为一个爱deal的国人, 结果就..........
上次讲过入门paintball最好的marker 就是A5+flatline barrel。 这marker射程远,
皮实,性价比又高, 继续推荐. 基本价位最好的面具还是强烈推荐JT flex 8
Thermal. 非常舒服,防雾,保护又非常全面。tank买68/4500 HPA. 几个大牌子都不错
。 我用的是Ninja Carbon fiber. 纯个人喜好,没所谓。 如果不想$100+的话说,可
以买48/30。黑猩猩的只要37。 性价比最高。 就是差不多每俩个game(500 balls)就
要refill.
如果同学也开始喜欢paintball, 开始上瘾的话。 A5可能就不能提供需要的性能。例
如如果玩speedball, 就会被其他player out gunned. 大家都ramping, marker又小又
灵活。
speedball, 中级的marker一般在$400-$500左右。有几个... 阅读全帖
s******y
发帖数: 28562
10
我觉得他的文章的意思就是大部分基因的表达程度都和细胞的活跃程度有关,
所以随便乱抓几个都能看出区别来,但是和癌症的机理基本上就是打酱油的关系。
总体意思就是目前的主流方法找出来的marker不能作为推测机理的起点。
他们在faculty 1000上抱怨说他们这篇文章一共投了4年,前后被六个
顶级杂志据了(他们直接点名的几家估计是对他们态度最糟糕的,哈哈哈)
这里是他们在PLoS Comp Biol 发表的文章的摘要。
Bridging the gap between animal or in vitro models and human disease is
essential in medical research. Researchers often suggest that a biological
mechanism is relevant to human cancer from the statistical association of a
gene expression marker (a signature) of this mechanism, that was d... 阅读全帖
s******y
发帖数: 28562
11
我觉得他的文章的意思就是大部分基因的表达程度都和细胞的活跃程度有关,
所以随便乱抓几个都能看出区别来,但是和癌症的机理基本上就是打酱油的关系。
总体意思就是目前的主流方法找出来的marker不能作为推测机理的起点。
他们在faculty 1000上抱怨说他们这篇文章一共投了4年,前后被六个
顶级杂志据了(他们直接点名的几家估计是对他们态度最糟糕的,哈哈哈)
这里是他们在PLoS Comp Biol 发表的文章的摘要。
Bridging the gap between animal or in vitro models and human disease is
essential in medical research. Researchers often suggest that a biological
mechanism is relevant to human cancer from the statistical association of a
gene expression marker (a signature) of this mechanism, that was d... 阅读全帖
w*******y
发帖数: 60932
12
Sharpie Retractable Fine Point Permanent Markers, 3 Colored Markers ( grey
body ) $1.99 free shipping with Prime (FSSS) from Amazon
Blue , Black , Red
Same great Sharpie ink, now in a fine point retractable marker that allows
easy, one-handed operation- just click and mark
Unique Safety Seal(TM) valve design prevents dry out, and there is no cap to
remove or lose
Durable fine point tip produces thinner, detailed lines
AP certified non-toxic
Set of 3 Markers includes 1 each of Black, Blue and Red... 阅读全帖
w*******y
发帖数: 60932
13
Sharpie Ultra Fine Point Permanent Markers, 12 Colored Markers (37172):
http://www.amazon.com/Sharpie-Permanent-Markers-Colored-37172/d
Sharpie Permanent Marker.
Ultra Fine Tip.
Assorted Colors.
12 Markers per Set.
Sold by THE ST
Fade-proof and water-resistant gel ink.
Acid-free.
Uni-ball premium quality performance.
h*****G
发帖数: 113
14
Thanks.
你染完cell surface marker以后马上fix,然后再染2nd anti-cell surface marker
还是染完cell surface marker,再染2nd anti-cell surface marker,fix,然后再
intracellular staining的?

marker
X******2
发帖数: 5859
15
你这个说法老夫非常赞同。
老夫觉得作者的论证有问题,100个随机挑选的marker
得到的结果优于少数“精心”挑选出来的marker
并不能说明那少数marker没有价值。
老夫曾经由于其它问题研究过在混合高斯模型下,
从高维数据中随机抽取部分特征后的“表达力”问题。
发现这随机抽取的特征只需要满足很弱的协相关
性的假设,那么这些特征的表达能力跟用全部特
征差别不大。具体取决于全部特征本身的表达能
力,以及抽取的特征数量占全部特征数量的比例。
由于这样随机抽取100 个marker以后,其表达能
力跟用全部基因的表达能力差别不大,所以这100
个marker的表达能力就很强了。因此它们会跟“精心”
挑出来的少数基因结果差不多甚至更优越。
当然话说回来,目前的很多相关研究都是有问题
的。自欺欺人地在用统计却不管任何条件和假设,
反正投稿或是写proposal都没有问题,因为关系
的作用太大了,而且裁判很多都是没有严格科学
训练而又自以为是的MD。
X******2
发帖数: 5859
16
你这个说法老夫非常赞同。
老夫觉得作者的论证有问题,100个随机挑选的marker
得到的结果优于少数“精心”挑选出来的marker
并不能说明那少数marker没有价值。
老夫曾经由于其它问题研究过在混合高斯模型下,
从高维数据中随机抽取部分特征后的“表达力”问题。
发现这随机抽取的特征只需要满足很弱的协相关
性的假设,那么这些特征的表达能力跟用全部特
征差别不大。具体取决于全部特征本身的表达能
力,以及抽取的特征数量占全部特征数量的比例。
由于这样随机抽取100 个marker以后,其表达能
力跟用全部基因的表达能力差别不大,所以这100
个marker的表达能力就很强了。因此它们会跟“精心”
挑出来的少数基因结果差不多甚至更优越。
当然话说回来,目前的很多相关研究都是有问题
的。自欺欺人地在用统计却不管任何条件和假设,
反正投稿或是写proposal都没有问题,因为关系
的作用太大了,而且裁判很多都是没有严格科学
训练而又自以为是的MD。
z****u
发帖数: 1007
17
来自主题: Biology版 - 关于stem cell marker的筛选的设想
对的,很多研究单纯就靠几个所谓marker的表达水平来鉴定stem cell identity确实不
可行。
lineage tracing不完美,比如leakage等问题。不过已经是当前能做到的最好的了。依
然该是金标准。
没有一个marker是完美的。比如alexandra joyner的那篇关于GLi1+ cell作为hair
follicle stem cell marker的文章。单纯从lineage tracing结果来看其实并不完美,
其对hair follicle的contribution并不完全,但是重要的是他们揭示了这群gli1+
cell是受神经分泌的Shh的调控的。这就重要了。marker不是目的,找到一个marker就
意味着一个in vivo model的建立以及一个可能的新的regulatory mechanism.
到底什么是stem cell.我很赞同你的观点。。stem cell 到底是个identity还是个功能
? 这个问题现在很难回答。
c******g
发帖数: 19294
18
来自主题: Paint_ball版 - [合集] 我的第一把paintball marker
【 以下文字转载自 paintball 俱乐部 】
发信人: cengceng (Yeah Yeah, Oi Oi), 信区: paintball
标 题: [合集] 我的第一把paintball marker
发信站: BBS 未名空间站 (Wed Feb 16 14:27:37 2011, 美东)
☆─────────────────────────────────────☆
cqbplayer (rover) 于 (Tue Jun 22 12:46:58 2010, 美东) 提到:
第一把marker是5年前买的,当时的预算是$150封顶。在网上找review,主要是www.
pbreview.com,先看价位合适的, 再去掉below average rating或只有几个review的。
标准大概是10个review以上,平均8.5分以上。
接下来就是到网上找deal,后来发现还是ebay的deal不错,当时ebay还不像现在这么黑,
现在的ebay简直就是逼良为娼,呵呵,跑题了。。。
spyder mr1, tippmann 98 都是经典中的经典,但是pack... 阅读全帖
w*******y
发帖数: 60932
19
Walmart has a Crayola Creativity Kit:
http://www.walmart.com/ip/Crayola-Creativity-Kit/14957014
for $10, free site to store shipping or 97 cent shipping to home.
Kids will be entertained for hours with the Crayola Creativity Kit. No other
brand is more trusted for providing safe activities for creative play.
Crayola brings it all together in the Crayola Creativity Kit, which includes
Crayola crayons, Crayola markers, pencils, silly putty, and more. For
endless creations and hours of fun, the ... 阅读全帖
w*******y
发帖数: 60932
20
12pk sharpies:
http://www.amazon.com/Sharpie-Chisel-Permanent-Markers-36201/dp
sold by amazon for $5.99. Might be listed in more buying choices on the
side.
Experience two markers in one with the revolutionary twin tip permanent
marker
Features a fine point tip for thin, detailed lines on one end and a chisel
point tip for both broad and fine line widths on the other end
Larger marker allows for a super large ink supply
Quick-drying ink is waterproof, smear proof and fade-resistant
Set of 12 Bl... 阅读全帖
w*******y
发帖数: 60932
21
Amazon has Avery Page Markers, 0.5 Inch, Bright Colors, 250 Markers:
http://www.amazon.com/Avery-Markers-Bright-Colors-22549/dp/B004
for $1.89 w/ FSSS or FS with Amazon Prime, Mom, or Student.
Not quite as good as Post-It Page Markers deal (150 markers for $1.04) last
week but these have 250 notes and come in five colors so it is better for
what I needed them for. Plus they are in stock and are really highly rated.
w*******y
发帖数: 60932
22
OfficeMax.com 100% back in MaxPerks rewards on erasers, pens, markers,
liquid pencils, dry erase markers and wipes 8/21-8/27
100% back in MaxPerks rewards on your purchase of select schoool supplies
LINK:
http://www.officemax.com/promotional-pages/maxperks-member-spec
. Log into your rewards account until OfficeMax.com page updates.
details to come
Note 00: 100% back in rewards offers combine with "buy x, get y free"
offers -- no matter what the email confirmation suggests.
Note 01: The item ... 阅读全帖
w*******y
发帖数: 60932
23
Just bought a set for myself of the fine point, but they have ultra fine as
well. Free shipping. The code will automatically be added at checkout, but
it's 99669.
SharpieUltra Fine Point Permanent Markers, Assorted, Dozen - $3:
http://www.staples.com/Sharpie-Ultra-Fine-Point-Permanent-Marke
SharpieFine Point Permanent Markers, Assorted, Dozen - $3:
http://www.staples.com/Sharpie-Fine-Point-Permanent-Markers-Ass
-Set of 12 markers includes 1 each of: black, blue, red, green, orange,
yellow, pur... 阅读全帖
s******y
发帖数: 28562
24
David Venet from the Université Libre de Bruxelles 用胡乱挑选的基因组合
和已经发表的所谓的癌基因的marker 用在未知的病人的数据上,发现胡乱挑选
的基因的准确率比那些所谓的marker 还要好。
文章结尾直接点了几个杂志的名:Cell, Nature Genetics, PNAS, 认为这些杂志
过分鼓吹所谓的 gene marker 的意义。从文章里面的行文来看,估计这些也是
把这个文章据掉的杂志 (lol)
文章最后发表在
PLoS Comput Biol. 2011 Oct;7(10):e1002240. Epub 2011 Oct 20.
http://blog.f1000.com/2012/01/10/random-gene-sets-can-predict-b
Random gene sets can predict breast cancer survival better than cancer-
related signatures
And top-tier journals did not want to pu... 阅读全帖
s******y
发帖数: 28562
25
David Venet from the Université Libre de Bruxelles 用胡乱挑选的基因组合
和已经发表的所谓的癌基因的marker 用在未知的病人的数据上,发现胡乱挑选
的基因的准确率比那些所谓的marker 还要好。
文章结尾直接点了几个杂志的名:Cell, Nature Genetics, PNAS, 认为这些杂志
过分鼓吹所谓的 gene marker 的意义。从文章里面的行文来看,估计这些也是
把这个文章据掉的杂志 (lol)
文章最后发表在
PLoS Comput Biol. 2011 Oct;7(10):e1002240. Epub 2011 Oct 20.
http://blog.f1000.com/2012/01/10/random-gene-sets-can-predict-b
Random gene sets can predict breast cancer survival better than cancer-
related signatures
And top-tier journals did not want to pu... 阅读全帖
z****u
发帖数: 1007
26
来自主题: Biology版 - 关于stem cell marker的筛选的设想
找到一个marker后能开展研究的方向很多,上下游只是一方面。
除此以外,基于这个CreERT model研究其表达pattern ,Stem cell dynamic本身就很有
意义了。比如说Hans Clevers组做的Lgr5, Lgr6在肠道里的那几篇Nature, Science文
章,就只是研究其表达位置,还有拿confetti reporter来研究其激活后populate gut
epithelium后的方式。对其上下游signal pathway基本没咋研究。
就像你说的,其实很多干细胞marker真的只是凑巧在那里表达,没啥重要的功能意义,
比如NG2,nestin,俩都是最早是在brain里面被拿来标记神经干细胞的,然后做MSC的
发现其标记MSC不错。但这俩的knockout并不会导致啥MSC的功能缺陷。还有Gli1也是。
其功能对于MSC来说很弱,有点像是凑巧在干细胞那里表达。
干细胞的marker 可以是功能性的,比如ESC里的sox2, oct4啥的。也可以是非功能性的
,这个该占大多数,比如HSC里面的很多表面标记真的和功能没有明显联系,knockout... 阅读全帖
t****w
发帖数: 130
27
来自主题: Biology版 - 关于stem cell marker的筛选的设想
仅仅依靠一个两个所谓marker基因的相对表达水平来鉴定cell identity是阻碍干细胞
领域发展的最大障碍之一。
看上去很美好的Lineage tracing系统其实问题挺多,最大的问题就是干细胞往往非常
rare,很难detect到。而你detect到大量label成功的细胞不一定是真正发挥功能的干细
胞。
今后的希望还是回归到最本质的问题:what is cell? 细胞是执行生物功能的一个功能
单位,所以cell identity还是要从功能鉴定入手。所谓stem cell,不在于是否表达
marker,而在于在体内(或体外)是否有self-renewal的能力,是否能在分化过程中产
生出其他生物学功能。
关于功能检测方法多多,可操作性强一点的就是看看几个功能相关基因的表达。硬要讨
论“marker”的话,几个功能相关的基因协同表达倒可以算是个有意义的“marker set
”。
w*******y
发帖数: 60932
w*******y
发帖数: 60932
29
OfficeMax.com 100% back in MaxPerks rewards on pens and permanent and dry
erase markers 11/07/2010 - 11/13/2010
Online Only.
NOTE: at least for me, the items are all discontinued at the moment. This
will probably change to in-stock either between 3 AM and 4AM eastern Sunday
morning.
"It's like getting FREE supplies after 100% back in MaxPerks Bonus Rewards
on select items." link:
http://www.officemax.com/promotional-pages/maxperks-member-specials/maxperks-member-specials
SharpieChisel Tip Per... 阅读全帖
w*******y
发帖数: 60932
w*******y
发帖数: 60932
w*******y
发帖数: 60932
32
Sharpie Fine-Tip Permanent Marker, 24-Pack Assorted Colors - Carribean
colors:
http://www.amazon.com/Sharpie-Fine-Tip-Permanent-24-Pack-Assort
or these ship in 2 to 4 weeks:
Sharpie Ultra-Fine-Point Permanent Markers, 24-Pack Colored Markers - 80's
Glam Colors:
http://www.amazon.com/Sharpie-Ultra-Fine-Point-Permanent-Marker
w*******y
发帖数: 60932
33
Link:
http://www.facebook.com/expomarkers?sk=app_190322544333196
like & click the coupon tab for a $2 off any 2 packs expo markers
Now through August 13th, you can score FREE Expo Washable Markers at
Walgreens (the 2-pack black markers are on sale for $.99)
Link to previous thread with different cpn for expo markers
http:// 6662
t***z
发帖数: 5112
34
还有办法去掉么?今天刚刚给孩子买了一个画画的板子,找了一个marker给儿子让他自
己画着玩。可是没注意那个是permanent marker画上以后擦不掉了才仔细看了一下笔上
面写着permanent marker。。。真是太粗心了。还有希望么?不行就只能用白纸了。。。
q******s
发帖数: 289
35
给娃买了本activity book,回来发现是dry erase的book。
好像还要买笔和擦子。上网研究了一下,还是很晕啊,好像有很多种笔呢:有dry
erase marker, wet erase marker, and washable crayon etc.
这些不同的marker到底有啥区别呢?谁能给我科普一下啊?
我的这个书是dry erase的,那其他的笔譬如washable crayon还可以在上面用吗?
对了,还有一种wipe clean的activity book,这种怎么样呢?要用什么样的笔呢?
谢谢大家了。
C*****n
发帖数: 1872
36
来自主题: Software版 - Excel绘图marker问题请教
我要绘制几条曲线,想用marker来区分,但是因为数据点多,marker都连在一起了
根本看不出来了
请问有啥办法来调整marker的间距啊?
谢谢先!
z****u
发帖数: 1007
37
多谢楼上各位意见。 我可能会尝试COUP-TFII。因为samople是H2B-GFP表达老鼠,需要
GFP+arteriole marker. ephrinB2 staining如果过太复杂或者涉及antigen retrieval
,GFP可能会受影响。
另外我找到一个smoothelin 文献说特异表达在arteriole的SMC里,而不在venule的SMC
.
形态学上动脉和静脉还是很好区分的。但是我想最好还是用多个marker以及形态学一起
来验证我的GFP-expressing cell的位置。所以才在寻找这些marker
a********k
发帖数: 2273
38
扫了一眼
大意应该是随便抓100个以上的基因,(包括那些发表的看了joke表达不同的,social
有问题的,皮肤细胞问题的基因)90%的可能cancer和正常细胞的表达谱就会有区别。
作者抓了几十篇paper里面报道的所谓在breast cancer里面表达有区别的基因,和他随
便抓的基因比较,只要抓到100个基因以上,两个结果就相差不显著了。
然后他看了一下发表的那些所谓marker,大部分都和cell cycle/PCNA有关,如果拿
PCNA的表达做normalization,世界清静了,cancer marker基本失效。
结论就是cancer marker就是看细胞分裂的,没啥奇怪。
s******s
发帖数: 13035
39
我没看原文。如果别人只是几个或者十几个marker, 他搞一个100基因marker,
那是没啥可比的。就算都是100个基因的marker, 这么多dummy variable几乎
可以解释到极限了,其他的“未必”能有很大的提高。
a********k
发帖数: 2273
40
扫了一眼
大意应该是随便抓100个以上的基因,(包括那些发表的看了joke表达不同的,social
有问题的,皮肤细胞问题的基因)90%的可能cancer和正常细胞的表达谱就会有区别。
作者抓了几十篇paper里面报道的所谓在breast cancer里面表达有区别的基因,和他随
便抓的基因比较,只要抓到100个基因以上,两个结果就相差不显著了。
然后他看了一下发表的那些所谓marker,大部分都和cell cycle/PCNA有关,如果拿
PCNA的表达做normalization,世界清静了,cancer marker基本失效。
结论就是cancer marker就是看细胞分裂的,没啥奇怪。
s******s
发帖数: 13035
41
我没看原文。如果别人只是几个或者十几个marker, 他搞一个100基因marker,
那是没啥可比的。就算都是100个基因的marker, 这么多dummy variable几乎
可以解释到极限了,其他的“未必”能有很大的提高。
h******y
发帖数: 351
42
来自主题: Biology版 - Senescence Marker
是啊,现在大家常用的几个marker都有这样或那样的缺陷。
SA-b-Gal方便,但是假阳性挺高。在培养细胞中用起来挺方便,看组织切片就困难多了。
SAHF,rH2A.X,53BP1也会有DNA Damage的假阳性。
Ki67,BrdU是negative marker,更不好说了。
谁要是找到一个specific的marker就好了
w*******y
发帖数: 60932
w*******y
发帖数: 60932
44
Quartet (5001-12M) EnduraGlide Dry Erase Markers for Whiteboards, 4-Pack,
Black $1.83 free shipping with Prime (FSSS) from Amazon
Thought these might come in handy for the recent White Board deal
4 Pack Black Markers
See-thru ink barrel gauge
Non-toxic, low odor
Whiteboard safe
Link:
http://www.amazon.com/Quartet-5001-12M-EnduraGlide-Markers-Whiteboards/dp/B000J07IX8
w*******y
发帖数: 60932
45
Link:
http://www.walmart.com/ip/Color-Wonder-Magic-Light-Up-Markers/13907187
I have never used these before, but for the price, I figured I couldn't go
wrong. I ordered 2 and with tax it was 4.07 shipped to my house.
Standard Shipping
Items Qty Arrival Date Price
Color Wonder Magic Light Up Markers 2 Will arrive between Thursday, 11/18/
10, and Friday, 11/19/10. $1.00
Subtotal: $2.00
Shipping: $1.94
Tax: $0.13
Walmart.com Total: $4.07
Same item on amazon is 13.97
Link:
http://www.amazon.c... 阅读全帖
w*******y
发帖数: 60932
w*******y
发帖数: 60932
47
I searched for "Staples Markers" and couldn't find this deal posted!
Link:
http://www.staples.com/Quartet-EnduraGlide-Chisel-Tip-Dry-Erase-Markers-Black-Dozen/product_805512
QuartetEnduraGlide Chisel Tip Dry-Erase Markers, Black, Dozen
Reg. $14.99
Sale $9.74
MINUS COUPON of $6
Makes it a total: 3.74
Patented 3-chamber system with barrel gauge that indicates when ink levels
are low
Low-odor, quick-drying ink designed to reduce smearing yet erases easily
Black and "Assorted colors: 3 black, 3 blue... 阅读全帖
w*******y
发帖数: 60932
48
Sharpie Metallic Fine Point Permanent Markers, 2 Silver Markers (39108PP)
Great reviews, I have personally used it and it works great. This is the
best price I have found.
LINK
w*******y
发帖数: 60932
49
Link:
http://www.coupons.com/couponweb/Offers.aspx?pid=14997&zid=iq55
Coupons.com just released an awesome new coupon valid for $2.00 off ANY 2
Packs of Expo Washable Markers! Now through August 13th, you can score FREE
Expo Washable Markers at Walgreens!
Check zip 45176 and select Office Supply category to quickly locate this
coupon.
earlier post showing 4-pack markers on sale for $1,99
http:// 3940
w*******y
发帖数: 60932
50
OfficeMax 100% back in MaxPerks rewards on select view binders, mechanical
pencils, pens, wet or dry erase markers and correction film 8/14-8/20
100% back in MaxPerks rewards on your purchase of select supplies link:
http://www.officemax.com/promotional-pages/maxperks-member-spec
.
see linked ad. Details to follow.
Note 01: The item maybe be "discontinued" until it is made available
forsale (usually around 3 AM eastern). The "discontinued" state can occur
sporadically through out the day on ... 阅读全帖
1 2 3 4 5 6 7 8 9 10 下页 末页 (共10页)