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全部话题 - 话题: method
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y*****w
发帖数: 146
1
来自主题: Biology版 - 急,paper help, Methods in Enzymology
Methods in Enzymology
Vectors for expression of cloned genes in yeast: regulation, overproduction,
and underproduction.
Schneider JC, Guarente L.
PMID: 2005797
Links:
http://chemport.cas.org/cgi-bin/sdcgi?APP=cp_scifinder&SERVICE=
or
http://www.sciencedirect.com/science/article/pii/00766879919402
Thanks a lot for the help!
y******[email protected]
z*******6
发帖数: 679
2
来自主题: Biology版 - Paper help... Nature method...
太土了,没想到连nature method都没订...
http://www.ncbi.nlm.nih.gov/pubmed/16554834
n******[email protected]
谢啦!!
T*********l
发帖数: 298
3
Methods in Molecular Biology
2012, Volume 926, 317-329,
DOI: 10.1007/978-1-62703-002-1_20
Title:
Application of embryonic and adult zebrafish for nanotoxicity assessment.
email: g*******[email protected]
有包子!谢谢!
a***n
发帖数: 92
4
Does anyone has access to the following paper?
J Neurosci Methods. 1991 Mar;37(1):15-26.
Low access resistance perforated patch recordings using amphotericin B.
Rae J, Cooper K, Gates P, Watsky M.
http://www.ncbi.nlm.nih.gov/pubmed/2072734
Could you send me a copy to a******[email protected]
Thank you very much for your help!
Happy New Year!
h****n
发帖数: 2552
5
来自主题: Biology版 - 包子求篇nature methods的文章
Dekker J (2006) The three ‘C’ s of chromosome conformation capture:
controls, controls,controls. Nat Methods 3:17–21.
多谢!
j******i
发帖数: 939
6
Nature系列有transfer服务吧 格式也不用改 我也在准备尝试其中一个 Nature
methods和Nature Biotechnology 国人发的好像比cns还少 有点技术原创滞后发现原创
味道
s******n
发帖数: 110
7
来自主题: Biology版 - PLease help for the statistical methods!
Every biological expert:
Please help me for the stat method in the attachment.
DO you think it should be use 1-way or 2-way ANOVA?
Thanks a lot
j*m
发帖数: 6
8
来自主题: Biology版 - 新杂志 J Biol Methods, 请捧场
基于有10多年历史的Protocol Online和BioForum, 我们创办了一本Open access的新期
刊,叫 Journal of Biological Methods http://www.jbmethods.org。希望等到大家的支持。谢谢。
如有意成为审稿人,请注册并注明专长领域。
l****m
发帖数: 751
9
如果热门领域竞争激烈就一起发了。
如果合起来只是结果好,对文章的档次没有明显提升,不赶时间就慢慢灌2篇。
如果合起来文章能升一个档次,比如从10升到nature methods就一起发。
a*q
发帖数: 2109
10
做Imaging还有Proteomics的基本上除了CNS就是Nature Methods了(还有Nature
Biotechnology)。
m****z
发帖数: 5
11
你从哪里得出的结论做proteomics 除了CNS就是Nat Methods?
t***q
发帖数: 65
12
他说的属实,我们组里一个zero productivity 的博后搞Proteomics,号称自己phD阶
段的文章会在
Nat Methods发表。跟我们这么说已经3年了
h********n
发帖数: 4079
13
not true
Nature Methods is my favorite.
k*****n
发帖数: 417
14
来自主题: Biology版 - Sunney Xie评上method of the year
http://news.sciencenet.cn/htmlnews/2014/1/287264.shtm
2014年1月《自然—方法学》(Nature Methods)上发表年度特别报道,将“单细胞测
序”(Singled out for sequencing)的应用列为2013年度最重要的方法学进展。
近几年来,基于单细胞测序技术的科学研究取得了突飞猛进的发展,其成果有望为一些
重要的医学问题提供新的解决方案。文章总结了2013年单细胞测序技术对于人类早期发
育、癌症以及神经科学研究等几个重点领域的最新应用成果。文章特别指出,来自中国
北京大学的一些研究团队在这方面完成了许多优秀的工作,做出了突出的贡献。
单细胞基因组扩增新技术(MALBAC)最早由哈佛大学谢晓亮教授发明,相关论文2012年
发表于《科学》 (Science)杂志。该方法通过形成闭合环来抑制DNA片段被重复地复
制,以保持DNA扩增的均匀性,解决了传统方法对单细胞基因组扩增的强烈偏好性的问
题。这项突破在单个细胞水平实现了全基因组93%的高覆盖率,同时也能准确检测单个
肿瘤细胞中的染色体拷贝数异常。
2013年是单... 阅读全帖
U**l
发帖数: 153
15
来自主题: Biology版 - Sunney Xie评上method of the year
国内宣传做得真快。
Nature methods 上其实还提到了UCSD的 Kun Zhang lab做的用 microfluidics 实现的
单细胞分离和测序。好像是去年nature biotech的文章。感觉办法很直接很高效的,不
知道成本是否会高。 不过sunney Xie的办法肯定不是唯一的。

★ 发自iPhone App: ChineseWeb 8.2.2
c**********n
发帖数: 177
f**********r
发帖数: 18251
17
各位也是言必称CNS的,当然很多也是CNS灌水牛人
庄在CNS上也是一直灌水的,06年那时就经常灌了,怎么这个如此重要的发现就这么谦
虚,直接投了NATURE METHOD
s******8
发帖数: 2131
18
Nature Methods 是新的吗?和Nature Communications, Scientific Reports,
Science Advances 比较?
m*******s
发帖数: 3142
19
【 以下文字转载自 Physics 讨论区 】
发信人: slowfish (catCat), 信区: Physics
标 题: 求本书:The Embedding Method for Electronic Structure
发信站: BBS 未名空间站 (Mon Jan 2 09:17:33 2017, 美东)
http://iopscience.iop.org/book/978-0-7503-1042-0
学校图书馆么订,哪位大神可以下下来的,请发至: [email protected]/* */
感激不尽~~~
S*R
发帖数: 1057
20
2017年5月31日,国际学术权威刊物自然出版集团旗下子刊《Nature Methods》杂志在
线发表了北京大学程和平教授研究组的一篇研究论文,研究人员研制出新一代微型化双
光子荧光显微镜,重量仅2.2克,可佩戴在动物头部,实时记录数十个神经元和上千个
神经突触的动态信号;横向分辨率可达0.65微米,成像质量可与商品化大型台式双光子
荧光显微镜相媲美。
人们观察大脑多通过解剖,大脑处在静止、无生命的状态。程和平教授说,新一代微型
化双光子荧光显微成像改变了观察细胞、亚细胞结构的方式。可在动物觅食、哺乳、打
斗、嬉戏、睡眠等自然行为下,或在学习前、中、后长时间观察神经突触、神经元、神
经网络、远程连接的脑区等多尺度、多层次动态变化。
据介绍,该显微镜成像分辨率高、速度快,并具备多区域随机扫描和每秒1万线的线扫
描能力;采用柔性光纤束进行荧光信号接收,解决了动物活动、行为由于荧光传输光缆
拖拽而受到干扰等难题。其效果远优于目前领域内主导的由美国脑科学计划核心团队所
研发的微型化宽场显微镜。
程和平说,该显微镜应用领域广阔。如在外科手术临床应用上,做开颅手术前就可看到
脑部肿瘤边界和神经活... 阅读全帖
s****w
发帖数: 10
21
我个人对consumer behavior更感兴趣点,但看了下课程,觉得没什么实用价值。反而
quantitative method看起来更适合进industry. 如果这俩能和一块儿该有多好啊~~~
有人说说嘛?
s*****t
发帖数: 71
22
有个分析的东西,感觉投analytical chem有点亏,想试试nature method,不知道有没
有同学对这两个杂志有了解的,nature mathod上分析的东西是不是比analytical chem
水平高很多?
s*****t
发帖数: 71
23
谢谢楼上同学。看来还是老老实实的整analytical chem吧,
因为我看到nature method上一些东西也就那回事,本来想试试的
J*******3
发帖数: 1651
24
Terribly sorry. It is : Richard Martin: Electronic Structure: Basic Theory
and Practical Methods (Vol 1)
Thank you very much!
a*y
发帖数: 54
25
http://www.nature.com/nmeth/journal/v9/n11/full/nmeth.2185.html
A microfluidic device and computational platform for high-throughput live
imaging of gene expression
Nature Methods 9,1101–1106(2012)doi:10.1038/nmeth.2185
Please send the file to y****[email protected]. I appreciate your help very much.
h***o
发帖数: 350
26
来自主题: Chemistry版 - paper help (Analytical Methods)
Real-time detection of chemical warfare agent simulants in forensic samples
using active capillary plasma ionization with benchtop and field-deployable
mass spectrometers
Morphy Dumlao, Pablo Martinez-Lozano Sinues, Maryia Nudnova and Renato
Zenobi
Anal. Methods, 2014,6, 3604-3609
DOI: 10.1039/C4AY00303A
Link:
http://pubs.rsc.org/en/Content/ArticleLanding/2014/AY/C4AY00303
Thank you very much.
m*******s
发帖数: 3142
27
【 以下文字转载自 Physics 讨论区 】
发信人: slowfish (catCat), 信区: Physics
标 题: 求本书:The Embedding Method for Electronic Structure
发信站: BBS 未名空间站 (Mon Jan 2 09:17:33 2017, 美东)
http://iopscience.iop.org/book/978-0-7503-1042-0
学校图书馆么订,哪位大神可以下下来的,请发至: [email protected]/* */
感激不尽~~~
s********1
发帖数: 581
28
有关书籍“numerical methods for scientists and engineers”???
本人找到至少三本完全不同的书,作者和时间完全不一样。请问那提本才是最好???
1. Richard Hamming
2. H.M. Antia
3.Joe D. Hoffman
A*******h
发帖数: 142
29
来自主题: Computation版 - Linear Search method for CG?
用Conjugate Gradient优化时最有效的Linear Search method是那一种?
有没有不用计算first derivative的?
b*****d
发帖数: 62
30
来自主题: Computation版 - reference book on interior-point methods
Can anyone recommend any good reference book/textbook on inter-point methods
for mathematical programing/optimization? Is Steve Wright's book good? Thanks!
P******g
发帖数: 12
31
有谁知道横共振法( transverse resonant method)具体内容是什么吗?在那里可以找
到详细的介绍? 谢谢!
s*s
发帖数: 6
32


We applied this method to determine the cutoff mode of a dielectric waveguide.
You can find it in some traditional electromagnetic books.
o******p
发帖数: 3
33
【 以下文字转载自 Mathematics 讨论区,原文如下 】
发信人: outstrip (一生一次), 信区: Mathematics
标 题: Numerical methods for calculating area
发信站: Unknown Space - 未名空间 (Sat May 22 21:42:16 2004) WWW-POST
I need to calculate the area of a surface represented as a mesh.
I also want to calculate weighted area of the mesh, that is, each node in the
mesh has a weight.
Anyone who can provide some suggestions is highly appreciated.
s********1
发帖数: 581
34
有关书籍“numerical methods for scientists and engineers”???
本人找到至少三本完全不同的书,作者和时间完全不一样。请问那提本才是最好???
1. Richard Hamming
2. H.M. Antia
3.Joe D. Hoffman
h****r
发帖数: 258
35
来自主题: Computation版 - ALE 和 Level-Set Method

Agree with the above. These two reside in different application domain.
For level set, I'd recommend the bible "Level set methods and dynamic
implicit surfaces" by Osher and Fedkiw.
i******t
发帖数: 370
36
Check the condition number of your Hessian first. If your Hessian is ill-
conditioned in the last steps, the convergence could be bad.
Also Newton's method could fail to converge if starting too far away from
the optimum.
i******t
发帖数: 370
37
The function value increases probably because the second order
approximation (the assumption of Newton's method) is really bad there - in
the extreme case it could diverge. Not necessarily because the code has bugs.

with the case when the hessian matrix is not positive definite?
z*****a
发帖数: 3809
38
I was assuming Newton's method is only used to generate the step direction
and a line search is used to compute the step size.

in
bugs.
w***h
发帖数: 415
39
手头有一本电子书(1.5M), 需要者留下收件的电邮.
手续费两个包子(20买卖提伪币), 以表示对鄙人的感谢, 呵呵.
电子书信息:
Texts in Applied Mathematics 45
Stig Larsson · Vidar Thomée
"Partial Differential Equations with Numerical Methods"
First softcover printing 2009
ISBN 978-3-540-88705-8 e-ISBN 978-3-540-88706-5
DOI 10.1007/978-3-540-88706-5
Texts in Applied Mathematics ISSN 0939-2475
Library of Congress Control Number: 2008940064
Mathematics Subject Classification (2000): 35-01, 65-01
2009, 2003 Springer-Verlag Berlin Heidelberg

发帖数: 1
40
http://www.mdpi.com/journal/remotesensing/special_issues/rs_bigdata
• Fundamental theories for remote sensing data processing, such as
data representation, data clean, dimension reduction, feature selection,
compressive sensing, deep learning, regression, correlation analysis, data
organization and structure, etc.;
• Methods and techniques for collection, distribution, sharing, and
visualization of remote sensing big data;
• Remote sensing big data processing infrastructures an... 阅读全帖

发帖数: 1
41
http://www.mdpi.com/journal/remotesensing/special_issues/rs_bigdata
• Fundamental theories for remote sensing data processing, such as
data representation, data clean, dimension reduction, feature selection,
compressive sensing, deep learning, regression, correlation analysis, data
organization and structure, etc.;
• Methods and techniques for collection, distribution, sharing, and
visualization of remote sensing big data;
• Remote sensing big data processing infrastructures an... 阅读全帖
F******l
发帖数: 25
42
来自主题: Economics版 - Quantative Method Business/OR
请教这里的朋友,Quantative Method Business/OR(operating research) 这个方向,若
找工作,最match的应该是什么样公司和职位?
这里有持有这样背景,找到工作的朋友么?是否可以分享一下下经验?
谢谢
b****b
发帖数: 199
43
来自主题: Economics版 - Quantative Method Business/OR
OR is much more versatile and more applicable. It would be even more useful if
working in China because the potential application of OR in chinese industry
is tremendous. I certainly will choose OR with no doubt. Many similar programs
also teach and specialize in OR: industrial engineer, operational management,
etc.
For studying quantative method business, you might be facing competition from
every quantative and statistical programs. Useful but not very specific.

p********a
发帖数: 2
44
by using exponential tilling you can contracts a
more efficient estimator (the variance is lower, the confidence
interval in more narrow)
请教为什么Monte Carlo Method方法计算expected shortfall改成Exponential
Tilting计算expected shortfall之后,方差更小和置信区间更窄?
谢谢!
c*******e
发帖数: 150
45
Glasserman's book Chap 4 has elaborate explanation on this issue. If you
are employing Monte Carlo method in your research, Glasserman's book is
an excellent text I would highly recommend.
l****y
发帖数: 96
46
我近日收到 WISC 教育系的Quantitative Methods
PHD的offer.但不知道就业前景如何. 毕业后去学校
教书好找工作吗?除此之外还有出路吗? 请前辈们指点一下啊!
先谢谢啦!
p********1
发帖数: 1011
47
有没有教的比较生动有趣的 Research Methods (social science)的lecture视频推荐

要onsite了。
e***7
发帖数: 118
48
跪求IEEE paper:Application of Design of Experiments (DOE) methods to high-
speed interconnect validation by Adam Norman from Intel.
学校没有subscribe IEEE的paper,希望版上的大侠能帮忙下载,感激不尽。
小弟邮箱是c*******[email protected]
感谢感谢。

发帖数: 1
49
http://www.mdpi.com/journal/remotesensing/special_issues/rs_bigdata
• Fundamental theories for remote sensing data processing, such as
data representation, data clean, dimension reduction, feature selection,
compressive sensing, deep learning, regression, correlation analysis, data
organization and structure, etc.;
• Methods and techniques for collection, distribution, sharing, and
visualization of remote sensing big data;
• Remote sensing big data processing infrastructures an... 阅读全帖

发帖数: 1
50
http://www.mdpi.com/journal/remotesensing/special_issues/rs_bigdata
• Fundamental theories for remote sensing data processing, such as
data representation, data clean, dimension reduction, feature selection,
compressive sensing, deep learning, regression, correlation analysis, data
organization and structure, etc.;
• Methods and techniques for collection, distribution, sharing, and
visualization of remote sensing big data;
• Remote sensing big data processing infrastructures an... 阅读全帖
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