l**********1
发帖数: 5204 | 1 De rien.
plus this one:
Shioda N et al. (2012)
Expression of a Truncated Form of the Endoplasmic Reticulum Chaperone
Protein, σ1 Receptor, Promotes Mitochondrial Energy Depletion and Apoptosis
JBC 287: 23318-31.
Abstract
The σ1 receptor (σ(1)R) regulates endoplasmic reticulum (ER)/mitochondrial
interorganellar Ca(2+) mobilization through the inositol 1,4,5-
trisphosphate receptor (IP(3)R). Here, we observed that expression of a
novel splice variant of σ(1)R, termed short form σ(1)R (σ(1)SR), has... 阅读全帖 |
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l**********1
发帖数: 5204 | 2 En
one aspect:
To keep stability and safety of Somatic cell Reprogramming to iPS cell
pls refer
Hamada M. et al. (2012)
Reprogramming to Pluripotency Can Conceal Somatic Cell Chromosomal
Instability.
PLoS Genet. 8: e1002913.
Abstract
ignored
These findings suggest that aneuploid cells can be selected for or against
during reprogramming depending on the W-CIN gene defect and present the
novel concept that somatic cell W-CIN can be concealed in the pluripotent
state. Thus, karyotypic analysis of s... 阅读全帖 |
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l**********1
发帖数: 5204 | 3 microfluidic platforms plus nanopore
pls refer
1)
Choi JW et al. (2012)
High-Throughput Analysis of Protein–Protein Interactions in Picoliter-
Volume Droplets Using Fluorescence Polarization
Anal. Chem. 84: 3849–3854
http://pubs.acs.org/doi/abs/10.1021/ac300414g
2)
Rudenko MI et al. (2011)
Controlled gating and electrical detection of single 50S ribosomal subunits
through a solid-state nanopore in a microfluidic chip.
Biosens Bioelectron. 29: 34-9.
http://www.ncbi.nlm.nih.gov/pubmed/21855314
3)
... 阅读全帖 |
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l**********1
发帖数: 5204 | 5 生物统计的入门教材之一 哈 of course english version:
http://www.roma1.infn.it/~dagos/rpp/
Introduction
Uncertainty and probability
Rules of probability
Probability of simple propositions
Probability of complete classes
Probability rules for uncertain variables
Bayesian inference for simple problems
Background information
Bayes' theorem
Inference for simple hypotheses
Inferring numerical values of physics quantities -- General ideas and basic
examples
Bayesian inference on uncertain variables and posterior ... 阅读全帖 |
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b***s
发帖数: 14 | 6 先看看无数的CNS是什么就知道了，尤其第一作者
列子先：http://www.ncbi.nlm.nih.gov/pubmed/18267069
Oncogenic BRAF Induces Senescence and Apoptosis through Pathways Mediated by
the Secreted Protein IGFBP7
Narendra Wajapeyee1, Ryan W. Serra1, Xiaochun Zhu1, Meera Mahalingam2 and
Michael R. Green1, Go To Corresponding Author,
http://www.ncbi.nlm.nih.gov/pubmed/20478260
Cell. 2010 May 14;141(4):717-27.
IGFBP7 is not required for B-RAF-induced melanocyte senescence. |
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b***s
发帖数: 14 | 7 先看看无数的CNS是什么就知道了，尤其第一作者
列子先：http://www.ncbi.nlm.nih.gov/pubmed/18267069
Oncogenic BRAF Induces Senescence and Apoptosis through Pathways Mediated by
the Secreted Protein IGFBP7
Narendra Wajapeyee1, Ryan W. Serra1, Xiaochun Zhu1, Meera Mahalingam2 and
Michael R. Green1, Go To Corresponding Author,
http://www.ncbi.nlm.nih.gov/pubmed/20478260
Cell. 2010 May 14;141(4):717-27.
IGFBP7 is not required for B-RAF-induced melanocyte senescence. |
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l**********1
发帖数: 5204 | 10 Re LZ
pls go to
one RNA-seq blog
one topic:
Long noncoding RNAs in pathogenesis
web link:
http://www.rna-seqblog.com/
LncRNAs (pronounced “link”) are long non-coding RNAs that are emerging as
important regulators of gene expression in biological processes and diseases
. In this issue of the Journal of Clinical Investigation, two papers connect
lncRNAs to inherited conditions in humans.
Sylvia B-hring and colleagues at the Experimental and Clinical Research
Center in Berlin found a chromosomal ... 阅读全帖 |
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a******a
发帖数: 283 | 11 please email to v*******[email protected], thanks alot!
pubmed link:
http://www.ncbi.nlm.nih.gov/pubmed/20384524
title
Rexin-G, a targeted genetic medicine for cancer
May 2010, Vol. 10, No. 5 , Pages 819-832 (doi:10.1517/14712598.2010.481666)
Authors info
Erlinda M Gordon†1 MD Medical Director & Frederick L Hall2 PhD Chief
Scientific Officer
1Epeius Biotechnologies Corporation, 475 Huntington Drive, San Marino, CA
91108, USA +1 626 441 6695; +1 626 441 6692; e*****[email protected]
2Epeius Bio... 阅读全帖 |
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S*****r
发帖数: 139 | 14 Title: Quantitative analysis of transcriptional pausing by Escherichia coli
RNA polymerase: his leader pause site as paradigm
Authors: Robert Landick, Daguang Wang, Cathleen L. Chan
Volume 274, 1996, Pages 334–353
Pubmed: http://www.ncbi.nlm.nih.gov/pubmed?term=8902817
please send to x*********[email protected]
Thank you!! |
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l**********1
发帖数: 5204 | 15 不要只看到Bayesian+Markov Chain Monte Carlo (MCMC) 用于NGS sensitivity
analysis
新的非 Bayesian+MCMC的 Fisher Information Matrix (FIM) sensitivity
analysis 已有英国 帝国理工大的论文了吧 吧
Reference:
i,
Bioinformatics. (2012) 28:731-3.
web link:
HTTP://www.ncbi.nlm.nih.gov/pubmed/22378710
PDF file:
HTTP: //www.theosysbio.bio.ic.ac.uk/wp-content/uploads/2011/04/manual1.pdf
就是由减少Stochastic simulation process 中的参数 数量的 视点入手的
关联论文
ii,
J R Soc Interface. (2008) 5 Suppl 1:S59-69.
HTTP: //www.ncbi.nlm.nih.gov/pubmed/1848... 阅读全帖 |
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l**********1
发帖数: 5204 | 16 Moser C, Gupta M. (2012)
A generalized hidden Markov model for determining sequence-based predictors
of nucleosome positioning.
Stat Appl Genet Mol Biol. 11 doi: 10.2202/1544-6115.1707.
HTTP: //www.ncbi.nlm.nih.gov/pubmed/22499697
plus one Nature sisiter review
Davey JW et al. (2011)
Genome-wide genetic marker discovery and genotyping using next-generation
sequencing.
Nat Rev Genet. 12: 499-510.
HTTP: //www.ncbi.nlm.nih.gov/pubmed/21681211 |
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l**********1
发帖数: 5204 | 18 Conti:
plus
Li G et al. (2011).
HDAC6 α-tubulin deacetylase: a potential therapeutic target in
neurodegenerative diseases.
J Neurol Sci. 304: 1-8.
HTTP: //www.ncbi.nlm.nih.gov/pubmed/21377170
and
Mitra G et al. (2007).
Chaperone-mediated inhibition of tubulin self-assembly.
Proteins. 67: 112-20.
HTTP: //www.ncbi.nlm.nih.gov/pubmed/17243182 |
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l**********1
发帖数: 5204 | 19 Sure
plus one Plos Bio 2012 paper from that Tom Maniatis lab too:
Zhao M. xxx mmm nnn and Tom Maniatis (2012)
Stochastic Expression of the Interferon-β Gene
PLoS Biol. 10: e1001249.
HTTP : //www.ncbi.nlm.nih.gov/pubmed/22617958
plus
2012 one Nature sister:
Multi-layered stochasticity and paracrine signal propagation shape the type-
I interferon response.
HTTP: //www.ncbi.nlm.nih.gov/pubmed/22291574
ps: for how to Stochastic Hidden Markov chain simulating MAVS KEGG pathway
pls just try one R&Pyt... 阅读全帖 |
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l**********1
发帖数: 5204 | 21 hidden markov chain model stochastic simulation
可能要用到
pls refer
Gilchrist DA et al. (2010).
Pausing of RNA polymerase II disrupts DNA-specified nucleosome organization
to enable precise gene regulation.
Cell. 143: 540-51.
HTTP: //www.ncbi.nlm.nih.gov/pubmed/21074046
and
Boettiger AN et al. (2011).
Transcriptional regulation: effects of promoter proximal pausing on speed,
synchrony and reliability.
PLoS Comput Biol.7: e1001136.
HTTP: //www.ncbi.nlm.nih.gov/pubmed/21589887 |
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l**********1
发帖数: 5204 | 22 Co-ask LZ
Is it an inhibitor of mammalian estrogen receptor?
or enhancer of USP-19
If either answer is Bingo,
pls refer
Ogawa M et al. (2011).
17β-estradiol represses myogenic differentiation by increasing ubiquitin-
specific peptidase 19 through estrogen receptor α.
JBC 286(48):41455-65.
HTTP: //www.ncbi.nlm.nih.gov/pubmed/21971047
or
Sundaram P et al. (2009).
USP19-deubiquitinating enzyme regulates levels of major myofibrillar
proteins in L6 muscle cells.
Am J Physiol Endocrinol Metab. 297: E1... 阅读全帖 |
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l**********1
发帖数: 5204 | 23 by
Kumacheva E.
Nat Mater. 11: 665-6.
Hydrogels: The catalytic curtsey.
PubMed link:
HTTP: //www.ncbi.nlm.nih.gov/pubmed/22825020
or
HTTP: //www.nature.com/nmat/journal/v11/n8/full/nmat3381.html
pls send to EM post
THX in advance! |
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g*******e
发帖数: 156 | 24 One-step assembly in yeast of 25 overlapping DNA fragments to form a
complete synthetic Mycoplasma genitalium genome
http://www.pnas.org/content/105/51/20404.full
Assembling large DNA segments in yeast.
www.ncbi.nlm.nih.gov/pubmed/22328431
Oligonucleotide assembly in yeast to produce synthetic DNA ...
www.ncbi.nlm.nih.gov/pubmed/22328422
貌似转化方法大多用原生质体转化或者醋酸锂法
问题是为啥不用快捷的电转化法？？？
谢谢！ |
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l**********1
发帖数: 5204 | 25 RE:
adeno (天夺其魄) 的post:
不是吧,
Jennifer A. Doudna HHMI
UC Berkeley组 &瑞典于默奥大的 Charpentier组的 manuscript 被 Sci magazine
accepted for publication
2012 summer n天后的
conference 上 Emma Charpentier (she) will like to note it, isn't it?
Feng Zhang 的2013 Sci magazine paper adeno (天夺其魄) 您看了全文后
看了全部references 它们的whole abstract parts 了吗?
pls refer
Multiplex Genome Engineering Using CRISPR/Cas Systems
Science. (2013) 339: 819-23.
by
Le Cong F. et al. and Feng Zhang
web link:
>HTTP://www.ncbi.nlm.nih... 阅读全帖 |
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l**********1
发帖数: 5204 | 26 RE:
adeno (天夺其魄) 的post:
不是吧,
Jennifer A. Doudna HHMI
UC Berkeley组 &瑞典于默奥大的 Charpentier组的 manuscript 被 Sci magazine
accepted for publication
2012 summer n天后的
conference 上 Emma Charpentier (she) will like to note it, isn't it?
Feng Zhang 的2013 Sci magazine paper adeno (天夺其魄) 您看了全文后
看了全部references 它们的whole abstract parts 了吗?
pls refer
Multiplex Genome Engineering Using CRISPR/Cas Systems
Science. (2013) 339: 819-23.
by
Le Cong F. et al. and Feng Zhang
web link:
>HTTP://www.ncbi.nlm.nih... 阅读全帖 |
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l**********1
发帖数: 5204 | 30 here you go
PMID 20410883
Gao Z
An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA
methylation. (2010).
Nature. 465: 106-9.
http://www.ncbi.nlm.nih.gov/pubmed/20410883
or
PMID 22895250
Zhang H, Zhu JK.
Seeing the forest for the trees: a wide perspective on RNA-directed DNA
methylation. (2012).
Genes Dev. 26: 1769-73.
http://www.ncbi.nlm.nih.gov/pubmed/22895250
or mammalian EStemCell mCXG
mCXG occurs asymmetrically with 98% of sites being methylated on only one
strand.
... 阅读全帖 |
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J*R
发帖数: 187 | 32 Dear Colleagues,
You're cordially to submit a review article for consideration at our new
journal, The Journal of Postdoctoral Research, JPR. JPR has gone live with
many papers published in a range of disciplines. Please check out http://www.postdocjournal.com/jpr.aspx.
As you may be aware, this is an ambitious initiative to develop an iconic
international peer-reviewed publication reflective of the high quality of
postdoctoral contribution to ongoing research in ANY field. The eventual
goal of ... 阅读全帖 |
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l**********1
发帖数: 5204 | 33 how about COL4A1 ?
PMID 22253229
Assadian S et al.
p53 inhibits angiogenesis by inducing the production of Arresten. (2012).
Cancer Res. 72: 1270-9.
cited
p53 directly activated transcription of the COL4A1 gene by binding to an
enhancer region 26 kbp downstream of its 3' end
PubMed link:
http://www.ncbi.nlm.nih.gov/pubmed/22253229 |
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l**********1
发帖数: 5204 | 34 Bingo bmw well said
LZ
plus one paper:
Wiggins CM et al.
BIMEL, an intrinsically disordered protein, is degraded
by 20S proteasomes in the absence of poly-ubiquitylation. (2011).
J Cell Sci. 124: 969-77.
http://www.ncbi.nlm.nih.gov/pubmed/21378313
PDF link:
//www2.mrc-lmb.cam.ac.uk/groups/dk/DK-lab/Publications_files/Wiggins-etal_
BIMELdegrad+supp.pdf
or one review
Wang Z et al.
Identification and characterization of two splicing variants of human Noxa.
(2008).
Anticancer Res. 28: 1667-74.
>The... 阅读全帖 |
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s***m
发帖数: 6197 | 36 Colloc'h, Nathalie, et al. "Comparison of three algorithms for the
assignment of secondary structure in proteins:
the advantages of a consensus assignment." Protein engineering 6.4 (1993):
377-382.
Pubmed link:
http://www.ncbi.nlm.nih.gov/pubmed/8332595
Thanks in advance!
My email address is x****[email protected]. |
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l**********1
发帖数: 5204 | 37 pls focus on protein folding analysis by Solid NMR new method
References:
PMID 22522262
Nat Chem. 4:410-7. (2012)
protein fold in the solid phase via measurements of nuclear paramagnetic
relaxation enhancements (PREs) in several analogues of the protein of
interest containing covalently attached paramagnetic tags, without the use
of conventional internuclear distance restraints.
then from
the paucity of distance restraints >5 Å
to
B1 immunoglobulin-binding domain of protein G, for which ~23... 阅读全帖 |
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k****s
发帖数: 1209 | 39 原文在
http://www.skizit.biz/2013/07/21/ecdysone-insect-hormone-an-ind
Ecdysone Insect Hormone Switches on Disease
21 Jul 2013 | ECDYSONE
MORGELLONS:
INSECT HORMONE ECDYSONE TRIGGERS GENETIC CHANGES
TO BUILD NEW HAIR, SKIN, NAILS and CAUSE DISEASES
SkizitGesture-2013
Morgellons is a military grade entomological terror weapon being used to
torture and kill innocent civilians. The medical community has not been
informed about this disease and its complexity keeps it from being properly
identified, ... 阅读全帖 |
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i*****i
发帖数: 154 | 40 因实验需要求住下面这篇文献，谢谢。
Digestion. 2011;83(3):180-3. doi: 10.1159/000321809. Epub 2011 Jan 21.
FGF19 protects colonic epithelial cells against hydrogen peroxide.
Uchiyama K, Naito Y, Takagi T, Mizushima K, Hayashi N, Handa O, Ishikawa T,
Yagi N, Kokura S, Yoshikawa T.
Molecular Gastroenterology and Hepatology, Kyoto Prefectural University of
Medicine, Kamigyo-ku, Kyoto, Japan.
Pubmed链接
http://www.ncbi.nlm.nih.gov/pubmed/21266813
KARGER全文链接
http://www.karger.com/Article/FullText/321809
我的email：
microar... 阅读全帖 |
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t**l
发帖数: 109 | 41 有意思的是，不只是Chen在这个领域灌水，连结构大牛dinshaw patel灌了2篇cell从这
个领域
Structure-Function Analysis of STING Activation by c[G(2',5')pA(3',5')p] and
Targeting by Antiviral DMXAA.
Gao P, Ascano M, Zillinger T, Wang W, Dai P, Serganov AA, Gaffney BL, Shuman
S, Jones RA, Deng L, Hartmann G, Barchet W, Tuschl T, Patel DJ.
Cell. 2013 Aug 15;154(4):748-62. doi: 10.1016/j.cell.2013.07.023. Epub 2013
Aug 1.
PMID: 23910378 [PubMed - in process]
Cyclic [G(2',5')pA(3',5')p] is the metazoan second messenger produced by DNA
-... 阅读全帖 |
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l**********1
发帖数: 5204 | 42 非植物 或哺乳的数据库区别也 植物的也 一样在完善
关键是楼主有无NGS and HMM （Hidden Markov Models 的背景或能找到那种背景的
合作人
pls refer
PMID 23435661
by Van der Does D et al., (2013).
Salicylic acid suppresses jasmonic acid signaling downstream of SCFCOI1-JAZ
by targeting GCC promoter motifs via transcription factor ORA59.
Plant Cell. 25: 744-61.
Abstract:
ignored
In silico promoter analysis of the SA/JA crosstalk transcriptome revealed
that the 1-kb promoter regions of JA-responsive genes that are suppressed by
SA are significantl... 阅读全帖 |
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l**********1
发帖数: 5204 | 43 pls refer fresh new both papers:
a,
by Morozov VY and Ioshikhes IP. (2013).
Optimized Position Weight Matrices in Prediction of Novel Putative Binding
Sites for Transcription Factors in the Drosophila melanogaster Genome.
PLoS One. 8: e68712.
Abstract
Position weight matrices (PWMs) have become a tool of choice for the
identification of transcription factor binding sites in DNA sequences.
below ignored
In the present study, we extended this technique originally tested on
single examples of trans... 阅读全帖 |
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l**********1
发帖数: 5204 | 44 Pls refer one review
By Madrigal P and Krajewski P. (2012).
Current bioinformatic approaches to identify DNase I hypersensitive sites
and genomic footprints from DNase-seq data.
Front Genet. 3:230.
cited from its pp2:
>With sufficiently deepsequencing,the
>so-called“digital genomic footprinting”
>technique can reveal single protein-
>binding events(Hesselberth et al.,2009).
>Unlike ChIP-seq,which is specific for the
>protein under study,footprints identify
>narrow DNA regions that can be bound
>... 阅读全帖 |
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D*a
发帖数: 6830 | 45 PUBMED 也关门了
Due to the lapse in government funding, PubMed is being maintained with
minimal staffing. Information will be updated to the extent possible, and
the agency will attempt to respond to urgent operational inquiries. |
|
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l**********1
发帖数: 5204 | 47 cited,
>Both zinc finger nucleases (ZFNs) and TAL effector nucleases (TALENs)
>can be used to mutagenize genomes at specific loci, but
these systems require two different DNA binding proteins
flanking a sequence of interest, each with a C-terminal
FokI nuclease module. As a result these methods have not
been widely adopted by the plant research community.
from
>Belhaj K et al., (2013).
>Plant genome editing made easy: targeted mutagenesis in model and crop
plants using the CRISPR/Cas >system.
>P... 阅读全帖 |
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m**********n
发帖数: 299 | 48 http://www.ncbi.nlm.nih.gov/pubmed/?term=24014010
1: Garvey WT. New tools for weight-loss therapy enable a more robust medical
model for obesity treatment: rationale for a complications-centric approach.
Endocr Pract. 2013 Sep-Oct;19(5):864-74. doi: 10.4158/EP13263.RA. PubMed
PMID:
24014010.
非常感谢。 |
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m**********n
发帖数: 299 | 49 包子求助文献一篇
http://www.ncbi.nlm.nih.gov/pubmed/?term=24014010
1: Garvey WT. New tools for weight-loss therapy enable a more robust medical
model for obesity treatment: rationale for a complications-centric approach.
Endocr Pract. 2013 Sep-Oct;19(5):864-74. doi: 10.4158/EP13263.RA. PubMed
PMID:
24014010.
非常感谢。 |
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l**********1
发帖数: 5204 | 50 that slide ppt file didn’t include after 2010 fall to now papers,
pls check,
200 times of ordinary retroviral vector that High-titre retroviral vector
system
Wu C, Lu Y.
High-titre retroviral vector system for efficient gene delivery into human
and mouse cells of haematopoietic and lymphocytic lineages.
J Gen Virol. 2010 Aug;91: 1909-18.
Abstract
Genetically modified cells of haematopoietic and lymphocytic lineages could
provide potentially curative treatments for a wide range of inherited and
... 阅读全帖 |
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