N*o
发帖数: 97 | 1 try stb12 bacteria (grown at 30C); try promoter-less plasmid (pUC18, pUC19).
Or try keeping the insert in two plasmids (e.g. 2kb in one, 3kb in the
other) while cloning and finally ligate the two. good luck! |
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O******e
发帖数: 14 | 2 stb12? from which company? A simple search brought it to stratagene,but
nothing on their website. Thanks.
). |
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t******r
发帖数: 8600 | 3 stb12 is a piece of garbage
). |
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