t***u
发帖数: 230 | 1 It really depends on the GC content of your RNA. My suggestion is to shuffle
and generate a random pool (say, 100) of your RNA based on the GC content.
Then run them through mfold (the server can handle batch jobs, you just need
to wait for a while), and then you will have a normal distribution of the
folding free energy. Compare the folding free energy of your native sequence
to the distribution will show you whether it's better or worse than random. | r*****d
发帖数: 54 | 2 你可以用Vienna Package
计算secondary structure的
partition function,这样就可疑
得到这个结构在所有可能构象中
所占的比利.Vienna Package is free online!
另外,mfold server也可以给出
次忧的结构,我个人认为次忧的结构
也可以看出最忧结构是否稳定. |
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