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Biology版 - how to quantify <1ng/ul DNA/RNA w/o RT-PCR?
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进入Biology版参与讨论
1 (共1页)
w******e
发帖数: 1187
1
anything that can fill the gap b/w nanodrop and RT-PCR?
BTW, here I suppose the limiting factors are both concentration AND total
amount (say 30ul of <1ng/ul solution).
thx!
v*******a
发帖数: 759
2
run 10ul in agarose gel, stain with sybr green gold, compare to standard
w******e
发帖数: 1187
3
need everything for downstream experiment. so anything hugely destructive
is not an option (can only spare a few ul for quantification purpose-_-)

【在 v*******a 的大作中提到】
: run 10ul in agarose gel, stain with sybr green gold, compare to standard
s******r
发帖数: 2876
4
Picogreen may help.

anything that can fill the gap b/w nanodrop and RT-PCR?
BTW, here I suppose the limiting factors are both concentration AND total
amount (say 30ul of <1ng/ul solution).
thx!

【在 w******e 的大作中提到】
: anything that can fill the gap b/w nanodrop and RT-PCR?
: BTW, here I suppose the limiting factors are both concentration AND total
: amount (say 30ul of <1ng/ul solution).
: thx!

s*******g
发帖数: 3332
5
bioanalyzer pico

【在 w******e 的大作中提到】
: anything that can fill the gap b/w nanodrop and RT-PCR?
: BTW, here I suppose the limiting factors are both concentration AND total
: amount (say 30ul of <1ng/ul solution).
: thx!

b****o
发帖数: 193
m*****y
发帖数: 857
7
MassRuler by Fermentas

total

【在 w******e 的大作中提到】
: anything that can fill the gap b/w nanodrop and RT-PCR?
: BTW, here I suppose the limiting factors are both concentration AND total
: amount (say 30ul of <1ng/ul solution).
: thx!

1 (共1页)
进入Biology版参与讨论
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问一个比较白痴的问题,关于stop codon的引入包子请教:哪些技术能digitally quantify protein molecule number
请教有人做RACE吗?如何quantify in vitro transcription产量呢?
长片段PCRRe: 检测基因表达变化时,RT-PCR和Northern blot首选哪个?
请推荐PCR超长片段的polymerase目前SARS实验室诊断技术简介[zz]
RACE求助!!国内教授申请美国或欧洲博士后
any experience?能用real time RT-PCR来比较同一细胞的不同gene的含量吗?
相关话题的讨论汇总
话题: 1ng话题: rt话题: pcr话题: ul话题: dna