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Biology版 - oligo anneal and ligation
相关主题
新手求教:怎样把小于30bp的DNA fragment 连接到vector上用什么引物能够直接从RNA里把一个基因给扩出来?
土问:怎样提高two Oligo ligation的效率?问一下 aflII
Re: DNA microarry bioinformatics?Re: Help on Tm calculation
Re: 问个anneal linker的小问题求推荐一个合成大量修饰的DNA oligo的公司
primer的melting temperature请教:如何sequence biotinylated ss DNA oligo?
请教精确定量NH2 group的方法现在发文章还需要2套siRNA吗?
借人气问个小片段回收的问题Re: Ng-Ago 重复进展(集合贴)
Can someone recommend an oligo synthesis company?便宜好用的oligo合成公司
相关话题的讨论汇总
话题: oligo话题: anneal话题: ligation话题: ppl话题: 30bp
进入Biology版参与讨论
1 (共1页)
b******k
发帖数: 1874
1
for this topic, many ppl replied. I have compiled some of them here. (btw, s
ome of them are better than the one marked). while majority of ppl suggest t
o add Pi tto oligo 3' end by TNK, I don't know why. Anyone could help give r
elated mechanisms? Thanks a lot.
???: smartkevin (Biofuels), ??: Biology
? ?: Re: ????:?????30bp?DNA fragment ???vector?
???: BBS ????? (Tue May 18 04:01:41 2010, ??)
??oligo?????????????,???????????
??????protocol,??????:
1. oligos?TE or H2O??,??100 uM;
2. ???????,37
b******k
发帖数: 1874
2
i will post it again when back home. sorry, sounds my pc in schoool doesnt'
support chinese font.

s
t
r

【在 b******k 的大作中提到】
: for this topic, many ppl replied. I have compiled some of them here. (btw, s
: ome of them are better than the one marked). while majority of ppl suggest t
: o add Pi tto oligo 3' end by TNK, I don't know why. Anyone could help give r
: elated mechanisms? Thanks a lot.
: ???: smartkevin (Biofuels), ??: Biology
: ? ?: Re: ????:?????30bp?DNA fragment ???vector?
: ???: BBS ????? (Tue May 18 04:01:41 2010, ??)
: ??oligo?????????????,???????????
: ??????protocol,??????:
: 1. oligos?TE or H2O??,??100 uM;

1 (共1页)
进入Biology版参与讨论
相关主题
便宜好用的oligo合成公司primer的melting temperature
PNAS无可救药了,这样的烂文章也发……%#¥&#……请教精确定量NH2 group的方法
BioFuels领域牛牛最近有啥动静?借人气问个小片段回收的问题
Re: help on ligation problem!Can someone recommend an oligo synthesis company?
新手求教:怎样把小于30bp的DNA fragment 连接到vector上用什么引物能够直接从RNA里把一个基因给扩出来?
土问:怎样提高two Oligo ligation的效率?问一下 aflII
Re: DNA microarry bioinformatics?Re: Help on Tm calculation
Re: 问个anneal linker的小问题求推荐一个合成大量修饰的DNA oligo的公司
相关话题的讨论汇总
话题: oligo话题: anneal话题: ligation话题: ppl话题: 30bp