k*******i
发帖数: 49 | 1 先谢谢各位看官！
俺想知道有没有谁做IHC的时候没有perfusion，直接处理的fresh tissue？
谢谢啦 |
s******e
发帖数: 370 | 2 all depends on how big is your tissue
I rarely perfuse embryonic tissue |
h*******o
发帖数: 4884 | 3 I was under the impression that perfusion is mainly to get rid of the blood
or more specifically IgGs out of the tissue so there will be much less
background in IHC.
Correct me if I am wrong
For embryos, endogenous IgGs are relatively low compared to adult tissues.
Maybe that's why it works well without perfusion.
【在 s******e 的大作中提到】
: all depends on how big is your tissue
: I rarely perfuse embryonic tissue
|
j*********n
发帖数: 220 | 4 红细胞干扰荧光
可以直接frozen section，看你需要。
【在 k*******i 的大作中提到】
: 先谢谢各位看官！
: 俺想知道有没有谁做IHC的时候没有perfusion，直接处理的fresh tissue？
: 谢谢啦
|
k*******i
发帖数: 49 | 5 半个rat brain的大小
【在 s******e 的大作中提到】
: all depends on how big is your tissue
: I rarely perfuse embryonic tissue
|
k*******i
发帖数: 49 | 6 我是觉得如果直接frozen tissue，剩下的tissue还可以做别的protein assay。要是
perfusion的话，就不能了。 |
w*******s
发帖数: 10 | 7 perfusion with saline or PFA?
saline is to remove the blood from the tissue; and PFA is to fix the large
tissue that takes a long time to be fixed by immersion.
correct me if i'm wrong. |
n***w
发帖数: 2405 | 8 先saline把血洗了，然后perfuse with pfa。你会很明显地观察到一些特征表明各处基
本都fix了。。。这时候可以停了，取器官。。。 |
D*a
发帖数: 6830 | 9 小鼠大脑切片，反正我们做的明显perfusion的比直接扔pfa的好看。 |
