How to check if my plasmid may or may not be table in DH5a? - Biology版

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Biology版 - How to check if my plasmid may or may not be table in DH5a?

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话题: dh5a话题: may话题: plasmid话题: sequence话题: table

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m******5
发帖数: 1383

I am doing a large fragment(2.3) plus large vector(12k) ligation.
I have learned previously that some plasmids containing certain sequence may
not be stable in DH5a line, but I didn't find online about which kind of
sequence or plasmid would be unstable.
Does anyone here have experience on handling this problem?

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● sub-cloning真是揪心啊	● Cloning
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● 问个菜鸟问题	● 【虎年吉祥】请教牛人们克隆问题！
● about pBABE puro vector	● 请教一下现在是不是有些卖的plasmid只能用一次
● Maxiprep problem	● 怎么样寄plasmid去中国比较方便啊？？
● 关于质粒构建问题	● copy number of plasmids

相关话题的讨论汇总
话题: dh5a话题: may话题: plasmid话题: sequence话题: table

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