j*****a
发帖数: 92 | 1 ionic exchange column pools 浓缩后不经过dialysis直接上size exclusive column
是否会影响分离. |
I*****y
发帖数: 6402 | 2 I think it really depends on your buffer used for your IEX elution. I don't think a
sizing column is sensitive to salt.
If you still have concern with the salt from your IEX elution, just try
changing the buffer to low salt when you concentrate your proteins.
column
【在 j*****a 的大作中提到】
: ionic exchange column pools 浓缩后不经过dialysis直接上size exclusive column
: 是否会影响分离.
|
m**z
发帖数: 787 | 3 should be fine. I do this all the time. I think unless your protein's
oligomerization state is sensitive to salt concentration, otherwise salt
concentration should not affect the purification.
In fact I know some people actually using size exclusion column to do buffer
exchange instead of dialysis when the sample volume are small.
column
【在 j*****a 的大作中提到】
: ionic exchange column pools 浓缩后不经过dialysis直接上size exclusive column
: 是否会影响分离.
|
k******e
发帖数: 8870 | 4 no, it won't affect the seperation |
s********l
发帖数: 1195 | 5 对，盐浓度对尺寸排组影响不大，但是要保证出来的东西没有杂质会堵凝胶，上样前最
好过滤一下。
column
【在 j*****a 的大作中提到】
: ionic exchange column pools 浓缩后不经过dialysis直接上size exclusive column
: 是否会影响分离.
|
